缅甸大果紫檀精油的化学成分及其抑菌活性研究

doi:10.3969/j.issn.0253-2417.2017.06.009

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (6): 65-72.doi: 10.3969/j.issn.0253-2417.2017.06.009

缅甸大果紫檀精油的化学成分及其抑菌活性研究

陈虹霞¹, 康秀棠², 尤龙杰², 王成章^1,3, 叶建中¹

1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 福建省香产品质量检验中心, 福建泉州 362600;
3. 中国林业科学研究院林业新技术研究所, 北京 100091

收稿日期:2017-04-11 出版日期:2017-12-25 发布日期:2018-01-05
通讯作者: 王成章(1966-),研究员,博士,博士生导师,主要从事天然产物研究与利用方面的工作;E-mail:wangczlhs@sina.com E-mail:wangczlhs@sina.com
作者简介:陈虹霞(1983-),女,浙江舟山人,助理研究员,博士,从事天然产物化学方面的研究;E-mail:shirenyahui@126.com
基金资助:
国家国际科技合作专项（2014DFR31300）

Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus

CHEN Hongxia¹, KANG Xiutang², YOU Longjie², WANG Chengzhang^1,3, YE Jianzhong¹

1. Institute of Chemical Industry of Forestry Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Fujian Quality Inspection Center of Incense Products, Quanzhou 362600, China;
3. Research Institute of Forestry New Technology, CAF, Beijing 100091, China

Received:2017-04-11 Online:2017-12-25 Published:2018-01-05

摘要/Abstract

摘要： 采用水蒸气蒸馏（SD）、溶剂热浸提（STE）和亚临界流体萃取（SFE）3种方式提取缅甸大果紫檀精油，并通过GC-MS对3种精油的化学成分进行分析。结果表明：亚临界流体萃取的得率最高（1.95%），水蒸气蒸馏的得率最低（1.03%），溶剂热浸提的精油得率为1.74%。通过GC-MS分析，从水蒸气蒸馏紫檀精油中共检测出38种化合物，主要为β-桉叶醇（69.897%）和桢楠醇（18.055%）；溶剂热浸提紫檀精油中检测出48种化合物，主要为β-桉叶醇（50.907%）、后莫紫檀素（13.322%）、桢楠醇（9.333%）、对叔丁基苯甲醚（2.481%）和匙桉醇（2.285%）；亚临界流体萃取紫檀精油中检测出48种化合物，主要为β-桉叶醇（47.786%）、后莫紫檀素（13.516%）、二甲基-2，3-二氰-2-丁烯二酸（5.429%）、桢楠醇（5.232%）、对叔丁基苯甲醚（2.699%）和匙桉醇（2.636%）。采用抑菌圏法、最小抑菌浓度（MIC）及最低杀菌浓度（MBC）法比较了3种精油的抑菌作用，发现水蒸气蒸馏精油对肺炎克雷伯氏菌、白色念珠菌和枯草芽孢杆菌没有抑制作用，而亚临界流体萃取精油对这3种菌有较好的抑菌效果，其MIC值分别为6.25、25和6.25 g/L。

