不同预处理方法对麦草化学组分及其酶解性能的影响

doi:10.3969/j.issn.0253-2417.2017.05.007

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (5): 53-60.doi: 10.3969/j.issn.0253-2417.2017.05.007

不同预处理方法对麦草化学组分及其酶解性能的影响

周静¹, 沈葵忠¹, 房桂干^1,2, 梁龙¹, 田庆文¹, 李漫¹

1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 南京林业大学林业资源高效加工利用协同创新中心, 江苏南京 210037

收稿日期:2017-01-22 出版日期:2017-10-25 发布日期:2017-10-30
通讯作者: 沈葵忠,研究员,硕士生导师,主要从事高得率制浆新技术及生物质高效利用方面的研究工作;E-mail:shenkuizhong@aliyun.com。 E-mail:shenkuizhong@aliyun.com
作者简介:周静(1991-),女,安徽滁州人,硕士生,主要从事生物质化学转化研究工作
基金资助:
中国林科院中央级公益性科研院所基本科研业务费专项资金（CAFYBB2016SY029）；江苏省生物质能源与材料重点实验室项目（JSBEM2014012）

Effects of Different Pretreatment Methods on Chemical Composition and Enzymatic Hydrolysis of Wheat Straw

ZHOU Jing¹, SHEN Kuizhong¹, FANG Guigan^1,2, LIANG Long¹, TIAN Qingwen¹, LI Man¹

1. Institute of Chemical Industry of Forestry Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Collaborative Innovation Center for High Efficient Processing and Utilization of Forestry Resources, Nanjing Forestry University, Nanjing 210037, China

Received:2017-01-22 Online:2017-10-25 Published:2017-10-30

摘要/Abstract

摘要： 以麦草为原料，探讨了高温热水（LHW）、氨水浸渍（SAA）、NaOH和NaOH联合乙醇（NaOH-ET）4种预处理方法对麦草化学组分及其酶水解糖转化率的影响。结果表明：LHW对聚戊糖有较高的去除率，188℃保温40 min处理，木聚糖和阿拉伯聚糖的去除率分别为73.11%和83.10%，但对木质素的去除率较小仅为21.32%。NaOH具有较好的脱木质素和去除灰分作用，麦草经1% NaOH，140℃保温3 h后木质素去除率为72.00%，灰分去除率为75.93%。因木质素在乙醇中有较好的溶解性，相同温度和时间条件下，麦草经1% NaOH与50%乙醇联合预处理，木质素去除率提高至84.11%。XRD检测结果表明，伴随着木质素和半纤维素的溶出，不同方法预处理后物料的结晶度不同程度增加，由原料的28.80%增加至预处理后的31.23%~33.61%。由于木质素和半纤维素的部分脱除，提高了酶对纤维素的可及性，物料经30 FPIU/g （以纤维素质量计，下同）纤维素酶和30 IU/g β-葡萄糖苷酶酶解72 h后，糖转化率明显提高，其中NaOH-ET预处理物料的酶解总糖转化率（以麦草原料为基准）最高达90.05%，是麦草原料的7.5倍。

关键词: 预处理, 高温热水, NaOH-乙醇, 糖转化率

Abstract: Wheat Straw was pretreated by four kinds of pretreatment methods, namely, liquid hot water(LHW), soaking aqueous ammonia(SAA),sodium hydroxide(NaOH) and sodium hydroxide combined with ethanol(NaOH-ET). The effects of pretreatments on chemical composition and enzymatic hydrolysis were investigated. The results showed that LHW pretreatment resulted in the highest level of polypentose reduction. The removal rates of xylan and araban were 73.11% and 83.10% respectively at 188℃ for 40 min, but the delignification rate was only 21.32%. NaOH had a good capability in the removal of lignin and ash. The removal rates of lignin and ash were 72.00% and 75.93% respectively by 1% NaOH at 140℃ for 3 h. Because of the good solubility of lignin in the ethanol, the removal rate of the lignin pretreated by the combination of NaOH with 50% ethanol pretreatment was up to 84.11%. XRD results showed that the crystallization index of four pretreated wheat straw were greater than that of the raw material because of the removal of lignin and hemicellulose; the crystallization index of pretreated samples increased from 28.80% to 31.23%-33.61%. As lignin and hemicellulose were partly removed by pretreatment, the accessibility of cellulose and enzyme inereased. Under the conditions of cellulose enzyme 30 FPIU/g(of cellulose) and β-glucose glycosides enzyme 30 IU/g, enzymatic hydrolysis temperature 50℃, and reaction time 72 h, conversion ratio of total sugar increased obviously. Especially, total sugar conversion rate of NaOH-ET treated wheat straw reached 90.05%, which improved 7.5 times compared with that of raw material.

Key words: pretreatment, liquid hot water, NaOH-C₂H₅OH, sugar conversion rate

中图分类号:

TQ35

周静, 沈葵忠, 房桂干, 梁龙, 田庆文, 李漫. 不同预处理方法对麦草化学组分及其酶解性能的影响[J]. 林产化学与工业, 2017, 37(5): 53-60.

ZHOU Jing, SHEN Kuizhong, FANG Guigan, LIANG Long, TIAN Qingwen, LI Man. Effects of Different Pretreatment Methods on Chemical Composition and Enzymatic Hydrolysis of Wheat Straw[J]. Chemistry and Industry of Forest Products, 2017, 37(5): 53-60.

