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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (4): 73-78.doi: 10.3969/j.issn.0253-2417.2013.04.014

• 1研究报告 • 上一篇    下一篇

超声波提取核桃内种皮多酚的响应面优化及其抗氧化研究

周晔1,2, 王伟1, 陶冉2, 陈虹霞2, 王成章2, 裴东1   

  1. 1. 中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 北京 100091;2. 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局林产化学工程重点开放性实验室; 江苏省生物质能源与材料重点实验室, 江苏 南京 210042
  • 收稿日期:2012-06-14 修回日期:1900-01-01 出版日期:2013-08-30 发布日期:2013-08-30
  • 通讯作者: 裴 东,研究员,博士生导师,从事植物生物技术研究;E-mail:peigu@caf.ac.cn; 王成章,研究员,博士生导师,主要从事天然产物化学和加工利用研究;E-mail:wangczlhs@sina.com。

Optimization of Extraction of Phenolics from Walnut Pellicle Using Response Surface Methodology and Their Antioxidant Capacities

ZHOU Ye1,2, WANG Wei1, TAO Ran2, CHEN Hong-xia2, WANG Cheng-zhang2, PEI Dong1   

  1. 1. Rsearch Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;2. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
  • Received:2012-06-14 Revised:1900-01-01 Online:2013-08-30 Published:2013-08-30

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摘要： 以核桃内种皮酚类物质提取得率为响应值,设计提取温度、提取时间以及液固比3因素3水平的Box-Behnken试验,采用响应面法确定超声波提取核桃内种皮酚类物质的最佳工艺,同时通过自由基清除能力和还原力试验,评价内种皮酚类物质的抗氧化性能。结果表明: 0.1g核桃内种皮粉末在固定超声波功率为100W下的最佳提取工艺为提取温度53℃,提取时间55min,液固比58:1(mL:g),总多酚得率为30.79%。相同浓度下的核桃内种皮酚类物质抗氧化性明显高于天然抗氧化剂VC,其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的半数抑制浓度(IC50)值为3.38mg/L,小于Vc的4.72mg/L;清除2,2'-联氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)自由基的IC50值为36.44mg/L,小于Vc的82.23mg/L。

关键词: 核桃内种皮, 酚类物质, 响应面法, 抗氧化

Abstract: Using temperature, time and liquid/solid ratio as independent variables, a Box-Behnken design was applied to optimise the extraction of phenolics from walnut pellicle by using response surface methodology. The results indicated that when the ultrasonic power was set at 100 W, the optimum ultrasonic extraction conditions for walnut pellicle phenolics were as follows: extraction temperature 53℃,extraction time 55 min,liquid/solid ratio 58:1(mL:g). Under this optimum condition, the extraction yield was 30.79%. It was found that the phenolics extracted from walnut pellicle behaved stronger antioxidant activities than ascorbic acid when they were under the same concentration. The IC50 values of scavenging activity of pellicle phenolics on DPPH free radicals and ABTS were 3.38 mg/L and 36.44 mg/L,respectively,while those of ascorbic acid were 4.72 mg/L and 82.23 mg/L, respectively.

Key words: walnut pellicle, phenolics, response surface methodology, antioxidant

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