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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (2): 113-118.doi: 10.3969/j.issn.0253-2417.2013.02.020

• 研究报告 • 上一篇    下一篇

桉叶不饱和脂肪酸的提取研究及成分分析

杨巧丽1,2, 谢耀坚1, 谭晓风2, 张婧1, 黄赤夫3, 吴志华1   

  1. 1. 国家林业局桉树研究开发中心, 广东 湛江 524022;2. 中南林业科技大学经济林育种与栽培国家林业局重点实验室, 湖南 长沙 410004;3. 肯塔基大学医学中心口腔学院,肯塔基州 莱克星顿 40503
  • 收稿日期:2012-03-28 修回日期:1900-01-01 出版日期:2013-04-30 发布日期:2013-04-30
  • 通讯作者: 吴志华(1974-),男,高级工程师,主要从事植物生理生化研究;E-mail:wzhua2889@163.com。

Extraction of Unsaturated Fatty Acids from Eucalyptus Leaves and its Components

YANG Qiao-li1,2, XIE Yao-jian1, TAN Xiao-feng2, ZHANG Jing1, HUANG Chi-fu3, WU Zhi-hua1   

  1. 1. Eucalypt Research Centre, Zhanjiang 524022, China;2. The Key Lab of Non-wood Forest Products of State Forestry Administration, Central University of Forestry and Technology, Changsha 410004, China;3. College of Dentistry, Medicine Center, University of Kentucky, Lexington 40503, USA
  • Received:2012-03-28 Revised:1900-01-01 Online:2013-04-30 Published:2013-04-30

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摘要： 用不同的溶剂、物料粒度、料液比、浸泡时间及萃取时间对桉叶不饱和脂肪酸进行提取的正交试验,筛选出最优的提取方案并对提取物甲酯化处理后通过GC-MS分析其成分。在进行了L16(45)正交试验之后,研究得到当溶剂为丙酮、物料粒度为0.075～0.15 mm、料液比为1:15(g:mL)、浸泡时间为8 h及超声波处理1.5 h时提取得率为14.9 %,GC-MS分析,鉴定出6个化合物,其中有5个脂肪酸类化合物,如不饱和脂肪酸亚油酸和亚麻酸,10 g桉叶中含有36.01 mg不饱和脂肪酸。桉叶中含有重要的不饱和脂肪酸,具有开发利用价值。

关键词: 桉叶, 不饱和脂肪酸, 正交试验, 提取, 成分分析

Abstract: The optimal extraction process of unsaturated fatty acids from Eucalyptus leaves was obtained by orthogonal test based on factors such as different solvents,material particle size,solid-liquid ratio,immersion time and extraction time. The components of extracts were analysed by GC-MS after methylation. The results from 16 tests of 5 factors and 4 levels showed that the extraction yield was 14.9 % on the optimal extraction conditions, i.e., acetone as solvent, material particle size 0.075-0.150 mm, solid-liquid ratio 1:15(g:mL), immersion time 8 h and extraction time 1.5 h. Six compounds including five fatty acids, for example unsaturated fatty acids, linoleic acid and linolenic acid, were indentified. There was 36.01 mg unsaturated fatty acids in 10 g dried Eucalyptus leaves. Eucalyptus leaves have potential values to develop because of unsaturated fatty acids.

Key words: Eucalyptus leaves, unsaturated fatty acid, orthogonal test, extraction, composition analysis

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