N-(2-亚甲基吡啶)脱氢枞胺希夫碱及其铜配合物与牛血清白蛋白的作用研究

doi:10.3969/j.issn.0253-2417.2016.02.003

林产化学与工业 ›› 2016, Vol. 36 ›› Issue (2): 15-22.doi: 10.3969/j.issn.0253-2417.2016.02.003

N-(2-亚甲基吡啶)脱氢枞胺希夫碱及其铜配合物与牛血清白蛋白的作用研究

王平平¹, 黄志向¹, 高炜琳¹, 张洁¹, 徐武双¹, 张瑜¹, 刘庆波², 龙剑英², 费宝丽¹

1. 南京林业大学化学工程学院, 江苏南京 210037;
2. 南京林业大学理学院, 江苏南京 210037

收稿日期:2015-05-06 出版日期:2016-04-25 发布日期:2016-04-26
通讯作者: 费宝丽,副教授,硕士生导师,主要从事功能配合物和林产配位化学研究;E-mail:hgfbl@njfu.edu.cn。 E-mail:hgfbl@njfu.edu.cn
作者简介:王平平(1992—),女,江苏盐城人,硕士生,主要从事林产配位化学研究;E-mail:1010716132@qq.com
基金资助:
南京林业大学引进高层次留学回国人员科研基金资助项目(163030031);江苏省高校优势学科建设工程资助项目(无编号);江苏省普通高校研究生科研创新计划项目(CXLX13_517)

Binding Ability of N-(2-Methylene Pyridine) Dehydroabietylamine Schiff Base and Its Copper Complex with Bovine Serum Albumin

WANG Ping-ping¹, HUANG Zhi-xiang¹, GAO Wei-lin¹, ZHANG Jie¹, XU Wu-shuang¹, ZHANG Yu¹, LIU Qing-bo², LONG Jian-ying², FEI Bao-li¹

1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
2. College of Science, Nanjing Forestry University, Nanjing 210037, China

Received:2015-05-06 Online:2016-04-25 Published:2016-04-26

摘要/Abstract

摘要： 运用紫外光谱、荧光光谱和圆二色谱研究了具有抗癌活性的N-(2-亚甲基吡啶)脱氢枞胺希夫碱(L)及其铜配合物[Cu(L)₂Br] Br(1)在模拟生理条件(pH值7.2)下与牛血清白蛋白(BSA)的相互作用,分析了它们之间相互作用的机制。结果表明, 1对BSA的荧光猝灭方式为静态猝灭,L为动态猝灭;通过计算得到了不同温度下的结合常数(K_a)、结合位点数(n)及相关的热力学参数(ΔG<0、ΔH>0和ΔS>0),表明L和1与BSA的相互作用力以疏水作用为主,且结合反应是由焓和熵驱动的自发过程。同步荧光光谱分析表明,化合物与BSA的作用使蛋白质的构象发生改变,圆二色谱也证明了化合物能够显著改变BSA的构象,由典型的α-螺旋结构变为扭曲链。

关键词: 脱氢枞胺希夫碱配合物, 牛血清白蛋白, 荧光光谱, 紫外可见光谱, 圆二色谱

Abstract: The binding abilities of anticancer compounds N-(2-methylene pyridine) dehydroabietylamine Schiff bases (L) and its copper complex[Cu(L)₂Br] Br(1) with bovine serum albumin (BSA) under simulated physiological conditions were investigated by UV absorption spectroscopy, fluorescence spectroscopy and circular dichroism (CD). The results indicated that the fluorescence quenching of BSA was a static process by 1, and a dynamic quenching by L. Binding constants(K_a), binding sites (n) and corresponding thermodynamic parameters (ΔG<0,ΔH>0 and ΔS>0) at different temperatures were calculated, respectively. The main binding force between BSA and the compounds was proved to be hydrophobic interaction. The binding reactions were spontaneous processes controlled by enthalpy and free energy. And the analysis of synchronous fluorescence spectroscopy indicated that the binding of compounds changed BSA conformation, and circular dichroism confirmed that the binding interaction obviously modified the secondary structure of BSA from the typical α-helix structure into twisted chain.

Key words: dehydroabietylamine Schiff base complex, bovine serum albumin, fluorescence, UV-vis, circular dichroism

中图分类号:

TQ35

王平平, 黄志向, 高炜琳, 张洁, 徐武双, 张瑜, 刘庆波, 龙剑英, 费宝丽. N-(2-亚甲基吡啶)脱氢枞胺希夫碱及其铜配合物与牛血清白蛋白的作用研究[J]. 林产化学与工业, 2016, 36(2): 15-22.

WANG Ping-ping, HUANG Zhi-xiang, GAO Wei-lin, ZHANG Jie, XU Wu-shuang, ZHANG Yu, LIU Qing-bo, LONG Jian-ying, FEI Bao-li. Binding Ability of N-(2-Methylene Pyridine) Dehydroabietylamine Schiff Base and Its Copper Complex with Bovine Serum Albumin[J]. Chemistry and Industry of Forest Products, 2016, 36(2): 15-22.

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N-(2-亚甲基吡啶)脱氢枞胺希夫碱及其铜配合物与牛血清白蛋白的作用研究

Binding Ability of N-(2-Methylene Pyridine) Dehydroabietylamine Schiff Base and Its Copper Complex with Bovine Serum Albumin

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