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林产化学与工业 ›› 2019, Vol. 39 ›› Issue (6): 115-122.doi: 10.3969/j.issn.0253-2417.2019.06.016

• 研究报告 • 上一篇    下一篇

红豆杉枝叶中总生物碱的提取及其抗肿瘤活性研究

姜萍(),赵雅洁,包信业,王飞,段占泉,王茂霖   

  1. 南京林业大学 化学工程学院, 江苏 南京 210037
  • 收稿日期:2019-08-06 出版日期:2019-12-28 发布日期:2019-12-21
  • 作者简介:姜萍(1969—   ),女,黑龙江伊春人,副教授,硕士生导师,主要从事天然产物化学与应用研究; E-mail:pingj_chem@njfu.edu.cn
  • 基金资助:
    国家重点研发计划资助项目(2017YFD0601306);江苏高校品牌专业建设工程项目(PPZY2015C221“林产化工”)

Extraction Technology of Total Alkaloids from Taxus chinensis Branches and Leaves and Their Antitumor Activities

Ping JIANG(),Yajie ZHAO,Xinye BAO,Fei WANG,Zhanquan DUAN,Maolin WANG   

  1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2019-08-06 Online:2019-12-28 Published:2019-12-21
  • Supported by:
    国家重点研发计划资助项目(2017YFD0601306);江苏高校品牌专业建设工程项目(PPZY2015C221“林产化工”)

摘要:

以红豆杉枝叶为原料,通过单因素试验和响应面法优化了红豆杉枝叶中总生物碱的酶与超声波辅助耦合提取工艺,并对粗总生物碱进行了体外抗肿瘤活性实验。研究结果表明:酶与超声波辅助耦合提取总生物碱的最佳工艺条件为原料量3.00 g,pH值为3的19%乙醇溶液作溶剂、酶质量浓度0.10 g/L、液料比12:1(mL:g)、酶解时间52 min、酶解温度50℃和超声波功率150 W,在此条件下提取1次的总生物碱得率为0.126%。响应面模型显著性检验发现模型的P < 0.000 1,表明差异极显著,回归方程有效,模型具有统计学意义。粗总生物碱萃取物的体外抗肿瘤活性结果表明:红豆杉枝叶粗总生物碱萃取物对3种肿瘤细胞都有较好的抑制作用,特别是对人宫颈癌(Hela)细胞的抑制活性最强,其半数抑制浓度(IC50)值为22.18 mg/L,抑制效果与阳性对照依托泊苷相当(IC50值为21.2 mg/L);其次对人乳腺癌(MCF7)细胞的抑制活性较强,其IC50值为74.95 mg/L;而对人肝癌(HepG2)细胞有一定的抑制活性,其IC50值为146.03 mg/L。

关键词: 红豆杉枝叶, 总生物碱, 提取工艺, 抗肿瘤活性

Abstract:

The optimum extraction conditions of total alkaloids from Taxus chinensis branches and leaves(TCBL)through enzymolysis treatment and ultrasonic-assisted extraction method were investigated by the single factor experiment and response surface experiment. Then, in vitro antitumor activities of crude total alkaloids extract were studied. The results showed that the optimum process conditions of enzyme and ultrasonic-assisted extraction are ethanol volume fraction of 19%, enzyme solution pH value 3.0, enzyme concentration of 0.10 g/L, liquid-solid ratio 12:1(mL:g), enzymolysis time 52 min, enzymolysis temperature 50℃, ultrasonic power 150 W.The yield of total alkaloid of extracting 1 time was 0.126% under these conditions. Response surface model significance test found that the model P < 0.000 1, which indicated that the difference was very significant, regression equation was effective, and the model had statistical significance.The in vitro antitumor activity of crude total alkaloids extracted from TCBL was studied. The results showed that total alkaloids extract from TCBL had good inhibitory effects on three kinds of cancer cells. Especially, the antitumor activity against human cervical cancer (Hela) cells was the strongest, and its IC50 value was 22.18 mg/L that was similar to that of etoposide (21.2 mg/L). It had better antitumor effect on human breast cancer (MCF7) cells (IC50 74.95 mg/L). It had a certain inhibitory activity on human hepatocellular carcinoma (HepG2) cells, and its IC50 value was 146.03 mg/L.

Key words: Taxus chinensis branches and leaves, total alkaloids, extraction technology, antitumor activity

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