欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (6): 101-105.doi: 10.3969/j.issn.0253-2417.2013.06.019

• 1研究报告 • 上一篇    下一篇

正交试验法优选雪松落叶中总黄酮的研究

冯世敬1, 李红萍2, 高静1, 徐丽1, 刘国际1   

  1. 1. 郑州大学 化工与能源学院, 河南 郑州 450001;2. 郑州大学 公共卫生学院, 河南 郑州 450001
  • 收稿日期:2012-11-02 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
  • 通讯作者: 刘国际，博士生导师，研究领域为化学反应工程。

Optimization of the Extraction Technology of Flavonoids from Fallen Pine Needles by Orthogonal Experiment

FENG Shi-jing1, LI Hong-ping2, GAO Jing1, XU Li1, LIU Guo-ji1   

  1. 1. School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China;2. College of Public Health, Zhengzhou University, Zhengzhou 450001, China
  • Received:2012-11-02 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

PDF (PC)

661

摘要： 采用正交试验设计，对亚临界流体提取雪松松针落叶中总黄酮萃取工艺的影响因素进行了考察。实验以紫外分光光度法测定松针中总黄酮含量，以芦丁为参照品，在波长 360 nm 处测定总黄酮含量。结果表明，影响总黄酮萃取的主次因素为：萃取温度>萃取时间>液料比>萃取压力。总黄酮萃取的较优水平为：萃取温度 60 ℃、萃取压力 4 MPa、萃取时间 60 min、液料比为3：1（mL：g），在该萃取条件下，总黄酮萃取量可达 6.677 mg/g。

关键词: 松针落叶, 亚临界流体, 总黄酮

Abstract: The extraction conditions for subcritical fluid extraction of flavonoids from the cedar fallen pine needles were studied by orthogonal experimental design. In this paper, the total flavonoids content in pine needles was determined by UV Spectrophotometry. The contrast sample was rutin and the analyzed wavelength was 360 nm. The results showed that, the sequence of the impact of various factors on the extraction was as follows: extraction temperature >extraction time >liquid-material ratio >extraction pressure.The excellent levels of extraction of total flavonoids were as follows: extraction temperature 60 ℃, extraction pressure 4 MPa, extraction time 60 min, liquid-material ratio for the 3:1. Under these extraction conditions, the extraction amount of flavonoids rose up to 6.677 mg/g.

Key words: fallen pine needles, subcritical fluid, total flavonoids

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3