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林产化学与工业 ›› 2017, Vol. 37 ›› Issue (2): 135-140.doi: 10.3969/j.issn.0253-2417.2017.02.018

• 研究报告 • 上一篇    下一篇

黑荆树原花色素二聚体的分离制备及抗氧化活性研究

周蒙1,2, 刘功骏1,2, 何凌霄1,2, 王飞1,2   

  1. 1. 南京林业大学 江苏省林业资源高效加工利用协同创新中心, 江苏 南京 210037;
    2. 南京林业大学 化学工程学院;江苏省生物质绿色燃料与化学品重点实验室, 江苏 南京 210037
  • 收稿日期:2016-12-19 出版日期:2017-04-25 发布日期:2017-05-04
  • 通讯作者: 王飞,教授,博士,博士生导师,主要研究领域为生物质能源与生物质化学品;E-mail:hgwf@njfu.edu.cn。 E-mail:hgwf@njfu.edu.cn
  • 作者简介:周蒙(1991-),女,江苏南通人,硕士生,主要从事天然产物分离及其活性方面的研究
  • 基金资助:
    国家重点研发计划(2016YFD0600801);国家林业公益性行业科研专项(201104019)

Preparation and Antioxidant Activity of Proanthocyanidins Dimers from Acacia mearnsii De Willd

ZHOU Meng1,2, LIU Gongjun1,2, HE Lingxiao1,2, WANG Fei1,2   

  1. 1. Jiangsu Co-innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Chemical Engineering, Nanjing Forestry University;Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Received:2016-12-19 Online:2017-04-25 Published:2017-05-04

摘要: 以黑荆树皮为原料,采用超声波辅助提取技术提取原花色素,并利用溶剂萃取法得到低聚原花色素含量较高的乙酸乙酯萃取物;进一步通过葡聚糖凝胶Sephadex LH-20柱和快速蛋白液相色谱(FPLC)分离纯化制备黑荆树原花色素二聚体,并采用HPLC-MS对产物结构进行了鉴定。结果表明:超声波辅助提取原花色素的粗提物得率高达50.21%,其中含原花色素为40.75%,乙酸乙酯萃取物经葡聚糖凝胶Sephadex LH-20柱可得液相纯度约为60%的组分I(得率约为5%);组分I经FPLC分离,水-甲醇为流动相,得到纯度为91.72%的原花色素二聚体(得率约为13%);HPLC-MS分析表明,原花色素二聚体相对分子质量为578,可能是由原花青定的C-4键与原刺槐定的C-8键相连。黑荆树原花色素二聚体对DPPH自由基的清除率分析表明:原花色素二聚体的抗氧化活性总体高于儿茶素以及表没食子儿茶素没食子酸酯(EGCG)标样,当二聚体浓度为60 μmol/L时对DPPH清除率大于80%,高于EGCG(约为68%)和儿茶素(约为50%)。

关键词: 黑荆树皮, 原花色素, 制备, 抗氧化活性

Abstract: Proanthocyanidins were ultrasonic extraction was adopted to extract proanthocyanidins (PC) from Acacia mearnsii De Willd bark powders. Oligomeric proanthocyanidins (OPC) (ethyl acetate extract) were obtained by solvent extraction. Sephadex LH-20 and fast protein liquid chromatography (FPLC) were applied to the separation and purification of PC dimers. The compound was characterized by HPLC-MS. The results indicated that the yield of PC was about 50.21%. The extract was separated by Sephadex LH-20 to obtain the sample Ⅰ with the purity of 60% and the yield of about 5%. The sample Ⅰ was further separated by FPLC with water-methanol as mobile phase, and then the dimer were obtained with the purity of 91.72% and the yield of about 13%. The result of HPLC-MS analysis showed that the relative molecular weight of dimer was 578 and the structure was supposed to be procyandin-(4, 6)-prorobinetinidin. Meanwhile, the results of radical scavenging ability assay indicated that the antioxidant activity of PC dimer was higher than that of catechin and epigallocatechin gallate (EGCG). The scavenging rate of dimer (60 μmol/L) was ≥80% which is higher than that of EGCG (68%) and catechin (50%).

Key words: Acacia mearnsii De Willd bark, proanthocyanidins, preparation, antioxidant activity

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