刺玫果提取物中总三萜酸的定性定量分析研究

doi:10.3969/j.issn.0253-2417.2015.05.022

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (5): 135-142.doi: 10.3969/j.issn.0253-2417.2015.05.022

刺玫果提取物中总三萜酸的定性定量分析研究

钟方丽¹, 王晓林¹, 杨扬^1,2

1. 吉林化工学院化学与制药工程学院, 吉林吉林 132022;
2. 吉林大学化学学院, 吉林长春 130012

收稿日期:2014-09-16 出版日期:2015-10-25 发布日期:2015-10-24
通讯作者: 王晓林(1969—),男,山东五莲人,副教授,硕士,主要从事天然产物有效成分的提取、纯化及药物的研究与开发;E-mail:wangxiaolin69@eyou.com E-mail:wangxiaolin69@eyou.com
作者简介:钟方丽(1970—),女,山东安丘人,教授,博士,硕士生导师,主要从事天然产物化学成分的分离与生物活性研究;E-mail:fanglizhong@sina.com
基金资助:
吉林省教育厅资助项目(吉教科合字[2013] 第318号)

Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.

ZHONG Fang-li¹, WANG Xiao-lin¹, YANG Yang^1,2

1. School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;
2. School of Chemistry, Jilin University, Changchun, 130012, China

Received:2014-09-16 Online:2015-10-25 Published:2015-10-24

摘要/Abstract

摘要： 建立刺玫果提取物中总三萜酸的定性定量分析方法。采用 TLC 法对刺玫果提取物总三萜酸中的熊果酸进行定性分析;采用UV法对刺玫果提取物中的总三萜酸进行了定量分析;采用HPLC法对刺玫果提取物总三萜酸中的齐墩果酸、熊果酸进行了定量分析。结果表明,UV法中齐墩果酸在1.75~15.75 mg/L范围内与吸光度有良好的线性关系(r=0.999 1),平均加样回收率为101.60% (RSD 1.23%);HPLC法中齐墩果酸、熊果酸在0.1~1.1 μg(r=0.999 7)、0.024~0.768 μg(r=0.999 9) 线性关系良好,平均加样回收率分别为100.24% (RSD 1.44%)、100.54%(RSD 0.45%)。3批刺玫果提取物中总三萜酸平均为8.62%,齐墩果酸平均为0.273%,熊果酸平均为0.534%。结果表明,所建立的刺玫果提取物中总三萜酸的定性分析和定量分析方法可用于该提取物的质量控制。

关键词: 刺玫果, 总三萜酸, 齐墩果酸, 熊果酸

Abstract: The qualitative and quantitative analysis methods of total triterpenoid acids (TTA) in fruit extracts of Rosa davurica Pall. were established. Both UV and HPLC methods were applied to determine the content of TTA, oleanolic acid and ursolic acid. TLC method was used to accomplish the qualitative identification of ursolic acid in fruit extracts. The concentration of oleanolic acid showed good linear relationship with absorbance at the range of 1.75-15.75 mg/L (r=0.999 1) and the average recovery was 101.60% (RSD 1.23%) in quantitative analysis of TTA with UV method. On the other hand, oleanolic acid and ursolic acid showed good linear relationship at the range of 0.1-1.1 μg (r=0.999 7) and 0.024-0.768 μg (r=0.999 9) respectively and the average recoveries of them were 100.24%(RSD 1.44%) and 100.54%(RSD 0.45%) by HPLC method. The average contents of TTA, oleanolic acid, and ursolic acid of three batches of fruit extracts were 8.62%, 0.273%, and 0.534%, respectively. The experimental results showed that the content determination and TLC qualitative identification methods established could be applied to control of the qualitative and quantitative analysis of fruit extracts of R.davurica.

Key words: fruit of Rosa davurica Pall., total triterpenoid acids, oleanolic acid, ursolic acid

中图分类号:

TQ35

钟方丽, 王晓林, 杨扬. 刺玫果提取物中总三萜酸的定性定量分析研究[J]. 林产化学与工业, 2015, 35(5): 135-142.

ZHONG Fang-li, WANG Xiao-lin, YANG Yang. Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 135-142.

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刺玫果提取物中总三萜酸的定性定量分析研究

Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.

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