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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (1): 64-68.doi: 10.3969/j.issn.0253-2417.2013.01.012

• 1研究报告 • 上一篇    下一篇

介孔氧化钛与海藻酸钠固定化木聚糖酶的对比研究

单宗星1, 韦策2, 李鑫1,3, 宋向阳1,3, 张杨1, 张伟炜1, 徐晨琛1   

  1. 1. 南京林业大学 化学工程学院, 江苏 南京 210037;2. 南京工业大学 生物与制药工程学院, 江苏 南京 211800;3. 江苏省生物质绿色燃料与化学品重点实验室, 江苏 南京 210037
  • 收稿日期:2012-09-24 修回日期:1900-01-01 出版日期:2013-02-28 发布日期:2013-02-28
  • 通讯作者: 李鑫,副教授,硕士生导师,从事生物质利用和生物转化研究;E-mail:xli@njfu.edu.cn。

Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers

SHAN Zong-xing1, WEI Ce2, LI Xin1,3, SONG Xiang-yang1,3, ZHANG Yang1, ZHANG Wei-wei1, XU Chen-chen1   

  1. 1. The College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;2. Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211800, China;3. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Received:2012-09-24 Revised:1900-01-01 Online:2013-02-28 Published:2013-02-28

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摘要： 为了提高木聚糖酶在应用过程中的稳定性,以介孔氧化钛和海藻酸钠为载体,开展木聚糖酶固定化研究。氧化钛固定化木聚糖酶,考察了吸附时间、给酶量、温度和pH值对固定化的影响;海藻酸钠固定化木聚糖酶,考察了NaCl浓度、海藻酸钠浓度和硬化时间对固定化的影响。在此基础上,对比两种固定化木聚糖酶的稳定性。研究结果表明,优化的介孔氧化钛固定化条件为:固定化时间0.5 h,给酶量20.36 IU/g,温度65 ℃,pH值6.0,固定化木聚糖酶回收率为93.37%;优化的海藻酸钠固定化条件为:硬化时间2 h,氯化钙质量浓度为20 g/L,海藻酸钠质量浓度为35 g/L,温度65 ℃,pH值6.0,固定化木聚糖酶回收率为28.77%。相对于海藻酸钠固定化木聚糖酶和游离木聚糖酶,介孔氧化钛固定化木聚糖酶热稳定性和酸碱稳定性均优于海藻酸钠固定化木聚糖酶及游离木聚糖酶。

关键词: 木聚糖酶, 固定化, 介孔氧化钛, 海藻酸钠, 稳定性

Abstract: In order to improve the stability of xylanase in biocatalysi, mesoporous titania (M-TiO2) and sodium alginate were used to immobilize xylanase. The properties of immobilized xylanase were also investigated in this study. The optimum immobilization by mesoporous titiania was obtained as 20.36 IU/g xylanase was immobilized at 65 ℃ for 0.5 h under pH 6.0. The maximal immobilized rate is 93.37%.In contrast, the xylanase was immobilized when 35 g/L sodium alginate was selected to handle the enzyme at 65 ℃ for 2 h under pH 6.0 environment in the presence of 20 g/L CaCl2. Maximal immobilized rate is 28.77%.In regard of the free and immobilized xylanase on sodium alginate, immobilized xylanase by mesoporous titania has better thermostability and pH stability.

Key words: xylanase, immobilization, mesoporous titania, sodium alginate, stability

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