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林产化学与工业 ›› 2019, Vol. 39 ›› Issue (6): 59-67.doi: 10.3969/j.issn.0253-2417.2019.06.008

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五味子果肉和种子精油的化学成分与生物活性对比研究

王洁1,路娇娇1,杜涵1,张萌1,刘盼盼1,陈小强2,*()   

  1. 1. 东北林业大学 生命科学学院, 黑龙江 哈尔滨 150040
    2. 东北林业大学 森林植物生态学教育部重点实验室, 黑龙江 哈尔滨 150040
  • 收稿日期:2019-05-31 出版日期:2019-12-28 发布日期:2019-12-21
  • 通讯作者: 陈小强 E-mail:xiaoqiang_chen@nefu.edu.cn
  • 作者简介:王洁(1997-),女,山东烟台人,本科生,研究方向为生物技术
  • 基金资助:
    中央高校基本科研业务费专项资金项目(2572015CB18);东北林业大学大学生创新训练项目(201810225502);黑龙江省自然科学基金项目(LH2019C006)

Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities

Jie WANG1,Jiaojiao LU1,Han DU1,Meng ZHANG1,Panpan LIU1,Xiaoqiang CHEN2,*()   

  1. 1. College of Life Science, Northeast Forestry University, Harbin 150040, China
    2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
  • Received:2019-05-31 Online:2019-12-28 Published:2019-12-21
  • Contact: Xiaoqiang CHEN E-mail:xiaoqiang_chen@nefu.edu.cn
  • Supported by:
    中央高校基本科研业务费专项资金项目(2572015CB18);东北林业大学大学生创新训练项目(201810225502);黑龙江省自然科学基金项目(LH2019C006)

摘要:

为明确五味子果肉和种子精油成分和生物活性的异同,通过气质联用仪对五味子果肉精油(PEO)和种子精油(SEO)进行成分分析,还对2种精油的抗肿瘤活性、抑菌活性和体外抗氧化活性等生物活性进行了对比研究。分析结果显示:从PEO和SEO中分别鉴定出55种和45种成分,质量分数分别占精油总量94.04%和93.39%。抗肿瘤活性显示:PEO和SEO对HeLa和BGC-823肿瘤细胞均具有较好的抗肿瘤活性(IC50值为39.07~56.43 mg/L),但2种精油对A549细胞均不显示抗肿瘤活性。抑菌活性检测结果表明:PEO和SEO对金黄色葡萄球菌、枯草芽孢杆菌、绿脓杆菌和大肠杆菌等4种细菌生长均具有一定抑制作用,PEO的MIC值为245.00~504.33 mg/L,SEO的MIC值为495.00~1 004.68 mg/L。此外,实验结果还显示PEO较SEO有更好的体外抗氧化活性,其中PEO对DPPH·和ABTS+·的IC50值分别为3.15和2.31 g/L,SEO对DPPH·和ABTS+·的IC50值分别为5.43和10.11 g/L。

关键词: 五味子, 精油, 抗肿瘤活性, 抑菌活性, 抗氧化活性

Abstract:

In order to investigate the differences and similarities between the essential oils (EOs) obtained from Schisandra chinensis fruits pulp and seeds (PEO and SEO), the chemical composition, anti-tumor property, antimicrobial and antioxidant activity were examined. According to the results of GC-MS (gas chromatography-mass spectrometry, GC-MS) analyses, 55 and 45 compounds which represented 94.04% and 93.39% of the oils were identified, respectively. In addition, anti-tumor activity of essential oils was carried out using the Sulforhodamine B (SRB) method. The oils also revealed the highest anti-tumor activity against HeLa and BGC-823 cells, but they had no effect on A549 cells. They presented IC50 values from 39.07 mg/L to 56.43 mg/L. PEO and SEO also exhibited inhibitory activity against four bacterial strains including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. MIC of PEO were 245.00-504.33 mg/L, MIC of SEO were 495.00-1 004.68 mg/L. Furthermore, in all assays of antioxidant ability, PEO showed stronger antioxidant properties than SEO. IC50 value of PEO against DPPH and ABTS radical were 3.15 and 2.31 g/L, respectively. IC50 of SEO against DPPH and ABTS radical were 5.43 and 10.11 g/L, respectively. In a word, this study supports the potentials of PEO and SEO as antibacterial and antineoplastic agent.

Key words: Schisandra chinensis, essential oils, anti-tumor activity, antibacterial activity, antioxidant activity

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