欢迎访问《林产化学与工业》,

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (4): 107-112.doi: 10.3969/j.issn.0253-2417.2014.04.018

• 研究报告 • 上一篇    下一篇

亚临界水提取协同大孔树脂纯化杨树芽总黄酮

薄采颖1, 郑光耀1, 高丽萍1, 张建华2, 戴海静2   

  1. 1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局 林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏 南京 210042;
    2. 江苏华安科研仪器有限公司, 江苏 海安 226600
  • 收稿日期:2013-06-03 出版日期:2014-08-25 发布日期:2015-08-18
  • 作者简介:薄采颖(1984-),女,江苏东海人,助理研究员,硕士,从事植物资源提取分离与开发利用研究;E-mail:newstar2002@163.com
  • 基金资助:
    江苏省产学研前瞻性联合研究项目(BY2011116)

Extraction and Purification of Flavonoids from Poplar Buds with Subcritical Water Extraction Coordinated with Macroporous Resin Adsorption

BO Cai-ying1, ZHENG Guang-yao1, GAO Li-ping1, ZHANG Jian-hua2, DAI Hai-jing2   

  1. 1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jaingsu Province, Nanjing 210042, China;
    2. Jiangsu Huaan Scientific Research Devices Co., Ltd., Hai'an 226600, China
  • Received:2013-06-03 Online:2014-08-25 Published:2015-08-18
  • Contact: 郑光耀,副研究员,从事植物资源提取分离与开发利用研究;E-mail:zhguya@sina.com。 E-mail:zhguya@sina.com

摘要: 采用亚临界水提取-大孔树脂纯化联用技术对杨树芽中总黄酮进行提取、纯化,以提取温度、液料比和提取时间为考察因素,以总黄酮的得率为考察指标,采用正交试验优化亚临界水对杨树芽总黄酮的提取工艺;从4种大孔吸附树脂中筛选出对总黄酮有最佳分离纯化效果的一种树脂,研究其对总黄酮静态和动态吸咐以及解吸效果。结果表明,5 MPa下亚临界水提取杨树芽总黄酮的最佳工艺条件为:提取温度 180℃、提取时间 10 min、液料比30:1(mL:g),总黄酮的粗品提取得率为 11.83%,纯度为 13.16%。通过静态吸附试验筛选出最有效的HP-20树脂分离纯化杨树芽总黄酮,通过动态吸附试验确定应用HP-20树脂吸附分离杨树芽总黄酮的最佳工艺条件为:上样质量浓度为 4 g/L,流速为 60 mL/h,pH值为2~3,此时吸附量为42.69 g/L洗脱剂乙醇的体积分数为 90%,解吸率为93.91%,经纯化后总黄酮的纯度为 49.28%。

关键词: 杨树芽, 亚临界水, 总黄酮, 大孔树脂

Abstract: Subcritical water extraction coupled with macroporous adsorption resin technology was applied to extract and purify flavonoids from poplar buds. Based on the yield of flavonoids, the factors of extraction technology including extraction temperature, liquid-feed ratio was optimized through orthogonal tests. By comparison of four macroporous resins in the adsorption of flavonoids, the optimal macroporous of resin was selected, then adsorption and desorption effects were investigated. The results showed that the optimum process of subcritical water extraction were extraction temperature 180℃, liquid-feed ratio 30:1, extraction time 10 min. The yield of crude flavonoids was 11.83% and the purity of the product is 13.16%. The HP-20 macroporous adsorption resin was selected to separate and purify flavonoids from poplar buds. The optimal technological conditions were solution concentration 4 g/L, flow rate 60 mL/h, solution pH 2-3, and eluting solvent 90% ethanol. The purity of flavonoids can reach 49.28%.

Key words: poplar buds, subcritical water, flavonoids, macroporous adsorption resin

中图分类号: