基于UV-Vis光谱法的靛蓝提取物光氧化降解研究

doi:10.3969/j.issn.0253-2417.2022.06.005

林产化学与工业 ›› 2022, Vol. 42 ›› Issue (6): 31-38.doi: 10.3969/j.issn.0253-2417.2022.06.005

基于UV-Vis光谱法的靛蓝提取物光氧化降解研究

刘丹阳(), 颜洋洋, 王成章()

中国林业科学研究院林产化学工业研究所；江苏省生物质能源与材料重点实验室；国家林业和草原局林产化学工程重点实验室；林木生物质低碳高效利用国家工程研究中心；江苏省林业资源高效加工利用协同创新中心，江苏南京 210042

收稿日期:2021-11-03 出版日期:2022-12-28 发布日期:2023-01-07
通讯作者: 王成章 E-mail:1009828079@qq.com;wangczlhs@sina.com
作者简介:王成章，研究员，博士，博士生导师，主要从事天然产物研究与利用；E-mail: wangczlhs@sina.com
刘丹阳(1995—)，女，河南濮阳人，研究实习员，硕士，主要从事天然产物的研究与开发；E-mail: 1009828079@qq.com
基金资助:
中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2021ZC003)

Photooxidative Degradation of Indigo Extract by UV-Vis Spectroscopy

Danyang LIU(), Yangyang YAN, Chengzhang WANG()

Institute of Chemical Industry of Forest Products, CAF; Key Lab. of Biomass Energy and Material, Jiangsu Province; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing 210042, China

Received:2021-11-03 Online:2022-12-28 Published:2023-01-07
Contact: Chengzhang WANG E-mail:1009828079@qq.com;wangczlhs@sina.com

摘要/Abstract

摘要：

以合成靛蓝标准品的光氧化降解为模型，通过UV-Vis光谱法研究靛蓝在二甲基亚砜溶剂中的降解规律，并考察了质量浓度、光照时间及光照方式对靛蓝降解的影响，进一步研究了靛蓝提取物(IE)的光氧化降解变化过程及产物。研究结果表明：在自然光条件下，浓度越高的靛蓝溶液降解速率越快，随着时间延长，靛蓝溶液的降解速率先快后慢，前5天降解最明显，17天后超过90%的靛蓝已经降解，直至第24天不再降解，最大吸收波长(618 nm)处吸光度值随时间整体呈指数变化。能量较高的紫外光对靛蓝溶液降解的影响强于太阳光和手电筒白光。HPLC图谱分析发现：靛蓝标准品和靛蓝提取物降解生成靛红的比例分别为56.78%和85.50%，证明合成和天然靛蓝降解主要产物均为靛红。

关键词: 靛蓝, 降解, 光照, 靛红, UV-Vis光谱法

Abstract:

Taking the photodegradation of synthetic indigo as a model, the degradation of indigo in dimethyl sulfoxide solvent was studied by UV-Vis spectroscopy, and the effects of mass concentration, illumination time and illumination methods on the degradation of indigo were investigated. The process and products of photodegradation of indigo extract(IE) were further studied. The results showed that the higher the concentration of indigo solution, the faster the degradation rate under natural light conditions was achieved. With the extension of time, the degradation rate of indigo solution was first fast and then slow. The degradation was most obvious in the first 5 days. After 17 days, more than 90% of indigo had been degraded, and the degradation was stopped until the 24^th day. The absorbance value at the maximum absorption changed exponentially with time. The effect of ultraviolet light with higher energy on the degradation of indigo solution was stronger than that of sunlight and white flashlight. HPLC analysis showed that the proportion of isatin produced by the degradation of synthetic indigo standard and indigo extract were 56.78% and 85.50%, respectively, which proved that the main product of the degradation of synthetic and natural indigo was isatin.

Key words: indigo, degradation, illumination, isatin, UV-Vis spectroscopy

中图分类号:

TQ35

刘丹阳, 颜洋洋, 王成章. 基于UV-Vis光谱法的靛蓝提取物光氧化降解研究[J]. 林产化学与工业, 2022, 42(6): 31-38.

Danyang LIU, Yangyang YAN, Chengzhang WANG. Photooxidative Degradation of Indigo Extract by UV-Vis Spectroscopy[J]. Chemistry and Industry of Forest Products, 2022, 42(6): 31-38.

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基于UV-Vis光谱法的靛蓝提取物光氧化降解研究

Photooxidative Degradation of Indigo Extract by UV-Vis Spectroscopy

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