三元低共熔溶剂预处理甘蔗渣的酶解性能研究

doi:10.3969/j.issn.0253-2417.2023.02.017

摘要/Abstract

摘要：

以制糖工业副产物甘蔗渣(SCB)作为木质纤维原料, 使用具有优异相转移催化能力的三乙基苄基氯化铵(TEBAC)、无毒无害的甘油(GL)，以及高价态路易斯酸六水合氯化铝(ACH)组成的三元低共熔溶剂(DES)对SCB进行预处理, 系统考察了DES物质的量之比、预处理温度、预处理时间、固液质量比对SCB中各组分含量和纤维素酶解效率的影响。研究结果表明：最佳预处理条件为n_TEBAC∶n_GL∶n_ACH为1∶2∶0.05、预处理时间30 min、预处理温度120 ℃和固液质量比1∶15, 此优化条件下木质素去除率达到(86.23±2.11)%, 纤维素保留率为(94.51±2.03)%, 酶解纤维素转化率达到(98.21±1.02)%, 相较于未处理SCB提高了2倍, 葡萄糖得率高达(81.94±1.98)%, 提高了1.5倍。SEM、FT-IR和XRD分析结果表明：三元DES预处理能够有效去除SCB中的木质素和半纤维素, 从而使纤维素的结晶度由未预处理的41.19%提高到预处理后的65.87%。DES经过5次循环利用后, 纤维素转化率和葡萄糖得率仍达到(92.36±1.64)%和(74.45±1.12)%, 依旧保持了较好的预处理效果。

关键词: 三元低共熔溶剂, 甘蔗渣, 预处理, 酶解

Abstract:

Sugarcane bagasse(SCB), a by-product of the sugar industry, was used as a lignocellulosic raw material. In the present study, a ternary deep eutectic solvent(DES) composed of triethylbenzyl ammonium chloride(TEBAC) with strong phase transfer catalytic ability, non-toxic and harmless glycerol(GL) and high-valence Lewis acid aluminum chloride hexahydrate(ACH), was used to pretreat SCB. The effects of molar ratio of ternary DES, reaction temperature, reaction time and solid-liquid mass ratio on the content of each component in SCB and cellulase enzymatic digestibility were systematically investigated. The results showed that the optimal pretreatment conditions were as follows: the molar ratio of n_TEBAC: n_GL: n_ACH was 1:2:0.05, the pretreatment time was 30 min, the pretreatment temperature was 120℃ and the solid-liquid ratio was 1:15. Under these conditions, the lignin removal rate was (86.23±2.11)%, and the cellulose retention rate was (94.51±2.03)%. The conversion of enzymatic hydrolysis cellulose was (98.21±1.02)%, which was 2 times higher than that of untreated SCB, and the yield of glucose was(81.94±1.98)%, which was increased by 1.5 times. The results of SEM, FT-IR and XRD analysisshowed that ternary DES pretreatment could effectively remove lignin and hemicellulose from SCB, and the crystallinity of celluloseincreased from 41.19% to 65.87% after pretreatment. Importantly, DES still maintained good pretreatment performance after 5cycles of recycling, and the cellulose conversion rate and glucose yield reached (92.36±1.64)% and (74.45±1.12)%, respectively.

Key words: ternary deep eutectic solvent, sugarcane bagasse, pretreatment, enzymatic digestibility

中图分类号:

TQ35

陶顺辉, 张可炫, 李碧莹, 郑晓洁, 刘耀, 林晓清. 三元低共熔溶剂预处理甘蔗渣的酶解性能研究[J]. 林产化学与工业, 2023, 43(2): 135-142.

Shunhui TAO, Kexuan ZHANG, Biying LI, Xiaojie ZHENG, Yao LIU, Xiaoqing LIN. Enzymatic Digestibility of Ternary Deep Eutectic Solvent Pretreated Sugarcane Bagasse[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 135-142.

