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林产化学与工业 ›› 2014, Vol. 34 ›› Issue (4): 90-96.doi: 10.3969/j.issn.0253-2417.2014.04.015

• 研究报告 • 上一篇    下一篇

漆酶-超声波协同处理碱木质素的表面化学结构特征

连海兰1, 程明娟1, 洪枢1, 詹先旭2   

  1. 1. 南京林业大学 木材工业学院, 江苏 南京 210037;
    2. 德华兔宝宝装饰新材股份有限公司, 浙江 湖州 313200
  • 收稿日期:2014-01-10 出版日期:2014-08-25 发布日期:2015-08-18
  • 作者简介:连海兰(1971-),女,山东威海人,教授,博士,硕士生导师,研究领域为生物质复合材料、胶黏剂与涂料;E-mail:lianhailan@njfu.edu.cn。
  • 基金资助:
    国家自然科学基金资助项目(313707567);江苏省自然科学基金资助项目(BK2013427);江苏高校优势学科建设工程资助项目(无编号)

Surface Chemical Structure of Alkali Lignin Modified by the Synergistic Process of Laccase and Ultrasound

LIAN Hai-lan1, CHENG Ming-juan1, HONG Shu1, ZHAN Xian-xu2   

  1. 1. College of Wood Science and Technology, Nanjing Forestry University, Nanjing 210037, China;
    2. Dehua TB Decoration Material Co., Ltd., Huzhou 313200, China
  • Received:2014-01-10 Online:2014-08-25 Published:2015-08-18

摘要: 分别采用超声波、漆酶及超声波协同漆酶的方法处理麦草碱木质素,通过化学分析法结合红外光谱、X射线光电子能谱和电子自旋共振等仪器分析手段,对比各处理过程木质素酚羟基含量、表面官能团、表面元素及表面自由基的变化,探讨3种处理方式对碱木质素结构的影响机理。结果表明,超声波处理后的木质素,总酚羟基含量比未处理的有所增加,漆酶处理后酚羟基含量减少。与未经处理的纯碱木质素相比,木质素经超声波处理后自由基浓度减少了38%,而漆酶处理后自由基浓度显著增加,其中单一漆酶处理增加了112%,超声波后再进行酶处理增加了193%。超声波空化作用可使木质素发生脱甲氧基反应及连接键断裂;漆酶处理过程中有解聚反应,同时酚羟基氧化变成苯氧自由基,进而发生自由基反应;超声波后生成的木质素小分子更易与漆酶反应,C-O单键被氧化。

关键词: 超声波, 漆酶, 碱木质素, 结构特征

Abstract: Wheat alkali lignin was treated by ultrasound, laccase, and the synergistic process of ultrasound and lassase, respectively. The chemical structure of wheat alkali lignin after treatment was then characterized by the content of phenolic hydroxyl, infrared spectroscopy, X-ray photoelectron spectroscopy (XPS) and electron spin resonance (ESR). The result showed that comparing with the untreated lignin, the total phenolic hydroxyl content increased after ultrasonic treatment. However, it decreased after laccase treatment. The number of spin decreased by 38% after ultrasonic treatment, but it increased significantly after laccase treatment. Particularly, the number of spin increased by 112% after the single treatment by laccase. Then ultrasonic wave and laccase were applied to treat the sample in sequence. This resulted in the increase of the number of spin by 193%. In these cases, ultrasonic cavitation can induce the demethylation and cleavage of some linkages in lignin. Laccase was able to promote depolymerization. At the same time, phenolic hydroxyl in lignin was oxidized and generated phenoxy radicals for free radical polymerization during the process of laccase-catalyzed oxidation. Small molecule lignin obtained after ultrasonic treatment was prone to react with laccase and C-O was oxidated in this case.

Key words: ultrasonic, laccase, alkali lignin, structural characterization

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