减压内部沸腾法提取壶瓶枣多糖及其脱色工艺的研究

doi:10.3969/j.issn.0253-2417.2015.02.015

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (2): 97-102.doi: 10.3969/j.issn.0253-2417.2015.02.015

减压内部沸腾法提取壶瓶枣多糖及其脱色工艺的研究

张耀雷¹, 黄立新¹, 张彩虹^1,2, 谢普军¹, 游凤¹, 许乐¹

1. 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局林产化学工程重点开放性实验室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 中国林业科学研究院林业新技术研究所, 北京 100091

收稿日期:2014-01-09 出版日期:2015-04-25 发布日期:2015-04-24
通讯作者: 黄立新,研究员,博士生导师,主要从事天然产物提取分离纯化及新型干燥技术研究;E-mail:l_x_huang@163.com。 E-mail:l_x_huang@163.com
作者简介:张耀雷(1989-),男,江苏连云港人,硕士生,主要从事农林产品深加工研究工作
基金资助:
国家林业局948技术引进项目(2012-4-12);中国林科院林业新技术所基本科研业务费专项资金(CAFINT2013C04)

Extraction of Polysaccharides from Ziziphus Jujube Mill. cv. Hupingzao by Reduced Pressure Boiling Method and Its Decoloration Technology

ZHANG Yao-lei¹, HUANG Li-xin¹, ZHANG Cai-hong^1,2, XIE Pu-jun¹, YOU Feng¹, XU Le¹

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China

Received:2014-01-09 Online:2015-04-25 Published:2015-04-24

摘要/Abstract

摘要： 对减压内部沸腾法提取壶瓶枣多糖及其脱色工艺进行了研究。以蛋白质和多糖得率为指标,通过正交试验优化得到的最佳工艺条件为:体系内温度60 ℃,液料比为20:1(mL:g),时间为30 min,此时真空度为80 kPa,外界温度为70 ℃,蛋白质和多糖得率分别为0.13%和2.60%。与传统热浸提相比,其得率分别提高了18.18%和23.22%。对10种脱色材料的筛选结果表明D900型大孔吸附树脂是理想脱色材料,通过正交试验优化得到的D900型大孔吸附树脂脱色优化工艺参数为D900添加量3.5%,时间20 h,pH值11.7,温度40 ℃,此时脱色率、脱蛋白率和多糖保留率分别为81.94%、38.19%和87.42%。

关键词: 壶瓶枣, 减压内部沸腾法, 蛋白质, 多糖, 脱色

Abstract: Extraction of polysaccharides from Zizyphus jujube Mill. cv. Hupingzao by reduced pressure boiling method and its decoloration technology were studied in this paper.According to protein and polysaccharide yield, the optimum technological conditions were studied by orthogonal test. The results showed that the optimum technological conditions were system temperature 60 ℃, water/solid ratio 20:1(mL:g), time 30 min, vacuum degree 80kPa, and external temperature 70 ℃. Under these conditions, the protein and polysaccharides yields were 0.13% and 2.60%, respectively. Compared with traditional water extraction, the protein and polysaccharides yields were improved by 18.18% and 23.22%, respectively. According to the decolorization ratio, deproteinization ratio and retention rate of polysaccharides, macroporous adsorption resin D900 was the suitable one among 10 kinds of decolorization materials. Orthogonal test was employed to optimize the decolorization conditions. The results indicated that optimum decolorization conditions were additive amount of D900 3.5%, time 20 h, pH value 11.7 and temperature 40 ℃, respectively. Under these conditions, the decolorization ratio, deproteinization ratio, and retention rate of polysaccharides were 81.94%, 38.19%, and 87.42%, respectively.

Key words: Zizyphus jujube Mill. cv. Hupingzao, reduced pressure boiling method, protein, polysaccharides, decolorization

中图分类号:

TQ35

张耀雷, 黄立新, 张彩虹, 谢普军, 游凤, 许乐. 减压内部沸腾法提取壶瓶枣多糖及其脱色工艺的研究[J]. 林产化学与工业, 2015, 35(2): 97-102.

