云南金花茶叶不同极性提取物的抗氧化活性分析

doi:10.3969/j.issn.0253-2417.2022.05.009

摘要/Abstract

摘要：

以水、石油醚、氯仿、乙酸乙酯和正丁醇等不同极性溶剂对云南金花茶叶片中的多酚和总黄酮进行提取，以DPPH·、·ABTS⁺和·OH清除率以及总还原能力为考察指标，分析云南金花茶不同极性溶剂提取物的抗氧化能力。研究结果表明：不同极性溶剂对云南金花茶中多酚和总黄酮的提取能力不同，且提取物抗氧化能力存在差异。对多酚的提取能力依次为：水＞乙酸乙酯＞石油醚＞氯仿＞正丁醇，对总黄酮的提取能力依次为：水＞乙酸乙酯＞氯仿＞石油醚＞正丁醇。云南金花茶有较好的抗氧化活性，不同极性溶剂提取物的抗氧化能力之间存在一定差异，水相和乙酸乙酯相的总体抗氧化能力高于氯仿相、正丁醇相和石油醚相。水相提取物对DPPH·和·ABTS⁺的清除率最强，其半数抑制质量浓度(IC₅₀)值分别为(38.634±2.556)和(276.610±8.416)mg/L，乙酸乙酯相((50.845±3.985)和(362.754±22.555)mg/L)次之；石油醚相对·OH清除率最强，其IC₅₀值为(180.880±49.568)mg/L、氯仿相((1 130.630±157.932)mg/L)次之。当云南金花茶不同极性提取物质量浓度为1 750 mg/L时，水相的总还原力最强，其吸光值为2.281±0.103、乙酸乙酯相(1.504±0.021)次之。

关键词: 云南金花茶, 不同极性提取物, 多酚, 总黄酮, 抗氧化活性

Abstract:

In this study, polyphenols and total flavonoids were extracted from Camellia fascicularis H. T. Chang using different polar solvents such as water, petroleum ether, chloroform, ethyl acetate, and n-butanol. The radical scavenging rate on DPPH·, ·ABTS⁺, and ·OH and the reducing power were used as indicators to analyze the antioxidant capacity of different polar solvents extracts from C. fascicularis. The results showed that extraction abilities for polyphenols and total flavonoids from C. fascicularis by different polar extract solvents and the antioxidant capacities of different polar extracts were different. The order of extraction abilities for polyphenols and total flavonoids were as follows: water>ethyl acetate>petroleum ether>chloroform>n-butanol and water>ethyl acetate>chloroform>petroleum ether>n-butanol, respectively. C. fascicularis showed good antioxidant activities, and there were some differences in antioxidant capacities of different polar extracts. The comprehensive antioxidant capacities of water and ethyl acetate phases were higher than that of chloroform, n-butanol, and petroleum ether phases. The water phase extract exhibited the strongest scavenging capacities for DPPH· and ·ABTS⁺, and the IC₅₀ values were (38.634±2.556) and (276.610±8.416)mg/L, respectively, followed by the ethyl acetate phase((50.845±3.985) and (362.754±22.555) mg/L). The petroleum ether phase showed the strongest scavenging capacities for ·OH with an IC₅₀ value of (180.88±49.568) mg/L, followed by the chloroform phase((1 130.630±157.932)mg/L). The water phase demonstrated the strongest total reducing power with an absorbance value of 2.281±0.103 at the mass concentration of different polar extracts of 1 750 mg/L, followed by the ethyl acetate phase(1.504±0.021).

Key words: Camellia fascicularis H. T. Chang, different polar extracts, polyphenols, total flavonoids, antioxidant capacity

中图分类号:

TQ35

何旭华, 石志娇, 彭小伟, 阚欢, 赵平, 刘云. 云南金花茶叶不同极性提取物的抗氧化活性分析[J]. 林产化学与工业, 2022, 42(5): 61-68.

Xuhua HE, Zhijiao SHI, Xiaowei PENG, Huan KAN, Ping ZHAO, Yun LIU. Antioxidant Activities Analysis of Different Polar Solvent Extracts from Camellia fascicularis H. T. Chang[J]. Chemistry and Industry of Forest Products, 2022, 42(5): 61-68.

