HPLC-DAD法分析不同月份和干燥方式下银杏叶色素含量的变化规律

doi:10.3969/j.issn.0253-2417.2023.02.012

林产化学与工业 ›› 2023, Vol. 43 ›› Issue (2): 89-97.doi: 10.3969/j.issn.0253-2417.2023.02.012

HPLC-DAD法分析不同月份和干燥方式下银杏叶色素含量的变化规律

沈红¹^,²^,³(), 张昌伟¹^,², 陈虹霞¹^,², 袁花¹^,², 蒋建新³, 王成章¹^,²^,*()

1. 中国林业科学研究院林产化学工业研究所; 江苏省生物质能源与材料重点实验室; 国家林业和草原局林产化学工程重点实验室; 林木生物质低碳高效利用国家工程研究中心, 江苏南京 210042
2. 南京林业大学江苏省林业资源高效加工利用协同创新中心, 江苏南京 210037
3. 北京林业大学材料科学与技术学院, 北京 100083

收稿日期:2021-12-24 出版日期:2023-04-28 发布日期:2023-04-26
通讯作者: 王成章 E-mail:2978963446@qq.com;wangczlhs@sina.com
作者简介:王成章, 研究员, 博士生导师, 主要从事天然产物研究与利用; E-mail: wangczlhs@sina.com
沈红(1992-), 女, 河南信阳人, 博士生, 从事天然产物的研究工作; E-mail: 2978963446@qq.com

Change Law of Pigments Content of Ginkgo biloba Leaves in the Different Months and Dry Modes Analyzed by HPLC-DAD Method

Hong SHEN¹^,²^,³(), Changwei ZHANG¹^,², Hongxia CHEN¹^,², Hua YUAN¹^,², Jianxin JIANG³, Chengzhang WANG¹^,²^,*()

1. Institute of Chemical Industry of Forest Products, CAF; Key Lab. of Biomass Energy and Material, Jiangsu Province; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Nanjing 210042, China
2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China
3. College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China

Received:2021-12-24 Online:2023-04-28 Published:2023-04-26
Contact: Chengzhang WANG E-mail:2978963446@qq.com;wangczlhs@sina.com

摘要/Abstract

摘要：

采用HPLC-DAD法研究了不同月份银杏叶色素含量的变化规律, 并通过聚类分析和逼近理想值排序(TOPSIS)法评价了不同干燥方式对银杏叶色素含量的影响。研究结果表明：所建立的HPLC-DAD方法简单、准确、灵敏度高、重复性好；4~11月银杏叶色素含量存在明显差异, 其中脱植基叶绿素a、脱镁叶绿酸盐a和β-胡萝卜素在4月份达到最高值, 分别为(1 762±121)、(628±32)和(482±45) μg/g, 叶绿素a和b在5月份达到最高值, 分别为(8 701±571)和(3 140±274) μg/g, 叶黄素在7月份达到最高值, 为(2 057±104) μg/g。聚类分析结果表明：12种干燥方式可分为4类, 冷冻干燥、40 ℃减压干燥、40 ℃常压干燥聚为A类, 阴干聚为B类, 100 ℃减压干燥和100 ℃常压干燥聚为D类, 剩下6种干燥方式聚为C类。通过TOPSIS法分析发现: 银杏叶干燥方式排名前5的依次为冷冻干燥、40 ℃减压干燥、40 ℃常压干燥、阴干和60 ℃常压干燥。综合考虑, 40 ℃减压干燥是最适合于工业化生产的干燥方法, 干燥速度快, 且色素得到有效保护。

关键词: HPLC-DAD, 银杏叶, 色素, 干燥方式, 变化规律

Abstract:

HPLC-DAD method was used to study the change law of content of Ginkgo biloba leaves pigments in different months, and the effects of various drying methods on the content of G.biloba leaves pigments were evaluated by cluster analysis and technique for order preference by similarity to an ideal solution(TOPSIS). The results showed that the established HPLC-DAD method was simple, accurate, sensitive and reproducible. G. biloba leaves pigments content was significantly different from April to November. The contents of chlorophylide a, pheophorbidel a and β-carotene reached the highest values in April, which were (1 762±121), (628±32) and (482±45) μg/g, respectively. The contents of chlorophyll a and b arrived at the highest values in May, which were (8 701±571) and(3 140±274) μg/g, respectively. The content of lutein could get to the highest value in July, which was (2 057±104) μg/g. Cluster analysis results demonstrated that 12 kinds of drying methods could be divided into four categories. Freeze drying, vacuum drying at 40℃, and atmospheric drying at 40℃ were clustered as A category. Shade drying was clustered as B category. vacuum drying at 100℃ and atmospheric drying at 100℃ were clustered as D category. The left 6 kinds of drying methods were clustered as C category. Through the analysis of TOPSIS method, it was found that the top five drying methods of G. biloba leaves were freeze drying, vacuum drying at 40℃, atmospheric drying at 40℃, shade drying and atmospheric drying at 60℃. Considering comprehensively, vacuum drying at 40℃ was the most suitable drying method for industrial production, with fast drying speed and effective protection of pigments.

Key words: HPLC-DAD, Ginkgo biloba leaves, pigments, drying modes, change law

中图分类号:

TQ35
TQ041

沈红, 张昌伟, 陈虹霞, 袁花, 蒋建新, 王成章. HPLC-DAD法分析不同月份和干燥方式下银杏叶色素含量的变化规律[J]. 林产化学与工业, 2023, 43(2): 89-97.

Hong SHEN, Changwei ZHANG, Hongxia CHEN, Hua YUAN, Jianxin JIANG, Chengzhang WANG. Change Law of Pigments Content of Ginkgo biloba Leaves in the Different Months and Dry Modes Analyzed by HPLC-DAD Method[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 89-97.

