[1] KIM W C,KIM J Y,KO J H,et al. Transcription factor MYB46 is an obligate component of the transcriptional regulatory complex for functional expression of secondary wall-associated cellulose synthases in Arabidopsis thaliana[J]. Journal of Plant Physiology,2013,170(15):1374-1378. [2] YEOMAN C J,HAN Y J,DODD D,et al. Thermostable enzymes as biocatalysts in the biofuel industry[J]. Advances in Applied Microbiology,2010,70:1-55. [3] ALONSO D M,HAKIM S H,ZHOU S,et al. Increasing the revenue from lignocellulosic biomass:Maximizing feedstock utilization[J]. Science Advances,2017,3(5):1-7[2017-08-02]. https://www.researchgate.net/publication/317044934.DOI:10.1126/sciadv.1603301. [4] MERINO S T,CHERRY J. Progress and challenges in enzyme development for biomass utilization[J]. Advances in Biochemical Engineering/Biotech-nology,2007,108:95-120. [5] WILSON D B. Cellulases and biofuels[J]. Current Opinion in Biotechnology,2009,20(3):295-299. [6] THONGEKKAEW J,IKEDA H,MASAKI K,et al. Fusion of cellulose binding domain from Trichoderma reesei CBHI to Cryptococcus,sp. S-2 cellulase enhances its binding affinity and its cellulolytic activity to insoluble cellulosic substrates[J]. Enzyme and Microbial Technology,2013,52(4/5):241-246. [7] YANG S J,KATAEVA I,HAMILTON-BREHM S D,et al. Efficient degradation of lignocellulosic plant biomass,without pretreatment,by the thermophilic anaerobe "Anaerocellum thermophilum" DSM 6725[J]. Applied and Environmental Microbiology,2009,75(14):4762-4769. [8] PHUONGAN D,IRINA K,YANG S J,et al. Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725[J]. Nucleic Acids Research,2011,39(8):3240-3254. [9] BRUNECKY R,ALAHUHTA M,XU Q,et al. Revealing nature's cellulase diversity:The digestion mechanism of Caldicellulosiruptor bescii CelA[J]. Science,2013,342(6165):1513-1516. [10] YOUNG J,CHUNG D,BOMBLE Y J,et al. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass[J]. Biotechnology for Biofuels,2014,7:1-8[2017-08-02]. http://www.biotechnologyforbio-fuels.com/content/7/1/142.DOI:10.1186/s13068-014-0142-6. [11] TELKE A A,GHATGE S S,KANG S H,et al. Construction and characterization of chimeric cellulases with enhanced catalytic activity towards insoluble cellulosic substrates[J]. Bioresource Technology,2012,112(1):10-17. [12] TE'O V S J,SAUL D J,BERGQUIST P L. CelA,another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum[J]. Applied Microbiology and Biotechnology,1995,43(2):291-296. [13] ZVERLOV V,MAHR S,RIEDEL K,et al. Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile ‘Anaerocellum thermophilum’ with separate glycosyl hydrolase family 9 and 48 catalytic domains[J]. Microbiology,1998,144(2):457-465. [14] ZURAWSKI J V,CONWAY J M,LEE L L,et al. Comparative analysis of extremely thermophilic Caldicellulosiruptor species reveals common and unique cellular strategies for plant biomass utilization[J]. Applied and Environmental Microbiology,2015,81(20):7159-7170. [15] PARK J I,KENT M S,DATTA S,et al. Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus[J]. Bioresource Technology,2011,102(10):5988-5994. [16] YE L B,SU X Y,SCHMITZ G E,et al. Molecular and biochemical analyses of the GH44 module of CbMan5B/Cel44A,a bifunctional enzyme from the hyperthermophilic bacterium Caldicellulosiruptor bescii[J]. Applied and Environmental Microbiology,2012,78(19):7048-7059. [17] YI Z L,SU X Y,REVINDRAN V,et al. Molecular and biochemical analyses of CbCel9A/Cel48A,a highly secreted multi-modular cellulase by Caldicellulosiruptor bescii during growth on crystalline cellulose[J]. PLOS One,2013,8(12):1-15.[2017-08-02].https://ww.researchgate.net/publi-cation/259396340.DOI:10.1371/journal.pone.0084172. [18] 白挨玺. 细菌Caldicellulosiruptor bescii嗜热纤维素酶系功能及协同作用研究[D]. 