欢迎访问《林产化学与工业》,

林产化学与工业 ›› 2021, Vol. 41 ›› Issue (5): 85-92.doi: 10.3969/j.issn.0253-2417.2021.05.013

• 研究报告 • 上一篇    下一篇

木瓜蛋白酶辅助提取丹皮中多酚、总黄酮的响应面优化及抗氧化活性研究

马晓1(), 陈刚2   

  1. 1. 河南职业技术学院, 河南 郑州 450046
    2. 郑州师范学院, 河南 郑州 450044
  • 收稿日期:2020-10-19 出版日期:2021-10-28 发布日期:2021-11-04
  • 作者简介:马晓(1980-), 女, 河南漯河人, 副教授, 硕士, 主要从事生物活性物质提取及功能研究; E-mail: maxiao720@163.com
  • 基金资助:
    河南省科技攻关项目(172102110219)

Response Surface Optimization of Papain-assisted Extraction of Polyphenol and Flavonoids form Paeonia suffruticosa Cortex and Its Antioxidant Activity

Xiao MA1(), Gang CHEN2   

  1. 1. Henan Polytechnic College, Zhengzhou 450046, China
    2. Zhengzhou Normal University, Zhengzhou 450044, China
  • Received:2020-10-19 Online:2021-10-28 Published:2021-11-04

摘要:

为了解牡丹干燥根皮(丹皮)多酚、总黄酮的体外抗氧化活性,以木瓜蛋白酶酶解丹皮粉末,提取丹皮多酚和总黄酮,选取多酚得率、总黄酮得率和DPPH自由基(DPPH·)清除率为考察指标,采用响应面法对提取条件进行优化。通过建立模型并分析得到3个指标分别达到最优时的因素水平,以及3个指标同时达到较优时的因素水平并进行验证。研究结果表明:多酚得率的最佳因素水平为酶添加量400 U/g,液料比21:1(mL:g,下同),pH值5.5,酶解119 min,该条件下多酚的得率为28.96 mg/g;总黄酮得率的最佳因素水平为酶添加量400 U/g,液料比20:1,pH值6,酶解121 min,该条件下总黄酮得率为44.32 mg/g;DPPH·清除率的最佳因素水平组合为酶添加量400 U/g,液料比21:1,pH值6,酶解123 min,该条件下DPPH·清除率为66.87%。3个指标同时达到较优的因素水平为酶添加量400 U/g,液料比21:1,pH值6,酶解122 min,该条件下多酚得率为28.25 mg/g,总黄酮得率为42.96 mg/g,DPPH·清除率为66.72%,此优化条件可靠,得率较为理想,可为木瓜蛋白酶酶法提取多酚和总黄酮提供一定的指导。

关键词: 丹皮, 木瓜蛋白酶, 多酚, 总黄酮, DPPH自由基清除率

Abstract:

To explore the extraction process and in vitro antioxidant activity of polyphenol and flavonoids, papain was used to enzymatically hydrolyze moutan cortex powder to extract paeonol and flavonoids. The yield of polyphenol, flavonoids and the scavenging rate of DPPH free radicals(DPPH·) were selected as the indexes to optimize the extraction conditions by response surface methodology. Though analysing the established model which verified reliably, the optimal extrating conditions were obtained when the three indexes reached the maximum level separately and simultaneously. The results showed that the optimal extraction process parameters of polyphenol yield were papain 400 U/g, liquid to material ratio 21:1(mL: g), pH 5.5, and enzymatic hydrolysis time 119 min with the yield of polyphenol was 28.96 mg/g. The optimal extraction process parameters of flavonoid yield were papain 400 U/g, liquid to material ratio 20:1(mL: g), pH 6.0, enzymatic hydrolysis time 121 min, with theyield of flavonoids was 44.32 mg/g.The optimal extraction process parameters of DPPH· scavenging rate were papain 400 U/g, liquid to material ratio 21:1(mL: g), pH 6, and enzymatic hydrolysis time 123 min with DPPH· scavenging rate was 66.87%. The optimal extraction process parameter of the yield of polyphenol, flavonoid yield and DPPH· scavenging rate was papain 400 U/g, the ratio of liquid to material 21:1(mL: g), pH 6, and the enzymatic hydrolysis time 122 min with the yield of polyphenol was 28.25 mg/g, the yield of flavonoid was 42.96 mg/g and the DPPH· scavenging rate was 66.72%. The obtained model was reliable, and the prediction of the yield of polyphenol and flavonoids and the scavenging rate of DPPH· was accurate.

Key words: moutan cortex, papain, polyphenol, flavonoids, DPPH free radicals scavenging rate

中图分类号: