杨木联产低聚木糖和枯草芽孢杆菌的研究

doi:10.3969/j.issn.0253-2417.2021.05.001

摘要/Abstract

摘要：

采用乙酸(AC)水解杨木制备低聚木糖(XOS)，然后探讨不同第二步预处理对乙酸水解杨木酶水解的影响，最后以酶水解液作为碳源进行发酵培养枯草芽孢杆菌。采用5%乙酸在170℃下预处理杨木30 min后，低聚木糖得率为55.8%。第二步预处理中，各种预处理对乙酸杨木酶水解改善效果的顺序依次是：乙酸-双氧水(HPAC)、NaOH、NaOH-H₂O₂、γ-戊内酯(GVL)、NH₃·H₂O预处理。乙酸水解杨木再经过乙酸-双氧水预处理后的纤维素酶的水解液中葡萄糖得率最高为72.4%。加入吐温80可以使乙酸和乙酸-双氧水(AC-HPAC)两步预处理的杨木酶水解液中的葡萄糖得率提升至93.8%。两步预处理得到的酶水解液经过11.5 h发酵后，培养的枯草芽孢杆菌按菌落形成单位(CFU)计数，菌体浓度最高可达2.1×10⁹ CFU/mL，此时酶水解中的葡萄糖利用率为95.3%，木糖利用率为25.0%。

关键词: 杨木, 预处理, 低聚木糖, 酶水解, 枯草芽孢杆菌

Abstract:

Acetic acid(AC) hydrolysis and the second-step pretreatment were used to produce xylooligosaccharides(XOS) and enzymatic hydrolysate from poplar. The hydrolysate was used as carbon resource to produce Bacillus subtilis by fermentation. After the poplar was pretreated by 5% AC at 170℃ for 30 min, the XOS yield of the pretreatment liquor was 55.8%. In the second-step pretreatment, the order of improvement on enzymatic hydrolysis of AC-pretreated poplar was showed as follows: hydrogen peroxide-acetic acid(HPAC), sodium hydroxide, sodium hydroxide-hydrogen peroxide, γ-valerolactone(GVL), ammonia pretreatment. The AC-pretreated poplar after HPAC pretreatment had the highest digestiability and the glucose yield of the hydrolysate was 72.4%. Specially, Tween 80 could further improve the glucose yield of AC-HPAC-pretreated poplar to 93.8%. The enzymatic hydrolysate obtained from the two-step pretreated poplar was used to produce B. subtilis and the highest viable count of the fermentation liquor could reach 2.1×10⁹ CFU/mL. After fermentation for 11.5 h, the utilizations of glucose and xylose were 95.3% and 25.0%, respectively. The results in this work could guide for the co-production of XOS and B. subtilis from poplar, and provide a reference for the high value-added conversion of poplar.

Key words: poplar, pretreatment, xylooligosaccharides, enzymatic hydrolysis, Bacillus subtilis

中图分类号:

TQ35

文沛瑶, 游佳欣, 徐勇, 张军华. 杨木联产低聚木糖和枯草芽孢杆菌的研究[J]. 林产化学与工业, 2021, 41(5): 1-7.

Peiyao WEN, Jiaxin YOU, Yong XU, Junhua ZHANG. Co-production of Xylooligosaccharides and Bacillus subtilis from Poplar[J]. Chemistry and Industry of Forest Products, 2021, 41(5): 1-7.

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参考文献 19

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预处理¹⁾ pretreatment	温度/℃ temperature	时间/h time	纤维素/% cellulose	木聚糖/% xylan	酸不溶木质素/% acid insoluble lignin	酸溶木质素/% acid soluble lignin
NaOH	120	1	74.1±0.2	2.4±0.1	22.1±1.6	0.9±0.2
NaOH-H₂O₂	120	1	70.8±0.2	1.3±0.1	25.7±0.6	0.8±0.6
NH₃·H₂O	70	24	64.5±0.2	5.9±0.1	27.8±0.4	1.9±0.4
GVL	140	1	74.1±0.4	5.4±0.2	16.5±0.1	0.7±0.2
HPAC	60	2	87.8±0.2	9.2±0.4	4.3±0.1	0.1±0.0

预处理 pretreatment	温度/℃ temperature	时间/h time	纤维素/% cellulose	木聚糖/% xylan	酸不溶木质素/% acid insoluble lignin	酸溶木质素/% acid soluble lignin
NaOH	120	1	75.8±0.2	1.4±0.1	23.2±0.4	0.9±0.3
NaOH-H₂O₂	120	1	75.1±0.2	3.5±0.1	22.0±0.4	0.6±0.1
NH₃·H₂O	70	24	66.4±0.2	3.7±0.1	28.9±0.4	1.5±0.2
GVL	140	1	86.7±0.2	2.7±0.1	10.3±0.4	0.3±0.5
HPAC	60	2	90.4±0.0	3.0±0.3	0.3±0.0	0.1±0.0

预处理 pretreatment	温度/℃ temperature	时间/h time	葡萄糖得率/% glucose yield	木糖得率/% xylose yield
NaOH	120	1	35.2±0.1	32.4±5.2
NaOH-H₂O₂	120	1	28.9±0.1	58.6±3.1
NH₃·H₂O	70	24	18.6±0.5	16.7±2.1
GVL	140	1	25.7±2.0	20.9±2.0
HPAC	60	2	67.2±0.5	72.4±0.9

预处理 pretreatment	温度/℃ temperature	时间/h time	葡萄糖得率/% glucose yield	木糖得率/% xylose yield
NaOH	120	1	36.2±0.3	63.1±1.1
NaOH-H₂O₂	120	1	31.3±2.4	23.6±4.2
NH₃·H₂O	70	24	23.9±0.7	21.2±0.7
GVL	140	1	25.0±0.2	26.8±0.8
HPAC	80	2	62.3±0.5	71.0±3.7

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