响应面优化皂角刺皂苷超声波辅助提取工艺及其降血糖活性

doi:10.3969/j.issn.0253-2417.2023.02.002

摘要/Abstract

摘要：

在单因素试验基础上, 采用响应面法优化超声波辅助提取皂角刺皂苷的最佳工艺, 并考察了最佳提取工艺下提取物对α-葡萄糖苷酶和α-淀粉酶的抑制活性。实验结果表明：皂角刺总皂苷超声波辅助提取的最佳工艺为液料比16∶1(mL∶g)、乙醇体积分数60%、超声波时间80 min和提取温度50 ℃, 在此条件下皂角刺总皂苷得率为13.28%±0.25%。最佳工艺下的提取物对α-葡萄糖苷酶的半数抑制质量浓度(IC₅₀)值为(0.146±0.019) g/L, 强于阳性对照阿卡波糖IC₅₀值((0.48±0.18) g/L)；当提取物质量浓度为2 g/L时, 对α-淀粉酶抑制率可达35.13%±0.58%, 表明皂角刺具有开发成为Ⅱ型糖尿病药物的潜力。

关键词: 皂角刺皂苷, 响应面, α-葡萄糖苷酶, α-淀粉酶

Abstract:

On the basis of single factor experiment, response surface methodology(RSM) was used to optimize the ultrasonic-assisted extraction optimum process of saponins from Gleditsia sinensis Thorn(GST), and the inhibitory activities of the extracts on α-glucosidase and α-amylase were investigated under the optimal extraction conditions. The results showed that the optimal ultrasonic-assisted extraction conditions were solid-liquid ratio of 16:1(mL: g), ethanol volume fraction of 60%, ultrasonic time of 80 min and extraction temperature of 50℃. Under these conditions, the yield of total saponins was 13.28%±0.25%, and the IC₅₀ value of α-glucosidase was (0.146±0.019) g/L, which was stronger than that of positive control acarbose with the median inhibitory concentration(IC₅₀) value of (0.48±0.18) g/L. When the mass concentration of the extract was 2 g/L, the inhibition rate of α-amylase could reach 35.13%±0.58%, indicating that GST had the potential development into a drug for type II diabetes.

Key words: saponins of Gleditsia sinensis Thorn, response surface methodology, α-glucosidase, α-amylase

中图分类号:

TQ35
R284

刘勇, 张彩虹, 谢普军, 邓叶俊, 黄立新. 响应面优化皂角刺皂苷超声波辅助提取工艺及其降血糖活性[J]. 林产化学与工业, 2023, 43(2): 12-18.

Yong LIU, Caihong ZHANG, Pujun XIE, Yejun DENG, Lixin HUANG. Optimization of Ultrasonic-assisted Extraction Technology of Saponins from Gleditsia sinensis Thorn by RSM and Its Hypoglycemic Activity[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 12-18.

