圆齿野鸦椿果皮多糖的提取及其抗炎活性研究

doi:10.3969/j.issn.0253-2417.2021.03.011

摘要/Abstract

摘要：

圆齿野鸦椿是一种极具利用价值的植物，为了开发利用其果皮多糖资源，对圆齿野鸦椿果皮多糖提取工艺和抗炎活性进行研究。以单因素试验和响应面法对提取条件进行优化，结果表明：当液料比30：1（mL：g）、超声波功率400 W、提取时间100 min、提取温度60℃时圆齿野鸦椿果皮多糖的提取得率可高达29.15%。用DEAE-52纤维柱对粗多糖分离提纯后，得到一个主要的圆齿野鸦椿果皮多糖组分（PEP）。PEP经柱前衍生化法检测，发现其中含有10种单糖，分别为甘露糖5.65%、葡萄糖37.06%、半乳糖19.51%、岩藻糖2.24%、木糖26.10%、核糖1.33%、葡萄糖醛酸0.89%、鼠李糖2.33%、半乳糖醛酸0.68%和阿拉伯糖4.20%。且1~10 g/L的PEP能有效抑制RAW264.7巨噬细胞炎症因子的分泌，但对细胞存活率无显著影响。

关键词: 多糖, 提取, 单糖组成, 抗炎活性

Abstract:

To develop and utilize the polysaccharide resources from Euscaphis konishii Hayata, the extraction technology and anti-inflammatory effect of polysaccharides from E. konishii pericarp were studied. The extraction process was optimized by single-factor experiments and response surface methodology. Results showed that under the conditions of liquid to material ratio of 30:1(mL: g), ultrasonic power of 400 W, extraction at 60℃ for 100 min, the polysaccharide yield could reach up to 29.15%. The crude polysaccharides were separated and purified by DEAE-52 cellulose column chromatography to get a main E. konishii pericarp polysaccharide(PEP). The monosaccharide compositions of PEP were analyzed by HPLC with pre-column derivatization. The results showed that PEP contained ten monosaccharides including mannose 5.65%, glucose 37.06%, galactose 19.51%, fucose 2.24%, xylose 26.10%, ribose 1.33%, gluconose 0.89%, rhamnose 2.33%, galactose aldose 0.68% and arabinose 4.20%. PEP with the mass concentrations of 1-10 g/L inhibited the LPS-induced secretion of inflammatory factors in RAW264.7 macrophages, but had no significant effect on the cell viability, which suggested that PEP possessed anti-inflammatory capacity, and had a good prospect for development and utilization.

Key words: polysaccharides, extraction, monosaccharides composition, anti-inflammatory capacity

中图分类号:

黄维, 郭梦云, 王玉启, 林金垒, 张宇翔, 邹双全. 圆齿野鸦椿果皮多糖的提取及其抗炎活性研究[J]. 林产化学与工业, 2021, 41(3): 77-84.

Wei HUANG, Mengyun GUO, Yuqi WANG, Jinlei LIN, Yuxiang ZHANG, Shuangquan ZOU. Extraction and Anti-inflammatory Effect of Polysaccharides from Euscaphis konishii Hayata Pericarp[J]. Chemistry and Industry of Forest Products, 2021, 41(3): 77-84.

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来源 source	平方和 quadratic sum	自由度 freedom degree	均方 mean square	F值 F value	P值 P value
模型	367.59	14	26.26	210.95	< 0.0001^**
X₁	317.50	1	317.50	2550.85	< 0.0001^**
X₂	0.63	1	0.63	5.04	0.0414^*
X₃	0.11	1	0.11	0.89	0.3606
X₄	0.032	1	0.032	0.25	0.6226
X₁X₂	2.12	1	2.12	17.01	0.001^**
X₁X₃	0.80	1	0.80	6.40	0.024^*
X₁X₄	0.13	1	0.13	1.04	0.001^**
X₂X₃	1.28	1	1.28	10.30	0.3346
X₂X₄	1.48	1	1.48	11.86	0.057
X₃X₄	4.70	1	4.70	37.75	0.04^*
X₁²	14.87	1	14.87	119.46	< 0.0001^**
X₂²	15.77	1	15.77	126.66	< 0.0001^**
X₃²	7.14	1	7.14	57.35	< 0.0001^**
X₄²	21.26	1	21.26	170.78	< 0.0001^**
残差residual error	1.74	14	0.12
失拟项lack offit	1.45	10	0.15	2.02	0.2574
纯误差pure error	0.29	4	0.072
总值total value	369.33	28

实验组 test group	药物质量浓度/(g·L^-1) drug mass concentration	NO/(μmol·L^-1)	PGE2/(ng·L^-1)	细胞存活率/% cell viability
给药组treatment group	1	63.71±5.29^*	414.26±17.53^**	104.49±8.32
	2	45.30±4.72^**	366.65±16.89^**	96.46±6.69
	5	39.05±4.48^**	332.98±12.47^**	107.80±7.93
	10	35.43±4.61^**	319.43±13.92^**	99.17±8.16
空白对照组control group	—	8.83±0.46	180.32±9.44	100.00±7.56
模型组model group	—	80.69±6.27	492.57±20.52	95.37±6.54
阳性对照组positive control group	0.2	22.45±3.06^##	267.80±12.78^##	94.78±7.06

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