ZHANG Yao-lei, HUANG Li-xin, ZHANG Cai-hong, XIE Pu-jun, YOU Feng, XU Le. Extraction of Polysaccharides from Ziziphus Jujube Mill. cv. Hupingzao by Reduced Pressure Boiling Method and Its Decoloration Technology[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 97-102.

参考文献

[1] 李杰, 梁长利, 米芳.水溶性红枣多糖提取工艺条件的优化[J], 食品研究与开发, 2009, 30(9): 58-60. LI Jie, LIANG Chang-li, MI Fang.Study on the extraction conditions of water-soluble polysaccharides from jujube[J].Food Research and Development, 2009, 30(9):58- 60.
[2] ZHAO Zhi-hui, DAI Hui, WU Xiang-mei, et al.Characterization of a pectic polysaccharide from fruit of Ziziphus jujuba[J].Chemistry of Natural Compounds, 2007, 43(4):374-376.
[3] WANG Bing.Chemical characterization and ameliorating effect of polysaccharide from Chinese jujube on intestine oxidative injury by ischemia and reperfusion[J].International Journal of Biological Macromolecules, 2011, 48:386-391.
[4] LI Jin-wei, SHAN Liang, LIU Yuan-fa, et al.Screening of a functional polysaccharide from Zizyphus Jujuba cv. Jinsixiaozao and its property[J].International Journal of Biological Macromolecules, 2011, 49(3):255-259.
[5] 游凤, 黄立新, 张彩虹, 等.减压法提取长枣枣皮中的天然色素[J].食品与发酵工业, 2012, 38(12):169-174. YOU Feng, HUANG Li-xin, ZHANG Cai-hong, et al.Optimization of red pigments extraction from jujube peel (Zizihus jujube Mill.) under reduced pressure[J].Food and Fermentation Industries, 2012, 38(12):169-174.
[6] 谢普军, 黄立新, 张彩虹, 等.低温减压沸腾提取橄榄苦苷的工艺研究[J].中国中药杂志, 2012, 37(13):1946-1951. XIE Pu-jun, HUANG Li-xin, ZHANG Cai-hong, et al.Boiling extraction of oleuropein at low temperature and reduced pressure[J].China Journal of Chinese Materia Medica, 2012, 37(13):1946-1951.
[7] 唐岚, 计燕萍, 钱建华, 等.三生合欢饮减压提取工艺研究[J].中草药, 2013, 44(14):1918-1924. TANG Lan, JI Yan-ping, QIAN Jian-hua, et al.Study on decompression extraction of Sansheng Hehuan decoction[J].Chinese Traditional and Herbal Drugs, 2013, 44(14):1918-1924.
[8] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry, 1976, 72:248-254.
[9] 庄永亮, 孙丽平, 尚小丽.红托竹荪菌盖多糖的提取及抗氧化能力的研究[J].林产化学与工业, 2011, 31(3):45-49. ZHUANG Yong-liang, SUN Li-ping, SHANG Xiao-li.Extraction and antioxidant activities of polvsaccharides from pileus of Dictyophora rubrovalvata[J].Chemistry and Industry of Forest Products, 2011, 31(3):45-49.
[10] 王润地, 王中来, 叶建江.冷电弧在大枣多糖脱色中的应用[J].食品工业科技, 2010, 31(3):271-274. WANG Run-di, WANG Zhong-lai, YE Jian-jiang.Discolorization of polysaccharides from jujube by non-thermal plasma[J].Science and Technology of Food Industry, 2010, 31(3):271-274.
[11] 荣娟娟, 高家荣, 方朝晖, 等.多指标综合评分法优选芪归糖痛宁颗粒提取工艺[J].安徽医药, 2011, 15(6):674-676. RONG Juan-juan, GAO Jia-rong, FANG Chao-hui, et al.Optimization of the extraction technology for qiguitangtongning granules with the multiple guidelines grading method[J].Anhui Medical and Pharmaceutical Journal, 2011, 15(6):674-676.
[12] 刘丹, 李俊松, 李伟东, 等.综合评分法优选煅赭石炮制工艺[J].中成药, 2009, 31(3):424-426. LIU Dan, LI Jun-song, LI Wei-dong, et al.Optimization of the processing technology for calcined ocher with the multiple guidelines grading method[J].Chinese Traditional Patent Medicine, 2009, 31(3):424-426.

