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林产化学与工业 ›› 2018, Vol. 38 ›› Issue (2): 11-20.doi: 10.3969/j.issn.0253-2417.2018.02.002

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双催化域糖苷水解酶研究进展

夏继林1, 谭中标1,2, 朱小燕1,2, 时号1,2, 李相前1,2   

  1. 1. 淮阴工学院 生命科学与食品工程学院, 江苏 淮安 223003;
    2. 江苏省生物质转化与过程集成工程实验室, 江苏 淮安 223003
  • 收稿日期:2017-09-02 出版日期:2018-04-25 发布日期:2018-04-28
  • 通讯作者: 李相前,教授,硕士生导师,从事生物技术研究;E-mail:lixq2002@126.com。 E-mail:lixq2002@126.com
  • 作者简介:夏继林(1992-),男,江苏淮安人,硕士生,从事双催化域糖苷水解酶的重构研究;E-mail:2032712058@qq.com
  • 基金资助:
    国家自然科学基金资助项目(21576110,21706089)

Research Advances on Glycoside Hydrolases with Two Catalytic Domains

XIA Jilin1, TAN Zhongbiao1,2, ZHU Xiaoyan1,2, SHI Hao1,2, LI Xiangqian1,2   

  1. 1. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China;
    2. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huai'an 223003, China
  • Received:2017-09-02 Online:2018-04-25 Published:2018-04-28

摘要: 综述了双催化域糖苷水解酶的天然结构域解析、人工构建、修饰及其应用等方面的研究进展,突出对双催化域糖苷水解酶在提高热稳定性和催化活性等方面的挖掘,探讨了双催化域糖苷水解酶的降解机制,最后对双催化域糖苷水解酶的研究方向进行了展望。

关键词: 双催化域糖苷水解酶, 结构域解析, 人工构建, 结构修饰

Abstract: This review presented the reaserch progresses in the natural domains analysis, artificial construction, modification, and application of glycoside hydrolases with two catalytic domains. The study of glycoside hydrolases with two catalytic domains on improving thermostability and catalytic activity were highlighted. In addition, the catalytic mechanism of glycoside hydrolases with two catalytic domains was explored, and also the research directions of glycoside hydrolases with two catalytic domains were prospected.

Key words: glycoside hydrolase with two catalytic domains, domain analysis, artificial construction, structure modification

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