吉林:吉林大学博士学位论文,2012. BAI A X. Functional and synergistic studies of thermophilic cellulases from Caldicellulosiruptor bescii[D]. Jilin:Doctoral Dissertation of Jilin University,2012. [19] CHIRIAC A I,CADENA E M,VIDAL T,et al. Engineering a family 9 processive endoglucanase from Paenibacillus barcinonensis displaying a novel architecture[J]. Appl Microbiol Biotechel, 2010, 86(4):1125-1134. [20] SU X Y,MACKIE R I,CANN I K O. Biochemical and mutational analyses of a multidomain cellulase/mannanase from Caldicellulosiruptor bescii[J]. Applied and Environmental Microbiology,2012,78(7):2230-2240. [21] KARITA S,SAKKA K,OHMIYA K. Cellulose-binding domains confer an enhanced activity against insoluble cellulose to Ruminococcusalbus,endoglucanase IV[J]. Journal of Fermentation and Bioengineering,1996,81(6):553-556. [22] LEMOS M A,MRM T J,MOTA M,et al. The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei[J]. Enzyme and Microbial Technology,2003,32(1):35-40. [23] GUNDLLAPALLI S B,PRETORIUS I S,CORDERO OTERO R R. Effect of the cellulose-binding domain on the catalytic activity of a glucosidase from Saccharomycopsis fibuligera[J]. Journal of Industrial Microbiology & Biotechnology,2007,34:413-421. [24] MENG D D,YING Y,CHEN X H,et al. Distinct roles for carbohydrate-binding modules of glycoside hydrolase 10(GH10) and GH11 xylanases from Caldicellulosiruptor sp. strain F32 in thermostability and catalytic efficiency[J]. Applied and Environmental Microbiology,2015,81(6):2006-2014. [25] KATAEVA I A,YANG S J,DAM P,et al. Genome sequence of the anaerobic,thermophilic,and cellulolytic bacterium "Anaerocellum thermophilum" DSM 6725[J]. Journal of Bacteriology,2009,191(11):3760-3761. [26] SAKON J,IRWIN D,WILSON D B,et al. Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca[J]. Nature Structural & Molecular Biology,1997,4(10):810-818. [27] HONDA Y,SHIMAYA N,ISHISAKI K,et al. Elucidation of exo-β-D-glucosaminidase activity of a family 9 glycoside hydrolase (PBPRA0520) from Photobacterium profundum SS9[J]. Glycobiology,2011,21(4):503-511. [28] PARSIEGLA G,JUY M,REVERBELLEROY C,et al. The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 A resolution[J]. EMBO Journal,1998,17(19):5551-5562. [29] PARSIEGLA G,REVERBELLEROY C,TARDIF C,et al. Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action[J]. Biochemistry,2000,39(37):11238-11246. [30] GUIMARAES B G,SOUCHON H,LYTLE B L,et al. The crystal structure and catalytic mechanism of cellobiohydrolase CelS,the major enzymatic component of the Clostridium thermocellum cellulosome[J]. Journal of Molecular Biology,2002,320(3):587-596. [31] PARSIEGLA G,REVERBEL C,TARDIF C,et al. Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocello oligosaccharides give rise to a new view of the substrate pathway during processive action[J]. Journal of Molecular Biology,2008,375(2):499-510. [32] TING C L,MAKAROW D E,WANG Z G. A kinetic model for the enzymatic action of cellulase[J]. Journal of Physical Chemistry B,2009,113(14):4970-4977. [33] BATISTA P R,COSTA M G,PASCUTTI P G,et al. High temperatures enhance cooperative motions between CBM and catalytic domains of a thermostable cellulase:Mechanism insights from essential dynamics[J]. Physical Chemistry Chemical Physics,2011,13(30):13709-13720. [34] FAN Z M,WAGSCHAL K,LEE C C,et al. The construction and characterization of two xylan-degrading chimeric enzymes[J]. Biotechnology and Bioengineering,2009,102(3):684-692. [35] LU P,FENG M G,LI W F,et al. Construction and characterization of a bifunctional fusion enzyme of Bacillus-sourced β-glucanase and xylanase expressed in Escherichia[J]. FEMS Microbiology Letters,2006,261(2):224-230. [36] SU Y H,JIN S L,CHO K M,et al. Assembling a novel bifunctional cellulase-xylanase from Thermotoga maritima,by end-to-end fusion[J]. Biotechnology Letters,2006,28(22):1857-1862. [37] LEE H L,CHANG C K,TENG K H,et al. Construction and characterization of different fusion proteins between cellulases and β-glucosidase to improve glucose production and thermostability[J]. Bioresource Technology,2011,102(4):3973-3976. [38] HONG S Y,LEE J S,CHO K M,et al. Construction of the bifunctional enzyme cellulase-β-glucosidase from the hyperthermophilic bacterium Thermotoga maritima[J]. Biotechnology Letters,2007,29(6):931-936. [39] ELLEUCHE S. Bringing functions together with fusion enzymes from nature's inventions to biotechnological applications[J]. Applied Microbiology and Biotechnology,2015,99(4):1545-1556. [40] LU P,FENG M G. Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers[J]. Applied Microbiology and Biotechnology,2008,79(4):579-587. [41] KIM H M,JUNG S,LEE K H,et al. Improving lignocellulose degradation using xylanase-cellulase fusion protein with a glycine-serine linker[J]. International Journal of Biological Macromolecules,2015,73:215-221. [42] ADLAKHA N,SAWANT S,ANIL A,et al. Specific fusion of β-1,4-endoglucanase and β-1,4-glucosidase enhances cellulolytic activity and helps in channeling of intermediates[J]. Applied and Environmental Microbiology,2012,78(20):7447-7454. [43] ADLAKHA N,RAJAGOPAL R,KUMAR S,et al. Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium[J]. Applied and Environmental Microbiology,2011,77(14):4859-4866. [44] KURNIASIH S D,ALFI A,NATALIA D,et al. Construction of individual,fused,and co-expressed proteins of endoglucanase and β-glucosidase for hydrolyzing sugarcane bagasse[J]. Microbiological Research,2014,169(9/10):725-732. [45] RIEDEL K,BRONNENMEIER K. Intramolecular synergism in an engineered exo-endo-1,4-β-glucanase fusion protein[J]. Molecular Microbiology,1998,28(4):767-775. [46] DUEDU K O,FRENCH C E. Characterization of a Cellulomonas fimi exoglucanase/xylanase-endoglucanase gene fusion which improves microbial degradation of cellulosic biomass[J]. Enzyme and Microbial Technology,2016,93/94:113-121. [47] AN J M,KIM Y K,LIM W J,et al. Evaluation of a novel bifunctional xylanase-cellulase constructed by gene fusion[J]. Enzyme and Microbial Technology,2005,36(7):989-995. [48] RIZK M,ANTRANIKIAN G,ELLEUCHE S. End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes[J]. Biochemical and Biophysical Research Communications,2012,428(1):1-5. [49] KIM S K,MIN W K,PARK Y C,et al. Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme Ⅱ[J]. Enzyme and Microbial Technology,2015,79/80:49-54. [50] KIM S K,CHUNG D,HIMMEL M E,et al. Engineering the N-terminal end of CelA results in improved performance and growth of Caldicellulosiruptor bescii on crystalline cellulose[J]. Biotechnology and Bioengineering,2016,114(5):945-950. [51] 张馨月. 新型双功能糖苷酶的表达纯化及功能研究[D]. 苏州:苏州大学硕士学位论文,2013. ZHANG X Y. Expression,purification and bioactivity characterization of a novel bifunctional edno F2/PNGase F glycoside hydrolase[D]. Suzhou:Master Degree Thesis of Suzhou University,2013. [52] 孙军涛. 耐热β-1,3-1,4-葡聚糖酶的构建及其酶学性质研究[D]. 无锡:江南大学博士学位论文,2012. SUN J T. Construction of thermostable β-1,3-1,4-glucanase and its enzymatic properties[D]. Wuxi:Doctoral Dissertation of Jiangnan University,2012. [53] 刘靖,夏文水. 焦碳酸二乙酯及碳二亚胺对纤维素酶-壳聚糖酶双功能酶的修饰作用[J]. 食品科学,2008,29(8):460-463. LIU J,XIA W S. Modification of cellulase-chitosanase bifunctional enzyme by diethyl pyrocarbonate and carbodiimide[J]. Food Science,2008,29(8):460-463. [54] 刘萍. 绿色木霉产双功能酶的结构与功能研究[D]. 无锡:江南大学博士学位论文,2009. LIU P. Study on Structure and function of bifunctional cellulase-chitosanase from Trichoderma viride[D]. Wuxi:Doctoral Dissertation of Jiangnan University,2009. [55] 彭静静. 多功能半纤维素酶的构建及其酶解应用分析[J]. 湖北农业科学,2014,53(16):3933-3935. PENG J J. Constructing trifunctional hemicellulase and degradation of arabinoxylan[J]. Hubei Agricultural Sciences,2014,53(16):3933-3935. [56] KHALILI G K,AKBARI N K,SHAHBANI Z H. Development of a bifunctional xylanase-cellulase chimera with enhanced activity on rice and barley straws using a modular xylanase and an endoglucanase procured from camel rumen metagenome[J]. Applied Microbiology and Biotechnology,2017,101(18):6929-6939. [57] CHUNG D H,CHA M S,GUSS A M,et al. Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii[J]. Proceedings of the National Academy of Sciences of the United States of America,2014,111(24):8931-8936. |