酶水解对马尾松纤维细胞壁结构及微纤丝制备的影响

doi:10.3969/j.issn.0253-2417.2017.03.011

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (3): 82-88.doi: 10.3969/j.issn.0253-2417.2017.03.011

酶水解对马尾松纤维细胞壁结构及微纤丝制备的影响

朱晶航^1,2, 李新平^1,2, 王转^1,2, 杜敏^1,2, 陈立红³

1. 陕西科技大学轻工科学与工程学院, 陕西西安 710021;
2. 陕西省造纸技术及特种纸品开发重点实验室, 陕西西安 710021;
3. 陕西科技大学化学与化工学院, 陕西西安 710021

收稿日期:2016-10-24 出版日期:2017-06-25 发布日期:2017-07-03
通讯作者: 李新平,教授,博士,博士生导师,主要研究方向为纤维素功能材料、植物资源高值化利用;E-mail:lixp@sust.edu.cn E-mail:lixp@sust.edu.cn
作者简介:朱晶航(1990-),男,陕西西安人,硕士生,主要从事纤维素功能性材料的研究
基金资助:
国家自然科学基金资助项目（31370578）；陕西省科技厅自然科学基础研究面上项目（2015JM3083）；陕西省科技厅省级重点实验室项目（2011HBSZS014）

Effects of Enzymatic Hydrolysis on Cell Wall Structure of Masson Pine and Preparation of Microfibrillated Cellulose

ZHU Jinghang^1,2, LI Xinping^1,2, WANG Zhuan^1,2, DU Min^1,2, CHEN Lihong³

1. College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China;
2. Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, Xi'an 710021, China;
3. College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China

Received:2016-10-24 Online:2017-06-25 Published:2017-07-03

摘要/Abstract

摘要： 漂白马尾松纤维经纤维素酶水解后，用高压均质机处理得到纤维素微纤丝（MFC），利用快速比表面积及孔隙度分析仪、X射线衍射仪、红外光谱仪对MFC的聚合度、形貌、直径分布进行分析，探究酶水解对制备MFC的促进作用。结果表明：酶水解作用能够改变纤维的比表面积、结晶度和孔隙结构。漂白马尾松纤维在100 MPa压力下均质处理30次后得到的MFC的聚合度为354，直径主要分布在20~50 nm；漂白马尾松纤维在50 ℃下经纤维素酶（酶用量10.0 FPU/g）水解2 h，在100 MPa压力下均质处理30次后得到的MFC的聚合度为229，直径主要分布在10~40 nm。

关键词: 酶水解, 孔隙分布, 结晶度, 比表面积, MFC

Abstract: The microfibrillated cellulose(MFC) was prepared from cellulose hydrolyzed masson pine fibers by high-pressure homogenization. The properties of enzymatic hydrolyzed fibers were characterized by surface area and porosity analyzer, X-ray diffractometer and Fourier transform infrared spectrometer. The effect of enzymatic hydrolysis on the preparation of MFC was investigated based on the analysis of the degree of polymerization (D_P), morphology and diameter distribution of MFC. The results showed that the specific surface area, crystallinity and pore structure of fibers could be changed by enzymatic hydrolysis. As the fiber were homogenized 30 times under 100 MPa, the D_P and diameter distribution of MFC obtained from raw fibers were 354 and 20-50 nm respectively, while the D_P and diameter distribution of MFC obtained from enzymatic fibers which was hydrolyzed by 10 FPU/g cellulase at 50℃ for 2 h were 229 and 10-40 nm respectively.

Key words: enzyme pretreatment, pore distribution, crystallinity, specific surface area, microfibrillated cellulose

中图分类号:

TQ35

朱晶航, 李新平, 王转, 杜敏, 陈立红. 酶水解对马尾松纤维细胞壁结构及微纤丝制备的影响[J]. 林产化学与工业, 2017, 37(3): 82-88.

ZHU Jinghang, LI Xinping, WANG Zhuan, DU Min, CHEN Lihong. Effects of Enzymatic Hydrolysis on Cell Wall Structure of Masson Pine and Preparation of Microfibrillated Cellulose[J]. Chemistry and Industry of Forest Products, 2017, 37(3): 82-88.

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酶水解对马尾松纤维细胞壁结构及微纤丝制备的影响

Effects of Enzymatic Hydrolysis on Cell Wall Structure of Masson Pine and Preparation of Microfibrillated Cellulose

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