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林产化学与工业 ›› 2016, Vol. 36 ›› Issue (3): 16-22.doi: 10.3969/j.issn.0253-2417.2016.03.003

• 研究报告 • 上一篇    下一篇

毛竹竹青和竹黄细胞壁中阿魏酸和对香豆酸定量分析

黄曹兴1, 何娟1, 闵斗勇2, 李鑫1, 杜丽婷3, 勇强1   

  1. 1. 南京林业大学化学工程学院, 江苏 南京 210037;
    2. 南京林业大学轻工科学与工程学院, 江苏 南京 210037;
    3. 南京林业大学现代分析测试中心, 江苏 南京 210037
  • 收稿日期:2015-08-20 出版日期:2016-06-25 发布日期:2016-07-07
  • 通讯作者: 勇强,教授,博士生导师,研究领域:生物质资源生物降解与转化的研究;E-mail:swhx@njfu.com.cn。 E-mail:swhx@njfu.com.cn
  • 作者简介:黄曹兴(1989-),男,福建福清人,博士生,主要从事林产化学加工;E-mail:hcx@njfu.edu.cn
  • 基金资助:
    十二五国家科技支撑计划项目资助(2015BAD15B09);江苏省高校优势学科建设工程资助项目(无编号);国家自然科学基金资助项目(31570561)

Quantitative Determination of Ferulic and p-Coumaric Acids in Cell Wall of Moso Bamboo Green and Bamboo Yellow

HUANG Cao-xing1, HE Juan1, MIN Dou-yong2, LI Xin1, DU Li-ting3, YONG Qiang1   

  1. 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
    3. Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China
  • Received:2015-08-20 Online:2016-06-25 Published:2016-07-07

摘要: 采用两步中度碱水解和两步酸水解法,定量分析毛竹竹青与竹黄细胞壁中阿魏酸和对香豆酸的含量。结果表明(基于绝干物料):竹青细胞壁中含有3.08%阿魏酸和5.91%对香豆酸,其中1.62%阿魏酸和4.64%对香豆酸以酯键形式连接,1.46%阿魏酸和1.27%对香豆酸以醚键形式连接;竹黄细胞壁中阿魏酸和对香豆酸分别为3.11%和5.51%,以酯键和醚键结合的阿魏酸分别为1.71%和1.40%,以酯键和醚键结合的对香豆酸分别为4.34%和1.17%。对碱水解液中溶解的木质素进行二维异核单量子相关核磁共振(2D-HSQC NMR)表征,结果表明:竹青和竹黄碱溶木质素中以酯键存在的阿魏酸和对香豆酸可能酯化在木质素β-O-4醚键的γ位置。

关键词: 竹青, 竹黄, 阿魏酸, 对香豆酸, 定量分析, 2D-HSQC NMR

Abstract: Moderate alkali hydrolysis and acid hydrolysis were applied to quantitatively determine the amount of ferulic and p-coumaric acid in the cell wall of Moso bamboo green and bamboo yellow. The results indicated that 3.07% of ferulic acid and 5.91% of p-coumaric acid were found in the bamboo green cell wall. And 1.61% ferulic acid and 4.64% p-coumaric acid were ester-linked to the bamboo green cell wall components, while 1.46% ferulic acid and 1.27% p-coumaric acid were ether-linked to the cell wall component. For bamboo yellow, there were 3.11% of ferulic acid and 5.51% of p-coumaric acid. The amounts of ester-linked and ether-linked ferulic acid were 1.71% and 1.40%, respectively. And the amounts of ester-linked and ether-linked p-coumaric acid were 4.34% and 1.17%, respectively. Two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance(2D-HSQC NMR) confirmed that the ester-linked ferulic and p-coumaric acid in the alkaline-soluble lignins of bamboo green and bamboo yellow might be esterified in the γ position of β-O-4 aryl ether bonds in the lignin.

Key words: bamboo green, bamboo yellow, ferulic acid, p-coumaric acid, quantitative determination, 2D-HSQC NMR

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