参考文献

[1] 杨立刚,张军营,王保成. 造纸工业废弃物资源化利用的有效途径[J]. 中国环保产业,2004(7):29-31. YANG L G,ZHANG J Y,WANG B C. Effective approach to reuse resources of paper-making wastes[J]. China Environmental Protection Industry,2004(7):29-31.
[2] MOSIER N,WYMAN C,DALE B,et al. Features of promising technologies for pretreatment of lignocellulosic biomass[J]. Bioresource Technology,2005,96(6):673-686.
[3] 吴义强,李新功,左迎峰,等. 农林剩余物无机人造板研究进展[J]. 林业工程学报,2016,1(1):8-15. WU Y Q,LI X G,ZUO Y F,et al. Research status on the utilization of forest and agricultural biomass in inorganic wood-based panel[J]. Journal of Forestry Engineering,2016,1(1):8-15.
[4] 李楠. 麦草催化转化制备糠醛衍生物研究[D]. 北京:北京林业大学硕士学位论文,2016:1-60. LI N. Catalytical conversion of wheat straw to furfural derivatives[D]. Beijing:Master Degree Thesis of Beijing Forestry University,2016:1-60.
[5] TALEBNIA F,KARAKASHEV D,ANGELIDAKI I. Production of bioethanol from wheat straw:An overview on pretreatment,hydrolysis and fermentation[J]. Bioresource Technology,2010,101(13):4744-4753.
[6] RAGAUSKAS A,WILLIAMS C K,DAVISON B H,et al. The path forward for biofuels and biomaterials[J]. Science,2006,311(5760):484-489.
[7] TAHERZADEH M J,KARIMI K. Pretreatment of lignocellulosic wastes to improve ethanol and biogas production:A review[J]. International Journal of Molecular Sciences,2008,9(9):1621-1651.
[8] 许凤,张逊,周霞,等. 农林生物质预处理过程中细胞壁主要组分溶解机理研究进展[J]. 林业工程学报,2016,1(4):1-9. XU F,ZHANG X,ZHOU X,et al. An investigation of dissolution mechanism of major components in cell walls of agricultural and forest biomass[J]. Journal of Forestry Engineering,2016,1(4):1-9.
[9] LINOJ K,RICHARD C,PABLO A C,et al. Can the same steam pretreatment conditions be used for most softwoods to achieve good enzymatic hydrolysis and sugar yields[J]. Bioresource Technology,2010,101(20):7827-7833.
[10] QING Q,ZHOU L L,GUO Q,et al. A combined sodium phosphate and sodium sulfide pretreatment for enhanced enzymatic digestibility and delignification of corn stover[J]. Bioresource Technology,2016,218:209-216.
[11] LIU H,PANG B,WANG H S,et al. Optimization of alkaline sulfite pretreatment and comparative study with sodium hydroxide pretreatment for improving enzymatic digestibility of corn stover[J]. Journal of Agricultural and Food Chemistry,2015,63(12):3229-3234.
[12] KUMAR P,BARRETT D M,DELWICHE M J,et al. Methods for pretreatment of lignocell-ulosic biomass for efficient hydrolysis and biofuel production[J]. Industrial & Engineering Chemistry Research,2009,48(8):3713-3729.
[13] PÉREZ J A,BALLESTEROS I,BALLESTEROS M,et al. Optimizing liquid hot water pretreatment conditions to enhance sugar recovery from wheat straw for fuel-ethanol production[J]. Fuel,2008,87(17/18):3640-3647.
[14] MOSIER N,HENDRICKSON R,HO N,et al. Optimization of pH controlled liquid hot water pretreatment of corn stover[J]. Bioresource Technology,2005,96(18):1986-1993.
[15] JIN Y C,JAMEEL H,CHANG H M,et al. Green liquor pretreatment of mixed hardwood for ethanol production in a repurposed kraft pulp mill[J]. Journal of Wood Chemistry and Technology,2010,30(1):86-104.
[16] GHOSE T K. Measurement of cellulase activities[J]. Pure and Applied Chemistry,1987,59(2):257-268.
[17] 石淑兰,何福望. 制浆造纸分析与检测[M]. 北京:中国轻工业出版社,2008:25-51. DHI S L,HE F W. Pulp and Paper Analysis and Detection[M]. Beijing:China Light Industry Press,2008:25-51.
[18] SLUITER A,HAMES B,RUIZ R,et al. Determination of Sugars,Byproducts,and Degradation Products in Liquid Fraction Process Samples-Laboratory Analytical Procedure(LAP)[G]. National Renewable Energy Laboratory,2006-08-12.
[19] KLINKE H B,OLSSON L,THOMSEN A B,et al. Potential inhibitors from wet oxidation of wheat straw and their effect on ethanol production of Saccharomyces cerevisiae:Wet oxidation and fermentation by yeast[J]. Biotechnology & Bioengineering,2003,81(6):738-747.
[20] 詹怀宇. 制浆原理与工程[M]. 北京:中国轻工业出版社,2008:38-78. ZHAN H Y. Pulping Principle and Engineering[M]. Beijing:China Light Industry Press,2008:38-78.
[21] GAO Y S,XU J L,ZHANG Y,et al. Effects of different pretreatment methods on chemical composition of sugarcane bagasse and enzymatic hydrolysis[J]. Bioresource Technology,2013,144:396-400.
[22] SILVERSTEIN R A,CHEN Y,SHARMA-SHIVAPPA R R,et al. A compasion of chemical pretreatment methods for improving saccharification of cotton stalkss[J]. Bioresource Technology,2007,98(16):3000-3011.

不同预处理方法对麦草化学组分及其酶解性能的影响

Effects of Different Pretreatment Methods on Chemical Composition and Enzymatic Hydrolysis of Wheat Straw

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