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林产化学与工业 ›› 2014, Vol. 34 ›› Issue (1): 107-112.doi: 10.3969/j.issn.0253-2417.2014.01.018

• 研究报告 • 上一篇    下一篇

超声波辅助减压提取枣皮多酚的研究

游凤1, 黄立新1,2, 张彩虹1, 谢普军1, 张耀雷1   

  1. 1. 中国林业科学研究院 林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局 林产 化学工程重点开放性实验室; 江苏省 生物质能源与材料重点实验室, 江苏 南京 210042;
    2. 中国林业科学研究院 林业新技术研究所, 北京 100091
  • 收稿日期:2013-01-18 出版日期:2014-02-25 发布日期:2014-03-05
  • 通讯作者: 黄立新,研究员,博士,研究领域:林特产品深加工及干燥技术;E-mail:l_x_huang@163.com。 E-mail:l_x_huang@163.com
  • 作者简介:游凤(1988-),女,湖北黄石人,硕士生,主要从事天然产物深加工研究工作
  • 基金资助:
    国家林业局948技术引进项目(2012-4-12);国家863计划资助(2011AA100802-9)

Ultrasound-assisted Vacuum Extraction of Polyphenols from Jujube Peel

YOU Feng1, HUANG Lin-xin1,2, ZHANG Cai-hong1, XIE Pu-jun1, ZHANG Yao-lei1   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
    2. Institute of New Technology of Forestry, CAF, Beijing 100091, China
  • Received:2013-01-18 Online:2014-02-25 Published:2014-03-05

摘要: 以陕西长枣皮为原料,采用超声波减压法提取其中多酚,通过单因素试验及正交试验L9(34)考察了各因素间的交互作用,优化了超声波辅助减压提取枣皮多酚的条件。在此基础上,对比了常规浸提、超声浸提和超声波辅助减压提取三者对提取得率和提取时间的影响,并通过清除自由基能力(DPPH·)衡量了各种提取方法的优劣。结果表明:超声波辅助减压提取枣皮多酚的最佳工艺参数为乙醇体积分数 50 %,提取温度 55 ℃,液料比30:1(mL:g),超声波功率 240 W,提取时间 10 min,枣皮多酚得率可达 5.05 %,此条件下提取2次,多酚得率为6.33%,提取率即达 97.37 %;对比热浸提和超声波浸提,超声波辅助减压提取在明显缩短提取时间的同时又能显著提高枣皮多酚得率;清除自由基能力(DPPH·)测试表明超声波辅助减压提取所得的多酚粗提物活性最高,清除率88.4%,该方法能够更好的保护枣皮多酚的生物活性。

关键词: 枣皮, 多酚, 超声波辅助减压提取, 清除自由基

Abstract: In order to obtain maximum yield of polyphenols from jujube peel, ultrasound-assisted vacuum extraction (UAVE) was chosen to extract the peel of Ziziphus jujube var. Changzao. The effects of various conditions were investigated by single factor experiments and orthogonal design L9(34). Based on the optimization condition, three extraction methods, i.e., UAVE, ultrasound assist extraction(UAE) and conventional extraction(CE), were compared with both extraction time and polyphenols yield. To further evaluate UAVE, the radical scavenging activities (DPPH·) was measured, too. Results showed that: the optimal conditions were 50 % aqueous ethanol as extraction solvent, temperature 55 ℃, solid/liquid ratio 1:30(g:mL), ultrasonic power 240 W, extraction time 10 min. Under this condition, the polyphenols yield could be 5.05 %, and the extraction rate could reach 97.37 % with duplicate extraction. UAVE has advantages such as high yield and time saving by comparison with CE and UAE. The polyphenols extracted by UAVE showed that higher antioxidant activities could be obtained than those by CE and UAE. It indicated that UAVE could protect the bioactive capacity of polyphenols from jujube peel.

Key words: jujube peel, polyphenols, ultrasound-assisted vacuum extraction (UAVE), radical scavenging

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