酶辅助提取协同大孔树脂纯化红麸杨果实黄颜木素

doi:10.3969/j.issn.0253-2417.2016.06.016

林产化学与工业 ›› 2016, Vol. 36 ›› Issue (6): 100-106.doi: 10.3969/j.issn.0253-2417.2016.06.016

酶辅助提取协同大孔树脂纯化红麸杨果实黄颜木素

董爱文, 姚姝凤, 赵虹桥, 成江, 伍陵

吉首大学林产化工工程湖南省重点实验室, 湖南张家界 427000

收稿日期:2016-02-17 出版日期:2016-12-25 发布日期:2016-12-23
作者简介:董爱文(1967-),男,湖南桃源人,研究员,硕士,主要从事资源植物的开发与利用;E-mail:daw1999@163.com
基金资助:
黔教合重大专项（字[2012]018号子项301号）；张家界市科技局项目（2015FJ1149）；吉首大学校级科研项目（15JDY018）

Enzyme-assisted Extraction and Macroporous Resin Purification of Fustin from Rhus punjabensis var. Sinica Rehd. Fruit

DONG Ai-wen, YAO Shu-feng, ZHAO Hong-qiao, CHENG Jiang, WU Ling

Forest Products Chemical Engineering Key Laboratory of Hunan Province, Jishou University, Zhangjiajie 427000, China

Received:2016-02-17 Online:2016-12-25 Published:2016-12-23

摘要/Abstract

摘要： 红麸杨果实经纤维素酶处理后，采用体积分数70%乙醇-大孔吸附树脂联用技术对黄颜木素进行提取、纯化，以黄颜木素得率为考察指标，采用正交试验优化了酶辅助提取工艺，并从8种大孔吸附树脂中筛选出对黄颜木素分离纯化最佳的树脂，研究了该树脂对黄颜木素静态、动态吸附与解吸效果。结果表明：酶处理的最佳条件为酶用量120 U/mL，酶解温度55℃，酶解时间60 min，酶解pH值4.5，黄颜木素得率可达1.35%，纯度为14.59%。通过静态吸附试验筛选出最有效的XDA-8树脂用于黄颜木素的纯化，XDA-8分离纯化黄颜木素动态吸附试验的最佳条件为上样质量浓度14.4 g/L，流速80.384 mL/h，洗脱液乙醇体积分数为60%，洗脱液流速80.384 mL/h，解吸率为91.86%，纯化后黄颜木素纯度达到了68.15%。

关键词: 红麸杨果实, 纤维素酶, 黄颜木素, 大孔吸附树脂, HPLC

Abstract: After the Rhus punjabensis var.sinica fruits were disposed by cellulase, the volume fraction of 70% ethanol-macroporous resin combined technique was used to extract and purify fustin. With the fustin yield as study index, the orthogonal experiment was employed to optimize the extraction process. The best resin for fustin separation and purification was screened from eight kinds of macroporous resin to study the effect on the static and dynamic adsorption and desorption of fustin. The results showed that the optimum conditions for the enzyme treatment were enzyme dosage 120 U/mL, enzymolysis temperature 55℃, enzymolysis time 60 min and enzymolysis pH value 4.5, and the fustin yield was up to 1.35% with the purity of 14.59%. Through Static adsorption test, the most effective XDA-8 resin was selected. The dynamic adsorption test showed that the best conditions for XDA-8 separation and purification were sample mass concentration of 14.4 g/L, flow rate of 80.384 mL/h, ethanol volume fraction of 60% and eluent flow rate of 80.384 mL/h. Under these conditions, the desorption rate was 91.86% and after purification the fustin purity could reach 68.15%.

Key words: Rhus punjabensis var.sinica Rehd. fruit, cellulase, dihydrofisetin, macroporous adsorption resin, HPLC

中图分类号:

TQ35

董爱文, 姚姝凤, 赵虹桥, 成江, 伍陵. 酶辅助提取协同大孔树脂纯化红麸杨果实黄颜木素[J]. 林产化学与工业, 2016, 36(6): 100-106.

DONG Ai-wen, YAO Shu-feng, ZHAO Hong-qiao, CHENG Jiang, WU Ling. Enzyme-assisted Extraction and Macroporous Resin Purification of Fustin from Rhus punjabensis var. Sinica Rehd. Fruit[J]. Chemistry and Industry of Forest Products, 2016, 36(6): 100-106.