图/表 5

表1

图1

表2

云南金花茶不同极性提取物对自由基清除率和总还原力的曲线方程及IC50值"

样品 sample	自由基 free radicals	拟合方程 fitting equation	R²	IC₅₀/(mg·L^-1)
Vc	DPPH·	Y=-0.003 24X²+0.123 15X-0.060 47	0.964	5.287±0.309
	·ABTS⁺	Y=(-2.00×10^-5)X²+0.009 20X-0.013 01	0.978	64.928±0.725
	·OH	Y=-0.082 57X²+0.457 73X-0.372 86	0.995	293.290±36.077
	总还原能力 reducing power	Y=(-2.26×10^-6)X²+0.006 28X-0.174 21	0.986
水相 water phase	DPPH·	Y=(-6.08×10^-5)X²+0.015 15X+0.005 54	0.989	38.634±2.556
	·ABTS⁺	Y=(-4.89×10^-7)X²+0.001 31X+0.175 35	0.966	276.610±8.416
	·OH	Y=-0.006 17X²+0.114 40X+0.244 12	0.990	2 601.500±164.756
	总还原能力 reducing power	Y=(-3.20×10^-7)X²+0.001 83X+0.033 13	0.992
乙酸乙酯相 ethyl acetate phase	DPPH·	Y=(-2.55×10^-5)X²+0.009 40X+0.087 72	0.992	50.845±3.985
	·ABTS⁺	Y=(-4.15×10^-7)X²+0.001 23X+0.108 67	0.998	362.754±22.555
	·OH	Y=-0.005 9X²+0.087 91X+0.318 74	0.954	2 472.160±826.007
	总还原能力 reducing power	Y=(-1.70×10^-7)X²+0.001 15X+0.019 53	0.999
氯仿相 chloroform phase	DPPH·	Y=(-3.98×10^-6)X²+0.004 77X+0.212 75	0.984	63.598±3.854
	·ABTS⁺	Y=(-1.45×10^-7)X²+0.000 67X+0.131 69	0.998	643.692±29.651
	·OH	Y=-0.020 21X²+0.248 14X+0.245 28	0.959	1 130.630±157.932
	总还原能力 reducing power	Y=(-2.39×10^-8)X²+(5.94×10^-4)X+0.092 12	0.995
正丁醇相 n-butanol phase	DPPH·	Y=(-1.30×10^-5)X²+0.006 00X-0.032 66	0.993	119.810±8.207
	·ABTS⁺	Y=(-1.22×10^-7)X²+0.000 64X+0.038 72	0.996	865.188±35.706
	·OH	Y=-0.007 82X²+0.140 23X+0.069 13	0.999	3 936.230±621.700
	总还原能力 reducing power	Y=(6.92×10^-10)X²+(4.18×10^-4)X+0.097 8	0.998
石油醚相 petroleum ether phase	DPPH·	Y=(-1.04×10^-5)X²+0.008 41X-0.011 07	0.998	66.197±5.950
	·ABTS⁺	Y=(-3.65×10^-8)X²+0.000 40X+0.054 09	0.998	1 276.462±39.405
	·OH	Y=-0.076 52X²+0.442 93X+0.422 39	0.993	180.880±49.568
	总还原能力 reducing power	Y=(2.07×10^-8)X²+(2.64×10^-4)X+0.097 57	0.993

表2

图2

表3

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样品 sample	多酚/(mg·g^-1) polyphenols	总黄酮/(mg·g^-1) total flavonoids
石油醚相 petroleum ether phase	90.181±3.384^c	50.500±2.508^d
氯仿相 chloroform phase	84.414±2.298^c	75.148±3.792^c
乙酸乙酯相 ethyl acetate phase	124.578±2.132^b	92.844±6.021^b
正丁醇相 n-butanol phase	62.866±2.278^d	26.484±3.047^e
水相 water phase	140.259±5.989^a	102.324±1.095^a

项目 item	IC₅₀(DPPH·)	IC₅₀(·ABTS⁺)	IC₅₀(·OH)	总还原能力 total reducing power	多酚 polyphenols	总黄酮 total flavonoids
IC₅₀(DPPH·)	1	0.779^**	0.224	-0.786^**	-0.889^**	-0.914^**
IC₅₀(·ABTS⁺)	0.779^**	1	-0.161	-0.971^**	-0.704^**	-0.794^**
IC₅₀(·OH)	0.224	-0.161	1	0.133	-0.121	-0.201
总还原能力 reducing power	-0.786^**	-0.971^**	0.133	1	0.864^**	0.832^**

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