图/表 7

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编号 No.	干燥方式¹⁾ drying methods	压强/kPa pressure	温度/℃ temperature	时间/h time
S1		-100	40	6
S2	减压干燥 vacuum drying	-100	60	4
S3		-100	80	2
S4		-100	100	1
S5			40	12
S6	常压干燥 atmospheric drying		60	4
S7			80	2
S8			100	1
S9	冷冻干燥freeze drying		-50	24
S10	杀青减压干燥^* blanching vacuum drying	-100	60	4
S11	晒干sun drying		25-35	12
S12	阴干shade drying		25-30	120

序号 No.	组分 component	回归方程 regression equation	相关系数 correlation coefficient	线性范围/ (mg·L^-1) linear range	检出限/ (mg·L^-1) LOD	定量限/ (mg·L^-1) LOQ
1	叶绿素a chlorophyll a	Y=73 697X+90 179	0.999 2	4.688-75	0.156	0.468
2	脱镁叶绿素a pheophytin a	Y=125 106X+78 017	0.999 4	2.5-40	0.133	0.400
3	脱植基叶绿素a chlorophyllide a	Y=69 897X+20 103	0.999 2	0.625-10	0.042	0.125
4	脱镁叶绿酸盐a pheophorbide a	Y=110 345X+1 282	0.999 6	1.563-25	0.052	0.156
5	叶绿素b chlorophyll b	Y=114 304X+89 782	0.999 4	1.563-25	0.052	0.156
6	脱镁叶绿素b pheophytin b	Y=170 481X+4 866	0.999 9	1.25-20	0.042	0.125
7	叶黄素lutein	Y=86 820X+10 182	0.999 7	1.563-25	0.052	0.156
8	α-胡萝卜素 α-carotene	Y=130 310X+10 627	0.999 3	0.625-10	0.042	0.125
9	β-胡萝卜素 β-carotene	Y=118 310X+10 627	0.999 8	0.625-10	0.068	0.246

月份 month	脱植基叶绿素a chlorophyllide a	脱镁叶绿酸盐a pheophorbide a	叶绿素b chlorophyll b	叶绿素a chlorophyll a	脱镁叶绿素a pheophytin a	叶黄素 lutein	α-胡萝卜素 α-carotene	β-胡萝卜素 β-carotene
4月Apr.	1 762±121	628±32	1 745±141	3 875±315	725±43	872±49	56±5	482±45
5月May	616±37	509±41	3 140±274	8 701±571	474±26	1 201±68	272±39	336±21
6月June	410±26	352±17	2 904±157	7 958±446	479±45	1 251±101	331±27	328±11
7月July	—	168±85	2 231±152	5 528±321	484±43	2 057±104	349±25	315±27
8月Aug.	—	159±12	1 696±154	3 974±378	486±41	865±83	332±31	314±32
9月Sep.	—	147±12	1 025±101	2 303±231	562±54	553±53	132±12	222±21
10月Oct.	—	—	1 005±98	2 194±212	803±78	467±43	86±7	218±20
11月Nov.	—	—	109±9	217±21	50±4	364±34	55±4	91±8

编号 No.	脱植基叶绿素a chlorophyllide a	脱镁叶绿酸盐a pheophorbide a	叶绿素b chlorophyll b	叶绿素a chlorophyll a	脱镁叶绿素b pheophytin b	脱镁叶绿素a pheophytin a	叶黄素 lutein	α-胡萝卜素 α-carotene	β-胡萝卜素 β-carotene
S1	120±11	305±29	2 840±281	7 010±689	216±23	2 120±213	1 256±126	319±30	312±31
S2	—	287±27	1 025±101	1 165±112	1 944±191	6 074±603	898±87	263±25	250±23
S3	—	283±28	1 175±115	1 014±104	1 374±132	6 207±621	685±69	256±25	269±26
S4	—	189±19	501±46	426±43	848±79	3 335±321	278±28	113±12	124±13
S5	98±8	301±28	2 645±261	6 819±687	126±12	2 111±209	1 056±106	301±32	309±31
S6	—	293±27	1 037±101	1 342±132	1 968±187	6 203±632	924±93	283±27	282±27
S7	—	280±26	1 165±112	1 004±98	1 270±124	6 107±613	585±57	205±21	224±23
S8	—	180±15	478±34	435±42	809±78	3 039±301	250±26	97±11	76±8
S9	280±23	330±32	2 904±278	8 012±802	—	479±48	1 253±123	330±32	319±32
S10	—	75±6	—	—	2 020±201	6 068±609	703±71	55±5	63±6
S11	—	273±25	1 236±121	1 345±132	1 975±178	6 009±589	889±89	270±28	265±27
S12	—	295±27	1 624±15	3 300±331	1 567±154	4 045±408	1 114±113	295±30	281±27

编号 No.	正理想解距离 positive ideal solution distance(D⁺)	负理想解距离 negative ideal solution distance(D^-)	相对接近度 relative proximity(C_i)	排序结果 ranking results
S1	0.681	0.972	0.588	2
S2	1.080	0.692	0.390	7
S3	1.097	0.619	0.361	8
S4	1.247	0.299	0.194	11
S5	0.746	0.890	0.544	3
S6	1.072	0.715	0.400	5
S7	1.113	0.568	0.338	9
S8	1.264	0.271	0.176	12
S9	0.547	1.298	0.704	1
S10	1.268	0.561	0.307	10
S11	1.064	0.705	0.399	6
S12	0.990	0.716	0.420	4

HPLC-DAD法分析不同月份和干燥方式下银杏叶色素含量的变化规律

Change Law of Pigments Content of Ginkgo biloba Leaves in the Different Months and Dry Modes Analyzed by HPLC-DAD Method

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