[1]	许嘉琍, 张海波, 姚姝凤, 高宏, 商士斌. 林业生物质基果蔬涂膜保鲜剂的研究进展[J]. 林产化学与工业, 2019, 39(3): 10-16.
[2]	张笮晦, 钱信怡, 黄广智, 毕良武. 肉桂和同源根皮不同极性部位的成分分析及其抑菌效果[J]. 林产化学与工业, 2019, 39(3): 115-122.
[3]	冯书晓, 张洪涛, 王水永, 胡永鑫, 朱培文, 尹卫平. 无花果果实水溶性成分的分离与鉴定[J]. 林产化学与工业, 2019, 39(2): 33-38.
[4]	张黎明, 李荣雪, 何希宏, 郝利民, 何志娜, 戴玉杰. 麻栎多糖的提取、纯化及其性质分析[J]. 林产化学与工业, 2019, 39(2): 59-66.
[5]	全熙宇, 彭湃, 文沛瑶, 魏锦锦, 张军华. 杜仲叶多糖、杜仲精粉、杜仲胶的提取分离及其性能分析[J]. 林产化学与工业, 2019, 39(2): 122-128.
[6]	刘丹丹, 韩唯凡, 蒋建新, 朱莉伟. 无患子果皮多糖微波提取工艺及抗氧化活性研究[J]. 林产化学与工业, 2018, 38(4): 109-116.
[7]	李雪, 王宏伟, 郑东然, 张鸿宇, 王英逵. 杜香叶多糖的提取及抗氧化和抗肿瘤活性研究[J]. 林产化学与工业, 2017, 37(5): 133-138.
[8]	刘彦涛, 张威伟, 章伟岸, 王堃, 孙达峰, 蒋建新. 微水固相法皂荚多糖胶羧甲基化改性[J]. 林产化学与工业, 2017, 37(4): 45-50.
[9]	蒋建新, 刘彦涛, 周自圆, 段久芳, 孙达峰. 高分子多糖水凝胶功能材料研究与应用进展[J]. 林产化学与工业, 2017, 37(2): 1-10.
[10]	王大伟, 赵宁, 季梅, 冯小飞, 户连荣. 云南苦樱桃树胶单糖成分分析及其接枝共聚物的合成与性质[J]. 林产化学与工业, 2017, 37(2): 65-71.
[11]	许小向, 尹小莉, 洪艳平, 邹凯, 上官新晨, 梁强. 火炬松松针多糖提取工艺及其抗氧化性研究[J]. 林产化学与工业, 2016, 36(3): 114-120.
[12]	张耀雷, 黄立新, 张彩虹, 谢普军, 张琼, 丁莎莎. 超声波喷雾干燥壶瓶枣多糖及其对产品品质的影响[J]. 林产化学与工业, 2016, 36(2): 64-70.
[13]	谢普军, 黄立新, 张彩虹, 游凤, 张耀雷. 不同菌种对油橄榄叶发酵饲料的影响[J]. 林产化学与工业, 2015, 35(4): 59-64.
[14]	章伟岸, 段久芳, 孙达峰, 张卫明, 蒋建新. 微水固相法氧化改性皂荚多糖胶[J]. 林产化学与工业, 2014, 34(6): 44-48.
[15]	冯君锋, 蒋剑春, 徐俊明, 贺小亮, 杨中志, 许乐. 生物质液化油分级产物的分离和综合利用研究[J]. 林产化学与工业, 2014, 34(6): 61-68.

减压内部沸腾法提取壶瓶枣多糖及其脱色工艺的研究

Extraction of Polysaccharides from Ziziphus Jujube Mill. cv. Hupingzao by Reduced Pressure Boiling Method and Its Decoloration Technology

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们