酶水解过程中纤维素聚合度和结晶度的变化

doi:10.3969/j.issn.0253-2417.2015.05.002

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (5): 5-9.doi: 10.3969/j.issn.0253-2417.2015.05.002

酶水解过程中纤维素聚合度和结晶度的变化

杜敏¹, 李新平¹, 陈立红², 王志杰¹, 李鸿魁¹

1. 陕西科技大学轻工与能源学院, 陕西省造纸技术及特种纸品开发重点实验室, 陕西西安 710021;
2. 陕西科技大学化学与化工学院, 陕西西安 710021

收稿日期:2014-07-30 出版日期:2015-10-25 发布日期:2015-10-24
作者简介:杜敏(1976—),女,浙江嵊州人,讲师,博士,主要从事植物资源化学研究工作
基金资助:
国家自然科学基金面上项目(31370578);陕西省科技厅省级重点实验室项目(2011HBSZS014);陕西科技大学科研启动基金项目(BJ13-21)

Changes of Cellulose Polymerization and Crystallinity Degrees during Enzymatic Hydrolysis

DU Min¹, LI Xin-ping¹, CHEN Li-hong², WANG Zhi-jie¹, LI Hong-kui¹

1. College of Light Industry and Energy, Shaanxi University of Science & Technology;Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, Xi'an 710021, China;
2. College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China

Received:2014-07-30 Online:2015-10-25 Published:2015-10-24

摘要/Abstract

摘要： 分别采用复合纤维素酶Celluclast 1.5L和内切纤维素酶Novozym 476对漂白针叶木纤维进行水解,通过分析酶水解后纤维素聚合度和结晶度的变化情况,研究2种纤维素酶对纤维素聚合度和聚集态结构的影响。结果显示:漂白针叶木纤维经2种纤维素酶分别水解后,随着酶用量的增加,纤维素聚合度都逐渐降低,当Celluclast 1.5 L用量为3.0 FPU/g时,纤维得率为94.34%,纤维素聚合度降至862,较原样(1 164)下降了25.95%;而Novozym 476用量为50.0 U/g时,纤维得率为94.93%,纤维素聚合度降至711,较原样下降了38.92%。由此可见,在得率相同的情况下,经Novozym 476水解的纤维素较经Celluclast 1.5 L水解的纤维素具有更低的聚合度,说明内切葡聚糖酶是导致纤维素聚合度下降的主要因素。随着复合纤维素酶Celluclast 1.5 L用量的增加,纤维素结晶度呈现先增加后降低再增加再降低的M型变化;随着内切纤维素酶Novozym 476用量的增加,纤维素结晶度呈现先降低后增加再降低的变化。

关键词: 纤维素酶, 漂白针叶木纤维, 水解, 聚合度, 结晶度

Abstract: Complex cellulase named Celluclast 1.5L and endo-cellulase named Novozym 476 were applied to hydrolyze the bleached softwood fiber. In the present study, the influences of these two kinds of cellulases on the degree of polymerization (DP) and crystallinity of cellulose were investigated. The results indicated that the DP of cellulose decreased gradually with the increase of the enzyme dosage after hydrolyzed by both cellulases. While the dosage of Celluclast 1.5 L was 3.0 FPU/g, the yield of cellulose was 94.34%. The DP of cellulose decreased to 862 and decreased by 25.95% compared with the control sample (1 164). While the Novozym 476 was in the dosage of 50.0 U/g, the cellulose yield was 94.93%. The DP of cellulose decreased to 711 and decreased by 38.92% compared with the control sample. This showed that in the circumstances of same yield, the cellulose hydrolyzed by Novozym 476 had a lower DP compared with the cellulose hydrolyzed by Celluclast 1.5 L. It meant that endoglucanase was the main factor which led to the decline of DP. With the increase of enzyme dosage of Celluclast 1.5 L, cellulose crystallinity took the changes of M-type, in other words, increased first and then reduced, and finally increased and reduced again. With the increase of enzyme dosage of Novozym 476, the cellulose crystallinity decreased first, and then increased and decreased again.

Key words: cellulase, bleached softwood fiber, hydrolysis, degree of polymerization, crystallinity degree

中图分类号:

TQ35

杜敏, 李新平, 陈立红, 王志杰, 李鸿魁. 酶水解过程中纤维素聚合度和结晶度的变化[J]. 林产化学与工业, 2015, 35(5): 5-9.

DU Min, LI Xin-ping, CHEN Li-hong, WANG Zhi-jie, LI Hong-kui. Changes of Cellulose Polymerization and Crystallinity Degrees during Enzymatic Hydrolysis[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 5-9.

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酶水解过程中纤维素聚合度和结晶度的变化

Changes of Cellulose Polymerization and Crystallinity Degrees during Enzymatic Hydrolysis

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