超声波辅助类芬顿预处理增强桉木酶解效果

doi:10.3969/j.issn.0253-2417.2023.02.013

摘要/Abstract

摘要：

为克服传统芬顿体系用于预处理存在的问题, 构建了超声波辅助载铁沸石类芬顿(UZF)体系, 并采用单因素法, 确定UZF预处理桉木的较优条件, 即H₂O₂用量4%、超声波功率240 W、处理时间90 min，在此条件下, 桉木粉酶解产生的还原糖高达(435.12±7.69) mg/g。通过组成成分分析，以及傅里叶变换红外光谱(FT-IR)、X射线衍射(XRD)和扫描电镜(SEM)表征，探究了UZF预处理增强桉木酶解效率的机理, 分析了UZF预处理前后桉木样品的主要成分及基团、结晶性能和表面微观结构的变化，结果表明：预处理去除了更多木质素和半纤维素，使纤维素的无定形区破坏程度加大，木粉表面孔洞变多且更为粗糙。载铁沸石具有一定的循环使用性能, 在循环使用4次后, 仍能达到未处理桉木粉还原糖产量的1.87倍。

关键词: 超声波, 载铁沸石, 类芬顿, 桉木, 酶解

Abstract:

To overcome problems in traditional Fenton pretreatment, an ultrasonic-assisted iron-loaded zeolite Fenton-like(UZF) system was constructed, and single factor method was used to determine optimal conditions for UZF pretreatment of eucalyptus, i.e., H₂O₂ concentration of 4%, ultrasonic power of 240 W, and treatment time of 90 min. Under these conditions, the reducing sugar produced by enzymatic enzymolysis of eucalyptus powder was as high as (435.12±7.69) mg/g. The mechanism of UZF pretreatment to enhance enzymolysis efficiency of eucalyptus was investigated by means of composition, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy(SEM). The changes of main components and groups, crystalline properties, and surface microstructure of eucalyptus samples before and after UZF pretreatment were analyzed. The results showed that pretreatment removed more lignin and hemicellulose, which increased destruction of amorphous area of cellulose, and surface holes of wood powder became more and surface was more rough. The experimental results of recycling showed that the iron-loaded zeolite had certain recycling ability. After four times of recycling, reducing sugar yield could still reach 1.87 times than that of untreated eucalyptus powder.

Key words: ultrasonic, iron-loaded zeolite, fenton-like, eucalyptus, enzymolysis

中图分类号:

TQ35

陈梦婕, 王志男, 王淑捷, 黄颖诗, 侯贤锋, 高振忠. 超声波辅助类芬顿预处理增强桉木酶解效果[J]. 林产化学与工业, 2023, 43(2): 98-104, 126.

Mengjie CHEN, Zhinan WANG, Shujie WANG, Yingshi HUANG, Xianfeng HOU, Zhenzhong GAO. Ultrasound-assisted Fenton-like Pretreatment of Eucalyptus for Enhancing Enzymolysis[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 98-104, 126.

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样品sample	O	Al	Si	Ca	Fe
天然沸石natural zeolite	65.22	6.38	26.32	1.75	0.32
载铁沸石iron-loaded zeolite	58.89	10.52	28.35	0.38	1.87

条件conditions		还原糖产量/(mg·g^-1) reducing sugar yield
H₂O₂用量 H₂O₂ dosage	2%	421.10
	4%	435.12
	6%	373.33
超声波功率 ultrasonic power	160 W	391.00
	240 W	435.12
	320 W	365.25
时间 time	50 min	411.45
	90 min	435.12
	130 min	293.37

桉木木粉¹⁾eucalyptus powder	纤维素cellulose/%	半纤维素hemicellulose/%	木质素lignin/%
未处理untreated	41.35±1.61	26.31±2.22	27.79±0.70
UAF预处理UAF pretreated	44.98±0.70	22.41±3.20	27.03±0.45
UZF预处理UZF pretreated	45.52±0.99	25.32±0.55	23.15±1.11

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