纤维素纳米晶荧光探针的制备及光学性能

doi:10.3969/j.issn.0253-2417.2018.06.007

林产化学与工业 ›› 2018, Vol. 38 ›› Issue (6): 51-58.doi: 10.3969/j.issn.0253-2417.2018.06.007

纤维素纳米晶荧光探针的制备及光学性能

宋如愿, 赵璐洋, 夏子华, 吴伟兵, 景宜, 戴红旗

南京林业大学江苏省林业资源高效加工利用协同创新中心;江苏省制浆造纸科学与技术重点实验室, 江苏南京 210037

收稿日期:2018-07-02 出版日期:2018-12-25 发布日期:2018-12-27
通讯作者: 吴伟兵,教授,博士,主要从事纳米纤维素功能材料的研究;E-mail:wbwu@njfu.edu.cn。 E-mail:wbwu@njfu.edu.cn
作者简介:宋如愿(1994-),女,江苏连云港人,硕士生,主要从事纳米纤维素功能材料的研究工作
基金资助:
江苏省自然科学基金资助项目（BK20171450）；国家重点研发计划子课题（2017YFD0601000）

Preparation and Optical Properties of Fluorescent Probes Based on Cellulose Nanocrystals

SONG Ruyuan, ZHAO Luyang, XIA Zihua, WU Weibing, JING Yi, DAI Hongqi

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources;Jiangsu Provincial Key Lab of Pulp and Papermaking Science and Technology, Nanjing Forestry University, Nanjing 210037, China

Received:2018-07-02 Online:2018-12-25 Published:2018-12-27

摘要/Abstract

摘要： 将羧基化纤维素纳米晶（C-CNC）与4-（（2-氨基乙基）氨基）-9-甲基-1，8-萘二甲酰亚胺（AANI）染料共价键结合制得荧光纤维素纳米晶（F-CNC），并采用透射电子显微镜（TEM）、X射线衍射（XRD）和红外光谱（FT-IR）等对其进行了表征，进一步分析了F-CNC的光学性质和荧光标记成像性能。表征结果表明：AANI成功连接到了C-CNC表面，CNC基底提高了染料的亲水性，AANI在H₂O中的最大溶解度为0.8 mmol/L，而制备的F-CNC母液中染料浓度为1.2 mmol/L。F-CNC在水溶液中分散稳定，且呈现良好的亮黄色荧光发射性能；同时F-CNC保留了C-CNC的棒状形态、尺寸变化不大，结晶度及晶体结构也没有明显变化。C-CNC与F-CNC的结晶度分别为61.2%和67.7%。由于溶剂极性降低会压缩CNC双电层，造成CNC呈现一定程度聚集状态，这使F-CNC相较于AANI表现出对溶剂极性更高的荧光敏感性。荧光标记成像结果表明：当F-CNC质量浓度为0、3.375×10^-4和6.75×10^-4 g/L时，孵育10 h后细胞的存活率分别为49.08%、44.21%和40.71%，同时F-CNC对Hela细胞具有良好的荧光标记成像性能，且对细胞毒性不大。

关键词: 纤维素纳米晶, 荧光探针, 生物成像, 溶剂极性, 双电层

Abstract: Multifunctional fluorescent cellulose nanocrystals (F-CNC) was prepared by the covalently bonded carboxylated cellulose nanocrystals (C-CNC) with 4-((2-aminoethyl)amino)-9-methyl-1,8-naphthalimide (AANI). Then, F-CNC was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and infrared spectroscopy (FT-IR), respectively. The optical properties and fluorescence-labeled imaging properties of F-CNC were further analyzed. The characterization results showed that AANI was successfully attached to the surface of C-CNC and the CNC substrate improved the hydrophilicity of the dye. The maximum solubility of AANI in H₂O was 0.8 mmol/L, while the concentration of dye in the prepared F-CNC mother liquor was 1.2 mmol/L. F-CNC was stable in aqueous solution and exhibited a good bright yellow fluorescence emission property. At the same time, F-CNC retained the rod shape of C-CNC and had little change in size. The crystallinity and crystal structure didn't changed significantly. The crystallinities of C-CNC and F-CNC were 61.2% and 67.7%, respectively. The decrease of the solvent polarity compressed the double layer of the F-CNC and caused the aggregation of the F-CNC to some extent, resulting in higher fluorescence sensitivity of F-CNC to solvent polarity than that of AANI. The results of fluorescent labeling showed that when the mass concentrations of F-CNC were 0, 3.375×10^-4 and 6.75×10^-4 g/L, the survival rates of cells after incubated for 10 h were 49.08%, 44.21% and 40.71%, respectively. F-CNC had good fluorescent-labeled imaging performance for Hela cells, and had low toxicity to cells.

Key words: cellulose nanocrystals, fluorescent probe, bioimaging, solvent polarity, double layer

中图分类号:

TQ35

宋如愿, 赵璐洋, 夏子华, 吴伟兵, 景宜, 戴红旗. 纤维素纳米晶荧光探针的制备及光学性能[J]. 林产化学与工业, 2018, 38(6): 51-58.

SONG Ruyuan, ZHAO Luyang, XIA Zihua, WU Weibing, JING Yi, DAI Hongqi. Preparation and Optical Properties of Fluorescent Probes Based on Cellulose Nanocrystals[J]. Chemistry and Industry of Forest Products, 2018, 38(6): 51-58.

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纤维素纳米晶荧光探针的制备及光学性能

Preparation and Optical Properties of Fluorescent Probes Based on Cellulose Nanocrystals

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