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林产化学与工业 ›› 2017, Vol. 37 ›› Issue (3): 38-44.doi: 10.3969/j.issn.0253-2417.2017.03.005

• 研究报告 • 上一篇    下一篇

亚氯酸盐预处理杉木细胞壁木质素溶解机理研究

吴博士, 张逊, 杨俊, 许凤   

  1. 生物质化学北京市重点实验室, 北京林业大学, 北京 100083
  • 收稿日期:2016-09-05 出版日期:2017-06-25 发布日期:2017-07-03
  • 通讯作者: 许凤,教授,博士生导师,主要从事生物质高值化利用研究;E-mail:xfx315@bifu.edu.cn E-mail:xfx315@bifu.edu.cn
  • 作者简介:吴博士(1990-),男,山东临沂人,硕士生,主要从事生物质细胞壁超微结构及组分分布研究
  • 基金资助:
    教育部科技计划重点项目(113014A)

Dissolution Mechanism of Lignin in Chinese-fir Cell Walls During Chlorite Pretreatment

WU Boshi, ZHANG Xun, YANG Jun, XU Feng   

  1. Beijing Key Laboratory of Lignocellulose Chemistry, Beijing Forestry University, Beijing 100083, China
  • Received:2016-09-05 Online:2017-06-25 Published:2017-07-03

摘要: 为了探究亚氯酸盐预处理过程中植物细胞壁木质素的区域化学溶解机理,采用紫外-可见光光谱与共聚焦拉曼显微光谱,对杉木管胞细胞壁在不同预处理时间亚细胞区域木质素分布规律及其含量变化进行了研究。结果表明:亚氯酸盐预处理杉木细胞壁木质素的溶解总体上分为4个阶段,木质素少量溶解(Ⅰ),反应溶液缓慢渗透到样品内部(Ⅱ),快速脱除内部大量木质素(Ⅲ),反应平衡阶段(Ⅳ)。尽管杉木细胞壁各层,细胞角隅(CC)、胞间层(CML)及次生壁(SW)都具有木质素的存在,但木质素在细胞壁不同形态区域中的分布具有明显的不均一性,因此在亚氯酸盐预处理过程中细胞壁不同形态学区域木质素的脱除速率不同,大量脱除木质素阶段(8~10 min)时呈现CC > CML > SW的规律。

关键词: 杉木, 亚氯酸盐预处理, 共聚焦拉曼显微光谱, 木质素, 溶解

Abstract: Chlorite pretreatment can selectively remove lignin of cell walls and release cellulose and hemicellulose, which can provide the potential for converting to energy resource, chemical products and biomaterials. In order to study the mechanism of delignification, the dynamic changes of lignin distribution and content in Cunninghamia lanceolata (Lamb.) Hook (Chinese fir) cell walls during chlorite pretreatment were investigated by confocal Raman microscopy (CRM) and ultraviolet spectroscopy. The results showed that the lignin dissolution during the chlorite pretreatment could be clearly divided into four stages:slight removal of lignin (Ⅰ),slow penetration of liquid (Ⅱ), rapid dissolution of lignin (Ⅲ), and the equilibrium phase of delignification (Ⅳ). Although the lignin distributed distinctly in morphological regions,i.e.,cell corner(CC), compound middle lamella(CML), and secondary wall(SW), the distributions were inhomogeneous. Therefore, the removal rates in these areas were different and followed the decreasing order of cell corner > compound middle lamella > secondary wall.

Key words: Chinese fir, chlorite pretreatment, confocal Raman microscopy, lignin, dissolution

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