参考文献

[1] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1999:67-69. Editorial Board of 《Flora Republicae Popularis Sinicac》,CAS.Flora Republicae Popularis Sinicae[M].Beijing:China Science Publishing & Media Ltd,1999:67-69.
[2] 江苏新医学院.中药大辞典[M].上海:上海人民出版社,1997:1817-1819. Jiangsu New Medical College.Chinese Medicine Dictionary[M].Shanghai:Shanghai People's Publishing House,1997:1817-1819.
[3] ZHANG Min,ZHANG Jun-ping,JI Hai-tao,et al.Effect of six flavolloids on proliferation of hepatic stellate cells in vitro[J].Acta Pharmacologica Sinica,2000,21(3):253-256.
[4] 张珉,张俊平,王杰松,等.黄颜木素对HSC-T6细胞增殖和胶原合成的影响[J].第二军医大学学报,1999,20(5):304-305. ZHANG Min,ZHANG Jun-ping,WANG Jie-song,et al.Effects of fisetin on proliferation and collagen synthesis of HSC-T6 cells[J].Academic Journal of Second Military Medical University,1999,20(5):304-305.
[5] 陈虹霞,王成章.漆树黄酮类化合物研究进展[J].天然产物研究与开发,2013,25(12):1752-1758. CHEN Hong-xia,WANG Cheng-zhang.Progress in flavonoid compounds from Rhus verniciflua stokes[J].Natural Product Research and Development,2013,25:1752-1758.
[6] 郭丽冰,孙丽丽,邓琪,等.降香中黄酮类化学成分研究(Ⅱ)[J].中药材,2010,33(6):915-917. GUO Li-bing,SUN Li-li,DENG Qi,et al.Studies on the flavonoids from Lignum dalbergiae Odoriferae(Ⅱ)[J].Journal of Chinese Medicinal Materials,2010,33(6):915-917.
[7] 赵军,崔承彬,蔡兵,等.国产盐肤木属植物的研究进展[J].解放军医学学报,2006,22(1):48-51. ZHAO Jun,CUI Cheng-bin,CAI Bing,et al.The research progress of domestic plants of the genus poison sumac[J].The People's Liberation Army Medical Journals,2006,22(1):48-51.
[8] 陈海霞,肖顺汉.皂角刺抗肿瘤作用有效成分研究进展[J].四川生理科学杂志,2010,32(1):35-37. CHEN Hai-xia,XIAO Shun-han.Study progress on antitumor active ingredients from Spina Gleditsiae[J].Sichuan Physiological Science Magazine,2010,32(1):35-37.
[9] 焦岩,王振宇.响应面法优化纤维素酶辅助提取大果沙棘果渣总黄酮工艺研究[J].林产化学与工业,2010,30(1):85-91. JIAO Yan,WANG Zhen-yu.Optmiization of technology using response surface analysis for extracting flavonoids from seabuckthorn residue assisted by cellulase[J].Chemistry and Industry of Forest Products,2010,30(1):85-91.
[10] 李姝,潘柳谷,吴妮妮,等.生物酶解法提取匙羹藤总皂苷工艺的研究[J].时珍国药,2015,26(7):1616-1617. LI Shu,PAN Liu-gu,WU Ni-ni,et al.Study on extraction process of total saponins of Gymnema sylvestre biological enzyme[J].Lishizheng Medicine and Materia Medica Research,2015,26(7):1616-1617.
[11] 姜贵全,方桂珍,师振鑫,等.超临界CO₂萃取除脂后再经纤维素酶辅助提取落叶松树皮原花青素[J].林产化学与工业,2014,34(2):97-102. JIANG Gui-quan,FANG Gui-zhen,SHI Zhen-xin,et al.Cellulose enzymatic-assisted extraction of proanthocyanidins from the larch bark after degreasing by supercritical CO₂[J].Chemistry and Industry of Forest Products,2014,34(2):97-102.
[12] 徐浩锋,杨华俊,俞岚.中药有效成分现代提取技术研究进展[J].海峡药学,2012,24(1):13-18. XU Hao-feng,YANG Hua-jun,YU Lan.Recent progress in the extraction methods for the active ingredients of Chinese herbal drugs[J].Strait Pharmaceutical Journal,2012,24(1):13-18.
[13] HU Xiu-li,YOU Jing-yan,BAO Chang-li,et al.Determination of total flavonoids in Scutellaria barbata D.Don by dynamic ultrasonic extraction coupled with on-line spectrophotometry[J].Analytica Chimica Acta,2008,610(2):217-223.
[14] 郑红岩,于华忠,刘建兰,等.大孔吸附树脂对蓝莓花色苷的分离工艺[J].林产化学与工业,2014,34(4):59-65. ZHENG Hong-yan,YU Hua-zhong,LIU Jian-lan,et al.Purification of anthocyanin in blueberry by macroporous resin[J].Chemistry and Industry of Forest Products,2014,34(4):59-65.

