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林产化学与工业 ›› 2020, Vol. 40 ›› Issue (6): 30-36.doi: 10.3969/j.issn.0253-2417.2020.06.005

• 重点研发专栏 • 上一篇    下一篇

蓝靛果籽油提取工艺优化及其脂肪酸组成分析

蒋一苇, 郭娜, 刘志国, 赵春建, 付玉杰, 王希清()   

  1. 东北林业大学 森林植物生态学教育部重点实验室; 林业生物制剂教育部工程研究中心; 化学化工与资源利用学院, 黑龙江 哈尔滨 150040
  • 收稿日期:2020-09-22 出版日期:2020-12-28 发布日期:2020-12-29
  • 通讯作者: 王希清 E-mail:wangxiqing0622@163.com
  • 作者简介:王希清, 博士, 研究领域为生物质资源利用; E-mail:wangxiqing0622@163.com
    蒋一苇(1996-), 女, 黑龙江鹤岗人, 硕士生, 主要从事生药学研究工作
  • 基金资助:
    国家重点研发计划资助项目(2016YFD0600805);高等学校学科创新引智计划(B20088);黑龙江头雁创新团队项目(无编号)

Optimization of Extraction Technology of Blue Honeysuckle Seed Oil and Analysis of Its Fatty Acid Composition

Yiwei JIANG, Na GUO, Zhiguo LIU, Chunjian ZHAO, Yujie FU, Xiqing WANG()   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education; Engineering Research Center of Forest Bio-preparation, Ministry of Education; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
  • Received:2020-09-22 Online:2020-12-28 Published:2020-12-29
  • Contact: Xiqing WANG E-mail:wangxiqing0622@163.com

摘要:

采用微波辅助无水乙醇提取蓝靛果籽油,对提取时间、料液比、提取温度和微波功率进行了单因素试验,在此基础上采用响应面法进一步优化了提取工艺,并对蓝靛果籽油脂肪酸成分进行了分析。研究结果表明:10 g蓝靛果籽油的最佳提取工艺为提取时间23 min、液料比23:1(mL:g),提取温度50℃和微波功率500 W,此条件下的蓝靛果籽油得率为(26.0±0.05)%。利用气相色谱-质谱联用分析技术在蓝靛果籽油中主要检测到7种脂肪酸(与索氏提取法所得蓝靛果籽油的脂肪酸组成相同),其中主要成分是2种共轭亚油酸,分别为9,顺-11,反-十八碳二烯酸(23.49%)和10,反-12,顺-十八碳二烯酸(21.33%)。

关键词: 蓝靛果籽油, 微波辅助提取, 气相色谱-质谱联用, 响应面

Abstract:

The microwave-assisted extraction method was selected to extract blue honeysuckle(Lonicera caerulea var.enulis Turcz et Herd) seed oil and the fatty acid composition of the seed oil was analyzed. Single factor experiments were carried out on extraction time, liquid-solid ratio, extraction temperature, and microwave power. Then, the process for microwave-assisted alcohol extraction of L.caerulea seed oil was optimized by response surface methodology based on the single factor experiments. The results indicated that the optimal extraction conditions were as follows: extraction time 23 min, liquid-to-solid ratio 23:1(mL:g), extraction temperature 50 ℃ and microwave power 500 W, and the yield of L.caerulea seed oil was (26.0±0.05)% under these conditions. The composition of the L.caerulea seed oil was analyzed by GC-MS and 7 fatty acids were identified as the main components, most of which were two conjugated linoleic acids, cis-9, trans-11-octadecadienoate and trans-10, cis-12-octadecadienoate and the relative contents were 23.49% and 21.33%, respectively.

Key words: blue honeysuckle seed oil, microwave-assisted extraction, GC-MS, response surface methodology

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