图/表 5

图1

图2

表1

表2

图3

参考文献 26

1	李淑芳.HPLC法测定皂角刺中槲皮素和刺囊酸的含量[J].中医药临床杂志,2019,31(2):395-397.
	LI S F .Determination of quercetin and echinocystic acid in spina gleditsiae by HPLC[J].Clinical Journal of Traditional Chinese Medicine,2019,31(2):395-397.
2	SEO C S , LIM H S , HA H , et al.Quantitative analysis and anti-inflammatory effects of Gleditsia sinensis Thorns in RAW 264.7 macrophages and HaCaT keratinocytes[J].Molecular Medicine Reports,2015,3(12):4773-4781.
3	李岗, 王召平, 仙云霞, 等.皂角刺中黄酮类成分及其抗肿瘤活性研究[J].中草药,2016,47(16):2812-2816. doi: 10.7501/j.issn.0253-2670.2016.16.005
	LI G , WANG Z P , XIAN Y X , et al.Flavonoids from Gleditsiae spina and their antitumor activities[J].Chinese Traditional and Herbal Drugs,2016,47(16):2812-2816. doi: 10.7501/j.issn.0253-2670.2016.16.005
4	CHENG Y, HE W D, HE Y M. Gleditsia saponin C induces A549 cell apoptosis via caspase-dependent cascade and suppresses tumor growth on xenografts tumor animal model[J/OL]. Frontiers in Pharmacology, 2018, 8: 988[2021-12-20]. https://doi.org/10.3389/fphar.2017.00988.
5	楚红英, 罗晓, 李瑜.皂角刺多糖的提取及抗氧化活性的研究[J].理化检验(化学分册),2015,51(8):1063-1066.
	CHU H Y , LUO X , LI Y .Study of extraction and antioxidation of polysaccharides in Chinese Honeylocust spine[J].Physical Testing and Chemical Analysis(Part B: Chemical Analysis),2015,51(8):1063-1066.
6	LEE J , YI J M , KIM H , et al.Cytochalasin H, an active anti-angiogenic constituent of the ethanol extract of Gleditsia sinensis Thorns[J].Biological & Pharmaceutical Bulletin,2014,37(1):6-12.
7	RALLA T , SALMINEN H , TUOSTO J , et al.Formation and stability of emulsions stabilised by Yucca saponin extract[J].International Journal of Food Science & Technology,2018,53(6):1381-1388.
8	YU J Q , LI G , MU Y , et al.Anti-breast cancer triterpenoid saponins from the thorns of Gleditsia sinensis[J].Natural Product Research,2018,33(16):2308-2313.
9	TIAN X L , LI Y T , HAO N , et al.The antifeedant, insecticidal and insect growth inhibitory activities of triterpenoid saponins from Clematis aethusifolia Turcz against Plutella xylostella (L.)[J].Pest Management Science,2020,77(1):455-463.
10	SALMINEN H , BISCHOFF S , WEISS J .Formation and stability of emulsions stabilized by Quillaja saponin-egg lecithin mixtures[J].Journal of Food Science,2020,85(4):1213-1222.
11	REICHERT C L , SALMINEN H , WEISS J .Quillaja saponin characteristics and functional properties[J].Annual Review of Food Science and Technology,2019,10,43-73.
12	王洋, 庞康, 钟远坚, 等.超声辅助乙醇提取荔枝核总皂苷工艺优化及其抗炎作用研究[J].化学试剂,2020,42(10):1240-1245.
	WANG Y , PANG K , ZHONG Y J , et al.Ultrasonic assisted ethanol extraction method and anti-inflammatory activity of total saponins from Litchi seeds[J].Chemical Reagents,2020,42(10):1240-1245.
13	只德贤, 覃海波, 李建颖.微波超声协同提取金盏菊皂苷工艺及其抗氧化性研究[J].食品研究与开发,2021,42(19):118-125.
	ZHI D X , QIN H B , LI J Y .Microwave-ultrasonic extraction of Calendula officinalis saponins and their antioxidant analysis[J].Food Research and Development,2021,42(19):118-125.
14	ALATRACH M , AGYIN C , MEHTA R , et al.Glucose-mediated glucose disposal at baseline insulin is impaired in IFG[J].The Journal of Clinical Endocrinology and Metabolism,2019,104(1):163-171.
15	TAN Y Q , CHANG S K C , ZHANG Y .Comparison of α-amylase, α-glucosidase and lipase inhibitory activity of the phenolic substances in two black legumes of different genera[J].Food Chemistry,2017,214,259-268.
16	AGUNLOYE O M , OBOH G .Caffeic acid and chlorogenic acid: Evaluation of antioxidant effect and inhibition of key enzymes linked with hypertension[J].Journal of Food Biochemistry,2018,42(4):1-10.
17	彭焱辉, 杨熙, 王志强, 等.分光光度法测定金樱子果中总皂苷的含量[J].山东化工,2019,48(16):117-119.
	PENG Y H , YANG X , WANG Z Q , et al.Determination of total saponins in Rosa laevigata fruit by spectrophotometry[J].Shandong Chemical Industry,2019,48(16):117-119.
18	LIU S Y , AI Z Y , QU F F , et al.Effect of steeping temperature on antioxidant and inhibitory activities of green tea extracts against α-amylase, α-glucosidase and intestinal glucose uptake[J].Food Chemistry,2017,234,168-173.
19	SARIKURKCU C , KIRKAN B , OZER M S , et al.Chemical characterization and biological activity of Onosma gigantea extracts[J].Industrial Crops & Products,2018,115,323-329.
20	XIE P J , HUANG L X , ZHANG C H , et al.Reduced pressure extraction of oleuropein from olive leaves(Olea europaea L.) with ultrasound assistance[J].Food and Bioproducts Processing,2015,93,29-38.
21	NATASA M, PREDRAG K, MARIJANA S, et al. Kinetic modelling of ultrasound-assisted extraction of phenolics from cereal brans[J/OL]. Ultrasonics Sonochemistry, 2021, 79: 105761[2021-12-20]. https://doi.org/10.1016/j.ultsonch.2021.105761.
22	HU T , GUO Y Y , ZHOU Q F , et al.Optimization of ultrasonic-assisted extraction of total saponins from Eclipta prostrasta L.using response surface methodology[J].Journal of Food Science,2012,77(9):C975-C982.
23	AKBARI S , ABDURAHMAN N H , YUMUS R M .Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool[J].Comptes Rendus-Chimie,2019,22(11/12):714-727.
24	HADIDI M, IBARZ A, PAGAN J. Optimisation and kinetic study of the ultrasonic-assisted extraction of total saponins from alfalfa(Medicago sativa ) and its bioaccessibility using the response surface methodology[J/OL]. Food Chemistry, 2020, 309: 125786[2021-12-20]. https://doi.org/10.1016/j.foodchem.2019.125786.
25	LI J , ZU Y G , FU Y J , et al.Optimization of microwave-assisted extraction of triterpene saponins from defatted residue of yellow horn(Xanthoceras sorbifolia Bunge.) kernel and evaluation of its antioxidant activity[J].Innovative Food Science and Emerging Technologies,2010,11(4):637-643.
26	ZHENG Y X, TIAN J H, YANG W H, et al. Inhibition mechanism of ferulic acid against α-amylase and α-glucosidase[J/OL]. Food Chemistry, 2020, 317: 126346[2021-12-20]. https://doi.org/10.1016/j.foodchem.2020.126346.