[1]	赵存异, 应文俊, 史正军, 杨海艳, 郑志锋, 杨静. 羟丙基化碱木质素的制备及其对纤维素酶水解的影响[J]. 林产化学与工业, 2019, 39(2): 109-114.
[2]	董益帆, 刘志佳, 兰天晴. 预处理对蔗渣吸附纤维素酶特性的影响[J]. 林产化学与工业, 2018, 38(2): 75-79.
[3]	王燕云, 吴凯, 杨静. 蒸汽爆破玉米秸秆的分段酶水解[J]. 林产化学与工业, 2016, 36(5): 8-14.
[4]	刘志佳, 董益帆, 兰天晴. 亚硫酸盐预处理对蔗渣酶解效率的影响[J]. 林产化学与工业, 2016, 36(5): 89-94.
[5]	张景强, 李清春, 林鹿. 解结晶对纤维素吸附纤维素酶的影响[J]. 林产化学与工业, 2016, 36(1): 105-111.
[6]	杜敏, 李新平, 陈立红, 王志杰, 李鸿魁. 酶水解过程中纤维素聚合度和结晶度的变化[J]. 林产化学与工业, 2015, 35(5): 5-9.
[7]	黎海龙, 李成国, 秦光辉, 刘健, 甘礼惠, 谢茹胜, 龙敏南. HPLC结合MALDI-TOF-MS分析木聚糖水解产物[J]. 林产化学与工业, 2015, 35(5): 10-14.
[8]	谢普军, 黄立新, 张彩虹, 游凤, 张耀雷. 不同菌种对油橄榄叶发酵饲料的影响[J]. 林产化学与工业, 2015, 35(4): 59-64.
[9]	申莉红, 杨桂花, 陈嘉川. 纤维素酶预处理对杨木APMP浆配抄性能的影响[J]. 林产化学与工业, 2014, 34(6): 105-110.
[10]	何敏超, 许敬亮, 袁振宏, 孔晓英, 张宇, 陈小燕, 梁翠谊. 纤维素酶基因表达研究进展[J]. 林产化学与工业, 2014, 34(5): 169-174.
[11]	郑红岩, 于华忠, 刘建兰, 高梦, 刘同方, 陈雁梅. 大孔吸附树脂对蓝莓花色苷的分离工艺[J]. 林产化学与工业, 2014, 34(4): 59-65.
[12]	王芳芳, 黄峰. 高速及高分辨率原子力显微镜用于研究Trichodermareesei纤维素酶在结晶纤维素上的行为[J]. 林产化学与工业, 2014, 34(3): 130-134.
[13]	姜贵全, 方桂珍, 师振鑫, 张卓睿, 庞久寅. 超临界CO₂萃取除脂后再经纤维素酶辅助提取落叶松树皮原花青素[J]. 林产化学与工业, 2014, 34(2): 97-102.
[14]	李鹏飞;穆彩花;雷福厚;卢建芳;刘祖广;. 松香基大孔吸附树脂对盐酸川芎嗪吸附性能的研究[J]. 林产化学与工业, 2013, 33(1): 74-80.
[15]	何源峰;王成章;陆榕. HPLC-MS法表征坝漆酚类化合物的结构[J]. 林产化学与工业, 2012, 32(6): 47-51.

酶辅助提取协同大孔树脂纯化红麸杨果实黄颜木素

Enzyme-assisted Extraction and Macroporous Resin Purification of Fustin from Rhus punjabensis var. Sinica Rehd. Fruit

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们