关键词: 大果紫檀, 精油, 水蒸气蒸馏, 亚临界流体萃取, 抑菌活性

Abstract: The essential oils of Myanmar Pterocarpus macrocarpus Kurz were obtained by using steam distillation(SD), solvent thermal extraction(STE) and subcritical fluid extraction(SFE), with the comparative analysis of the chemical composition of three methods. The results showed that SFE exhibited the highest essential oil yield of 1.95%, SD exhibited the lowest essential oil yield of 1.03%, the yield of STE was 1.74%. About 38 constituents of the essential oil obtained by SD were identified by GC-MS analysis with the main compounds of β-eudesmol(69.897%) and machilol(18.055%). About 48 constituents of the essential oil obtained by STE were identified with the main compounds of β-eudesmol(50.907%), homopterocarpin(13.322%), machilol(9.333%), 1-(1,1-dimethylethyl)-4-methoxy-benzene(2.481%) and espatulenol(2.285%). About 48 constituents of the essential oil obtained by SFE were identified with the main compounds of β-eudesmol(47.786%), homopterocarpin(13.516%), dimethyl 2,3-dicyano-2-butenedioate(5.429%), machilol(5.232%), 1-(1,1-dimethylethyl)-4-methoxy-benzene(2.699%) and espatulenol(2.636%). The antimicrobial activity of the essential oils obtained by threee extraction methods were tested by zone of inhibition test, MIC and MBC assays. The essential oil obtained by SD showed no antimicrobial activity against Klebsiella pneumoniae, Candida albicans and Bacillus subtilis. However, it showed higher antimicrobial activity with the MIC values against the Klebsiella pneumoniae, Candida albicans and Bacillus subtilis of 6.25, 25 and 6.25 g/L, respectively.

Key words: Pterocarpus macrocarpus Kurz, essential oil, steam distillation, subcritical fluid extraction, antibacterial activity

中图分类号:

TQ35

陈虹霞, 康秀棠, 尤龙杰, 王成章, 叶建中. 缅甸大果紫檀精油的化学成分及其抑菌活性研究[J]. 林产化学与工业, 2017, 37(6): 65-72.

CHEN Hongxia, KANG Xiutang, YOU Longjie, WANG Chengzhang, YE Jianzhong. Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus[J]. Chemistry and Industry of Forest Products, 2017, 37(6): 65-72.

