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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (2): 91-94.doi: 10.3969/j.issn.0253-2417.2013.02.016

• 研究报告 • 上一篇    下一篇

利用玉米芯同步糖化发酵产2,3-丁二醇的研究

蒋兴, 夏黎明   

  1. 浙江大学 化学工程与生物工程系, 浙江 杭州 310027
  • 收稿日期:2012-04-06 修回日期:1900-01-01 出版日期:2013-04-30 发布日期:2013-04-30
  • 通讯作者: 夏黎明,教授,博士生导师,主要从事纤维素资源生物转化与利用方面的研究;E-mail:xialm@zju.edu.cn。

Production of 2,3-Butanediol from Corncob by Simultaneous Saccharification and Fermentation Process

JIANG Xing, XIA Li-ming   

  1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
  • Received:2012-04-06 Revised:1900-01-01 Online:2013-04-30 Published:2013-04-30

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摘要： 以玉米芯为原料,采用同步糖化发酵(SSF)工艺,将玉米芯酶水解及2,3-丁二醇发酵耦合在一起同步进行。通过对SSF主要工艺参数的研究,确立了适宜的工艺条件为:纤维素酶添加量25 FPIU/g(以底物计,下同),纤维二糖酶添加量15 IU/g,木聚糖酶添加量300 IU/g,底物质量浓度100～120 g/L,pH值6.0,36 ℃。底物质量浓度为120 g/L时,SSF周期36 h,2,3-丁二醇质量浓度可达46.02 g/L,产率为1.28 g/(L·h),转化率为0.424 g/g(以纤维素及半纤维素为参照)。

关键词: 3-丁二醇, 同步糖化发酵, 玉米芯, 纤维素, 半纤维素

Abstract: Production of 2,3-Butanediol from corncob was carried out by simultaneous saccharification and fermentation(SSF) process.The optimal SSF conditions were obtained as 100-120 g/L substrates were treated by 25 FPIU/g substrate cellulose, 15 IU/g substrate cellobiase and 300 IU/g substrate xylanase under pH 6.0 and 36 ℃ environment.The concentration,productivity and conversion rate of 2,3-butanediol were 46.02 g/L, 1.28 g/(L·h) and 0.424 g/g( based on cellulose and hemicellulose) respectively by SSF treatment for 36 h when the substrate concentration is 120 g/L.

Key words: 3-butanediol, simultaneous saccharification and fermentation, corncob, cellulose, hemicellulose

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