图/表 5

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项目 item		残渣回收率/% residue recovery	残渣组成residue composition/%
项目 item		残渣回收率/% residue recovery	纤维素 cellulose	木聚糖 xylan	木质素 lignin
n_TEBAC∶n_GL∶n_ACH	未处理untreated	100	37.72±1.06	22.95±1.32	22.34±1.42
	1∶2∶0	99.06±1.20	30.02±1.12	18.25±0.91	20.55±1.32
	1∶2∶0.01	53.31±2.61	62.51±1.21	9.43±0.61	7.48±0.86
	1∶2∶0.05	42.61±2.36	82.97±1.13	2.90±0.19	6.92±0.65
	1∶2∶0.1	42.50±1.78	76.43±1.54	0.22±0.11	5.47±0.21
	1∶2∶0.2	40.23±1.98	77.96±1.21	0.79±0.12	6.15±0.33
	1∶2∶0.3	39.78±1.92	74.82±1.54	0.58±0.21	10.28±0.45
	ACH	91.85±1.68	46.98±1.32	20.57±0.12	21.26±1.02
预处理温度 pretreatment temperature	80 ℃	75.95±2.01	49.17±1.36	20.21±1.05	19.36±0.98
	100 ℃	53.03±1.96	68.34±1.57	12.47±1.02	8.76±0.78
	120 ℃	47.02±2.10	82.97±1.45	2.90±0.96	6.92±1.02
	140 ℃	39.44±1.83	86.23±1.11	3.03±0.87	10.18±0.96
预处理时间 pretreatment time	10 min	47.22±1.89	75.35±2.31	5.48±0.52	5.98±0.85
	30 min	44.77±1.48	79.67±2.12	4.64±0.39	6.99±0.96
	60 min	47.02±1.72	82.97±1.99	2.90±0.48	6.92±0.74
	90 min	42.94±1.99	83.15±2.03	4.42±0.56	7.08±0.85
固液比(g∶g) solid-to-liquid ratio	1∶5	48.20±1.89	73.54±1.56	14.72±0.98	7.50±0.58
	1∶10	46.22±1.96	78.22±1.85	13.54±1.21	8.30±0.92
	1∶15	44.77±2.02	79.67±1.91	4.64±0.67	6.99±0.54
	1∶20	42.86±2.11	77.17±2.04	7.38±1.11	2.43±0.21

项目 item		纤维素保留率/% cellulose retention rate	木聚糖损失率/% xylan loss rate	木质素去除率/% lignin removal rate	纤维素转化率/% cellulose conversion	葡萄糖得率/% glucose yield
n_TEBAC∶n_GL∶n_ACH	未处理untreated	100	0	0	32.57±2.10	32.57±2.21
	1∶2∶0	78.85±2.02	21.23±2.10	8.84±1.01	14.20±1.20	8.47±1.46
	1∶2∶0.01	88.35±1.95	78.09±1.59	82.15±2.65	99.18±0.06	79.00±1.36
	1∶2∶0.05	93.74±2.04	94.62±2.31	86.79±2.33	98.99±0.09	81.71±2.14
	1∶2∶0.1	86.12±1.89	99.59±0.40	89.59±2.51	99.39±0.40	79.13±2.06
	1∶2∶0.2	83.14±2.01	98.61±1.31	88.93±2.63	94.59±1.20	67.15±1.90
	1∶2∶0.3	78.91±2.22	99.00±0.65	81.69±2.22	99.20±0.60	73.18±1.64
	ACH	99.00±0.98	17.91±1.22	12.83±1.32	30.68±1.62	34.99±0.94
预处理温度 pretreatment temperature	80 ℃	98.99±0.63	33.10±1.01	34.18±1.21	67.16±2.46	62.28±2.63
	100 ℃	96.08±1.97	71.18±2.10	79.20±2.10	82.72±1.69	75.83±1.56
	120 ℃	93.74±1.23	94.62±2.56	86.79±2.41	98.99±0.68	81.71±2.33
	140 ℃	90.16±2.15	94.79±1.65	82.03±1.66	99.61±0.03	83.33±2.48
预处理时间 pretreatment time	10 min	95.99±2.12	85.82±0.98	86.93±2.63	83.93±2.22	79.14±2.33
	30 min	94.25±2.01	91.81±1.36	86.23±1.56	98.21±1.63	81.94±2.41
	60 min	93.74±1.89	94.62±2.13	86.79±1.36	98.99±0.59	81.71±2.68
	90 min	94.65±1.64	91.73±1.89	86.38±2.13	94.83±2.76	75.38±2.17
固液比(g∶g) solid-to-liquid ratio	1∶5	95.85±1.02	72.72±1.20	83.82±2.10	84.81±2.31	79.69±2.44
	1∶10	94.25±2.01	91.81±1.42	82.82±2.51	85.31±2.45	81.77±1.69
	1∶15	94.51±2.03	85.14±1.11	86.23±2.11	98.21±1.02	81.94±1.98
	1∶20	90.16±1.98	94.79±2.51	95.33±1.69	91.89±2.12	80.57±2.46

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