青龙衣中胡桃醌的提取及其抑菌活性研究

doi:10.3969/j.issn.0253-2417.2019.05.013

林产化学与工业 ›› 2019, Vol. 39 ›› Issue (5): 95-100.doi: 10.3969/j.issn.0253-2417.2019.05.013

青龙衣中胡桃醌的提取及其抑菌活性研究

李文君¹, 王成章¹, 刘辰婧²

1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业和草原局林产化学工程重点实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 南京林业大学轻工与食品学院, 江苏南京 210037

收稿日期:2019-02-18 出版日期:2019-10-25 发布日期:2019-11-01
通讯作者: 王成章,研究员,博士生导师,研究领域为天然产物化学与利用;E-mail:wangczlhs@sina.com E-mail:wangczlhs@sina.com
作者简介:李文君(1989-),女,河南周口人,助理研究员,博士生,主要从事木本植物效用成分的研究
基金资助:
江苏省生物质能源与材料重点实验室基本科研业务费项目（JSBEM-S-201810）；中国林科院中央级公益性科研院所基本科研业务费专项资金（CAFYBB2017ZA004-2）

Extraction and Antimicrobial Activity of Juglone from Pericarpium Juglandis

LI Wenjun¹, WANG Chengzhang¹, LIU Chengjing²

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China

Received:2019-02-18 Online:2019-10-25 Published:2019-11-01

摘要/Abstract

摘要： 以青龙衣为原料，采用超声波辅助提取青龙衣中胡桃醌，通过单因素和正交试验优化得到最佳提取工艺条件；并以甲醇和商用YZN-Y500纳米银抗菌溶液作对照，考察最佳提取工艺条件下得到的质量浓度分别为0.20、0.02、0.01、0.005和0.002 5 g/L的青龙衣提取物样品溶液，对枯草芽孢杆菌、肺炎链球菌、金黄色葡萄球菌和表皮葡萄球菌的抑菌活性。研究结果表明：以甲醇为浸提溶剂，浸泡时间6 h，液料比5：1（mL：g），超声波作用时间35 min的最佳提取条件下胡桃醌得率0.246%，浸膏得率13.88%。青龙衣提取物溶液具有较强的抑菌活性，且溶液浓度越高，抑菌效果越明显；质量浓度为0.20、0.02 g/L的青龙衣提取物样品溶液对4种菌的抑菌圈直径可达15~25 mm，抑菌效果强于0.01g/L的商用纳米银溶液。

关键词: 青龙衣, 胡桃醌, 超声波辅助, 纳米银溶液, 抑菌活性

Abstract: Juglone was extracted from pericarpium juglandis by ultrasound-assisted method. The optimal conditions of extracting juglone were obtained by single experiment and orthogonal test as follows:soaking time was 6 h using methanol as solvent, ratio of liquid to material was 5:1(mL:g), ultrasonic time was 35 min, and the yield of juglone was 0.246% and the yield of extract was 13.88%. The antibacterial activities of extract on Bacillus subtilis, Streptococcus pneumoniae, Staphylococcus aureus and S. epidermidis of juglone was investigated by mass concentration of 0.002 5-0.20 g/L, compared with commercial YZN-Y500 nano-silver. The results showed that the optimum extraction conditions was as follows:The antibacterial effect of pericarpium juglandis extract on the mass concentrations of 0.20 g/L and 0.02 g/L was stronger than that of commercial silver nanoparticles.

Key words: pericarpium juglandis, nutcrone, ultrasound assisted, nano-silver solution, antibacterial activity

中图分类号:

TQ35

李文君, 王成章, 刘辰婧. 青龙衣中胡桃醌的提取及其抑菌活性研究[J]. 林产化学与工业, 2019, 39(5): 95-100.

LI Wenjun, WANG Chengzhang, LIU Chengjing. Extraction and Antimicrobial Activity of Juglone from Pericarpium Juglandis[J]. Chemistry and Industry of Forest Products, 2019, 39(5): 95-100.

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青龙衣中胡桃醌的提取及其抑菌活性研究

Extraction and Antimicrobial Activity of Juglone from Pericarpium Juglandis

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