参考文献

[1] ROJO J P. Pantropic speciation of Pterocarpus(Leguminosae-Papilionaceae) and the Malesia-Pacific species[J]. Pterocarpus,1977,3(1):19-32.
[2] 周京南. 论中国传统家具珍贵用材的药用价值——以紫檀木、楠木为例[J]. 家具与室内装饰,2012(6):78-83. ZHOU J N. Phamaceutical value of the precious wood for Chinese traditional furniture-Rosewood and nanmu[J]. Furniture & Interior Design,2012(6):78-83.
[3] 刘顺治.黄檀属和紫檀属6种红木化学特性的研究[D].福州:福建农林大学硕士学位论文,2014. LIU S Z. Study on chemical properties of six rosewoods in Dalbergia genus and Pterocarpus genus[D]. Fuzhou:Master Degree Thesis of Fujian Agriculture and Forestry University,2014.
[4] 石江涛,骆嘉言. 气质联用技术在红木挥发油成分分析中的应用[J]. 林业科技开发,2015,29(3):77-80. SHI J T,LUO J Y. Comparison of volatile oil in four rosewood species by gas chromatography-mass spectrometry(GC-MS)[J]. China Forestry Science and Technology,2015,29(3):77-80.
[5] KHAN M R,OMOLOSO A D. Antibacterial activity of Pterocarpus indicus[J]. Fitoterapia,2003,74(6):603-605.
[6] RAGASA C Y. Antimicrobial terpenoids from Pterocarpus indicus[J]. Natural Product Research,2005,19(4):305-309.
[7] 陈林林,米强,辛嘉英. 柑橘皮精油成分分析及抑菌活性研究[J]. 食品科学,2010,31(17):25-28. CHEN L L,MI Q,XIN J Y. Composition analysis and antibacterial activity of the essential oil from citrus peel[J]. Food Science,2010,31(17):25-28.
[8] 董艳鹤. 漆蜡的物化特征及精制加工工艺研究[D]. 北京:中国林业科学研究院硕士学位论文,2011. DONG Y H. Physicochemical properties and refined processing technology of lacquer wax[D]. Beijing:Master Degree Thesis of Chinese Academy of Forestry,2011.
[9] 张育光,侯春,吴新星,等. 亚临界流体萃取崖柏挥发油及其成分分析[J]. 食品工业科技,2015,36(21):210-213. ZHANG Y G,HOU C,WU X X,et al. Extraction of the Thuja sutchuenensis essential oil by sub-critical fluid extraction technology and its component analysis[J]. Science and Technology of Food Industry, 2015,36(21):210-213.
[10] WIDSTEN P,CRUZ C D,FLETCHER G C,et al. Tannins and extracts of fruit byproducts:Antibacterial activity against foodborne bacteria and antioxidant capacity[J]. Journal of Agricultural & Food Chemistry,2014,62(46):11146-11156.
[11] IVANOVIC J,DIMITRIJEVIC-BRANKOVIC S,MISIC D,et al. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds[J]. Journal of Functional Foods,2013,5(1):416-423.
[12] AHANGARI B,SARGOLZAEI J. Extraction of pomegranate seed oil using subcritical propane and supercritical carbon dioxide[J]. Theoretical Foundations of Chemical Engineering, 2012,46(3):259-265.
[13] 苗建银,车科,陈雪香,等. 亚临界流体萃取油茶籽油的抗氧化活性研究[J]. 林产化学与工业,2012,32(4):67-71. MIAO J Y,CHE K,CHEN X X,et al. Antioxidant activity of camellia oil extracted by the sub-critical fluid[J].Chemistry and Industry of Forest Products,2012,32(4):67-71.
[14] 王金华,薛宝云,梁爱华,等. 苍术有效成分β-桉叶醇对小鼠小肠推进功能的影响[J].中国药学杂志,2002,37(4):266-268. WANG J H,XUE B Y,LIANG A H,et al. Effects of β-eudesmol,an active constituent from Rhizoma atractylodis on small intestine movement in rats[J].Chinese Pharmaceutical Journal,2002,37(4):266-268.
[15] 周德文,周立勇. 术类的药理和药效[J]. 国外医药-植物药分册,1996,11(3):120-122. ZHOU D W,ZHOU L Y. Pharmacological and pharmacodynamics of Rhizoma atractylodis[J]. Drugs & Clinic,1996,11(3):120-122.
[16] 李伟东,阚毓铭,洪敏,等. 后莫紫檀素、美迪紫檀素对人肝癌细胞抑制作用的研究[J]. 沈阳药科大学学报,2001,18(3):211-212. LI W D,KAN Y M,HONG M,et al. Anti hepatocellular carcinoma cell activity of homopterocarpin and medicarpin[J]. Journal of Shenyang Pharmaceutical University,2001,18(3):211-212.
[17] 阚毓铭,朱荃,陈龙,等. 从刺果甘草中分离出的后莫紫檀素对HEp-2细胞的作用[J]. 中国药学杂志,1994,29(10):608-609. KAN Y M,ZHU Q,CHEN L,et al. Anti HEp-2 cell activity of the homopterocarpin isolate from Glycyrrhiza pallidiflora Maxim[J]. Chinese Pharmaceutical Journal,1994,29(10):608-609.
[18] OLALEYE M T,AKINMOLADUN A C,CROWN O O,et al. Homopterocarpin contributes to the restoration of gastric homeostasis by Pterocarpus erinaceus,following indomethacin intoxication in rats[J]. Asian Pacific Journal of Tropical Medicine,2013,6(3):200-204.
[19] 王宏年,江志利,冯俊涛,等. 7种植物精油对番茄灰霉病的抑制效果[J]. 植物保护,2007,33(5):111-114. WANG H N,JIANG Z L,FENG J T,et al.Inhibitory effects of seven essential oils against Botrytis cinerea[J]. Plant Protection. 2007,33(5):111-114.
[20] ELISABETSKY E,BRUM L F,SOUZA D O. Anticonvulsant properties of linalool in glutamate-related seizure models[J]. Phytomedicine International Journal of Phytotherapy & Phytopharmacology,1999,6(2):107-113.
[21] PERUMALSAMY H,KIM N J,AHN Y J. Larvicidal activity of compounds isolated from Asarum heterotropoides against Culex pipiens pallens,Aedes aegypti,and Ochlerotatus togoi(Diptera:Culicidae)[J]. Journal of Medical Entomology,2009,46(6):1420-1423.

缅甸大果紫檀精油的化学成分及其抑菌活性研究

Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus

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