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林产化学与工业 ›› 2013, Vol. 33 ›› Issue (6): 14-18.doi: 10.3969/j.issn.0253-2417.2013.06.003

• 1研究报告 • 上一篇    下一篇

Sigmoidal拟合曲线法研究银杏叶类脂轻馏分的抗氧化性作用

陶冉1, 王成章1,2, 孔振武1,2   

  1. 1. 中国林业科学研究院 林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局 林产 化学工程重点开放性实验室; 江苏省 生物质能源与材料重点实验室, 江苏 南京 210042;2. 中国林业科学研究院 林业新技术研究所, 北京 100091
  • 收稿日期:2012-12-20 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
  • 通讯作者: 王成章(1966-),男,湖北汉川人,研究员,博士,博士生导师,主要从事天然产物研究与利用;E-mail:wangczlhs@sina.com。

Antioxidant Effect of Light Distillates Separated by Molecular Distillation from Lipids of Ginkgo Biloba L. Leaves Using Sigmoidal Curve Fitting Method

TAO Ran1, WANG Cheng-zhang1,2, KONG Zhen-wu1,2   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042,China;2. Institute of New Technology of Forestry, CAF, Beijing 100091, China
  • Received:2012-12-20 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

摘要: 通过石油醚提取、皂化、分子蒸馏以及硅胶柱色谱分离等方法得到了7组银杏叶类脂不同极性轻馏分,用4种抗氧化测定方法(DPPH、ABTS、超氧阴离子和羟自由基法),结合Sigmoidal拟合曲线法求银杏叶类脂不同极性轻馏分的半数清除浓度(IC50),来评价银杏叶类脂轻馏分的抗氧化作用。结果显示,银杏叶类脂轻馏分具有一定抗氧化作用,其中馏分2(95%石油醚/5%乙酸乙酯部分)在各馏分组中抗氧化性最强,IC50分别为(51.0±0.061),(51.3±0.042),(56.9±0.045)和(124.4±0.037)mg/L;所有馏分样品中偏离最大的清除率实验值与清除率为50%的相对偏差为1.4%,表明Sigmoidal拟合曲线法求银杏叶类脂轻馏分不同极性馏分的IC50是合理的。

关键词: 银杏, Sigmoidal拟合曲线法, 类脂, 抗氧化性

Abstract: The fractions separated from light distillates of ginkgo lipids were prepared by extraction with petroleum ether, saponification, molecular distillation and silica gelcolumn chromatographic isolation. Four methods (DPPH, ABTS, superoxide anion and hydroxy radical) with Sigmoidal curve fitting method arriving at respective IC50 values were used to determine the antioxidant effect of the seven fractions. The results showed that light distillates of ginkgo lipids had antioxidant effect and the fraction 2 (95% petroleum ether/5% acetic ether) had the highest antioxidant effect in all tested fractions. IC50 values were (51.0±0.061), (51.3±0.042), (56.9±0.045) and (124.4±0.037)mg/L. The relative deviation between experimental scavenging rate and scavenging rate (50%) of the fraction having maximum deviation in all fractions was 1.4%. Consequently, it is reasonable that the IC50 values of different polar fractions of light distillates separated from ginkgo lipids are generated with Sigmoidal curve fitting method.

Key words: Ginkgo biloba, Sigmoidal curve fitting method, lipids, antioxidant

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