来源 sources	平方和 sum of squares	自由度 df	均方 mean square	F值 F value	P值 P value
模型model	15.98	9	1.78	185.12	< 0.000 1^**
X₁	1.80	1	1.80	187.41	< 0.000 1^**
X₂	0.115 4	1	0.115 4	12.04	0.010 4^*
X₃	0.084 3	1	0.084 3	8.78	0.021 0^*
X₁X₂	0.005 0	1	0.005 0	0.518 2	0.494 9
X₁X₃	0.068 9	1	0.068 9	7.18	0.031 5^*
X₂X₃	0.820 8	1	0.820 8	85.59	< 0.000 1^**
X₁²	9.40	1	9.40	980.62	< 0.000 1^**
X₂²	1.37	1	1.37	142.79	< 0.000 1^**
X₃²	1.30	1	1.30	135.62	< 0.000 1^**
残差residual	0.067 1	7	0.009 6
失拟项lack of fit	0.045 0	3	0.015 0	2.71	0.179 7
纯误差pure error	0.022 1	4	0.005 5
总离差total	16.05	16

[1]	詹梦茹, 王彦斌, 康淑荷, 罗兴平. 党参多糖的大孔树脂纯化及其美白、吸湿保湿性能[J]. 林产化学与工业, 2023, 43(3): 79-88.
[2]	张梅, 张燕杰, 陈为健, 井华芳, 娄本勇. 锦绣杜鹃花色苷微胶囊的制备及其稳定性研究[J]. 林产化学与工业, 2023, 43(2): 19-26.
[3]	颜洋洋, 张昌伟, 李川, 周昊, 王成章. 不同纯度靛蓝提取物的制备及其抑菌活性研究[J]. 林产化学与工业, 2023, 43(1): 104-112.
[4]	付佳, 吴禹践, 刘志明, 王海英. 桑叶乙醇提取物化学成分分析及提取工艺优化[J]. 林产化学与工业, 2023, 43(1): 120-126.
[5]	陈默然, 于华忠, 刘磊磊, 唐克华. 漂洗降低白及militarine含量的响应面法研究[J]. 林产化学与工业, 2022, 42(6): 117-122.
[6]	罗路, 罗凌聪, 邓剑平, 周亚兰, 范毜仔, 赵伟刚. 杉木树皮基活性炭的制备、表征及其电化学性能研究[J]. 林产化学与工业, 2022, 42(5): 37-44.
[7]	王淑捷, 侯贤锋, 黄颖诗, 陈小凤, 孙瑾, 高振忠. 碱性离子液体TBAH预处理对桉木结构和酶解性能的影响[J]. 林产化学与工业, 2022, 42(1): 57-63.
[8]	郁峰, 王志宏, 张光耀, 杜志云, 彭密军. 天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性[J]. 林产化学与工业, 2022, 42(1): 101-109.
[9]	黄天, 张晓彤, 赵江琳, 郭健铭, 周鑫, 徐勇. 木糖酸辅助水解木聚糖制备低聚木糖及其分离与回收工艺研究[J]. 林产化学与工业, 2021, 41(5): 8-14.
[10]	陈淑琴, 叶燕燕, 全颖萱, 李林海, 倪林, 邹双全. 圆齿野鸦椿籽油提取工艺及脂肪酸组分分析[J]. 林产化学与工业, 2021, 41(1): 53-59.
[11]	蒋一苇, 郭娜, 刘志国, 赵春建, 付玉杰, 王希清. 蓝靛果籽油提取工艺优化及其脂肪酸组成分析[J]. 林产化学与工业, 2020, 40(6): 30-36.
[12]	张爱华,唐娟,赖鹏英,何怡丹,黎继烈,肖志红. 光皮梾木油热解产物催化加氢制备生物燃料[J]. 林产化学与工业, 2020, 40(5): 43-49.
[13]	张婷婷,韩秀丽,刘莹,董春玲,常春. 生物质基活性炭对环丙沙星的吸附性能研究[J]. 林产化学与工业, 2020, 40(4): 71-78.
[14]	陈亮,詹桂萍,鲁颖,李加兴,余佶,麻成金,姚茂君. 杜仲籽油脂质体的制备及其稳定性研究[J]. 林产化学与工业, 2020, 40(1): 68-76.
[15]	吕喆璇,张威伟,刘丹丹,朱莉伟,蒋建新. 肥皂荚皂苷提取工艺优化及其表面张力研究[J]. 林产化学与工业, 2019, 39(6): 102-108.