臭氧预处理对玉米秸秆酶解性能的影响

doi:10.3969/j.issn.0253-2417.2015.06.010

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (6): 58-62.doi: 10.3969/j.issn.0253-2417.2015.06.010

臭氧预处理对玉米秸秆酶解性能的影响

李诚, 王莉, 李永富, 王韧, 陈正行

1. 江南大学粮食发酵工艺与技术国家工程实验室, 江苏无锡 214122;
2. 江南大学食品学院, 江苏无锡 214122

收稿日期:2014-12-25 出版日期:2015-12-25 发布日期:2015-12-28
通讯作者: 陈正行,教授,博士生导师,研究领域为粮食精深加工与利用;E-mail:zxchen_2007@126.com E-mail:zxchen_2007@126.com
作者简介:李诚(1988—),男,山东泰安人,硕士生,主要从事木质纤维素预处理及酶水解研究工作
基金资助:
江南大学校内自主科研重点项目(JUSRP51302A);江苏省科技支撑项目(BE2012428)

Effects of Ozone Pretreatment on the Enzymatic Hydrolysis Efficiency of Corn Stover

LI Cheng, WANG Li, LI Yong-fu, WANG Ren, CHEN Zheng-xing

1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;
2. The School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2014-12-25 Online:2015-12-25 Published:2015-12-28

摘要/Abstract

摘要： 以玉米秸秆为原料,经臭氧预处理后进行酶解制可发酵单糖。研究了不同粒径和含水量对秸秆臭氧处理的影响,确定了最佳的工艺条件,结果表明:秸秆在较小的粒径(<48 μm)和含水率60%条件下臭氧处理效果最好,原料中木质素由15.04%降至2.96%,酶解糖化率从9.17%提高到39.80%。同时探究了最佳条件下臭氧处理时间对处理效果的影响,结果表明:随着处理的进行,木质素降解速率逐渐降低,糖化率在处理75 min时达到40.29%。臭氧消耗量与木质素降解率之间存在较强的线性关系(R²=0.967 9)表明臭氧主要与木质素反应,使木质素降解。臭氧预处理有效提高了秸秆的酶解效率。

关键词: 玉米秸秆, 臭氧处理, 木质素, 酶水解, 线性拟合

Abstract: The corn stover was ozonolyzed and enzymatically hydrolyzed into fermentable sugar.The effects of ozone treatment on corn stovers with different particle sizes and moistures were studied.The optimum particle size and moisture content were determined.The results showed the corn stover with small particle size (< 48 μm) and moisture 60% was preferred for ozonolysis.The lignin content was decreased to 2.96% from 15.04% (raw) and saccharification rate was increased to 39.80% from 9.17% (raw).The effects of reaction time on ozonolysis under the optimum conditions were also investigated.The lignin was gradually degraded with the rising of ozonolysis time, and the saccharification rate was about 40.29% after 75 min.The strong linearity between ozone consumption and lignin degradation rate revealed that ozone mainly reacted with lignin, and resulted in the degradation of the lignin.Ozonolysis treatment could improve the hydrolysis of corn stover effectively.

Key words: corn stover, ozonolysis treatment, lignin, enzymatic hydrolysis, linear fitting

中图分类号:

TQ353.4+2

李诚, 王莉, 李永富, 王韧, 陈正行. 臭氧预处理对玉米秸秆酶解性能的影响[J]. 林产化学与工业, 2015, 35(6): 58-62.

LI Cheng, WANG Li, LI Yong-fu, WANG Ren, CHEN Zheng-xing. Effects of Ozone Pretreatment on the Enzymatic Hydrolysis Efficiency of Corn Stover[J]. Chemistry and Industry of Forest Products, 2015, 35(6): 58-62.

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臭氧预处理对玉米秸秆酶解性能的影响

Effects of Ozone Pretreatment on the Enzymatic Hydrolysis Efficiency of Corn Stover

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