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林产化学与工业 ›› 2018, Vol. 38 ›› Issue (1): 73-80.doi: 10.3969/j.issn.0253-2417.2018.01.011

• 研究报告 • 上一篇    下一篇

隐孔菌中倍半萜类化合物及其抗氧化活性研究

周凌云1,2, 鲁斌1, 余小红1, 张明芮2, 华燕1   

  1. 1. 西南林业大学 西南山地森林资源保育与利用教育部重点实验室, 云南 昆明 650224;
    2. 皖南医学院 安徽省多糖药物工程技术研究中心, 安徽 芜湖 241002
  • 收稿日期:2017-02-26 出版日期:2018-02-25 发布日期:2018-02-28
  • 通讯作者: 华燕,教授,博士生导师,主要从事天然药物化学研究;E-mail:huayan1216@163.com。 E-mail:huayan1216@163.com
  • 作者简介:周凌云(1980-),男,安徽芜湖人,博士生,研究方向为天然药物化学
  • 基金资助:
    西南林业大学科技创新基金资助(C15045,C15049);皖南医学院中青年科研基金资助(WK201610)

Antioxidant Activity of Sesquiterpenes from Cryptoporus volvatus (Peck) Hubbard

ZHOU Lingyun1,2, LU Bin1, YU Xiaohong1, ZHANG Mingrui2, HUA Yan1   

  1. 1. Southwest Forestry University, Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Kunming 650224, China;
    2. School of Pharmacy, Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wuhu 241002, China
  • Received:2017-02-26 Online:2018-02-25 Published:2018-02-28

摘要: 通过清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基法、2,2'-连氨-二(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基法、水杨酸法和邻苯三酚自氧化法,测定隐孔菌碱提物、乙酸乙酯提取物、60%乙醇提取物和水提醇沉物4个粗提部分的抗氧化活性,结果显示:乙酸乙酯提取物清除总自由基能力最强,其对ABTS自由基半数清除率(IC50)达到(67.43±1.41)mg/L。进一步对乙酸乙酯提取物进行活性追踪分离,得到9个倍半萜类化合物:隐孔酸A三甲酯(1),隐孔酸A(2),隐孔酸D四甲酯(3),隐孔酸D(4),隐孔酸C(5),隐孔酸C五甲酯(6),隐孔酸E五甲酯(7),隐孔酸B三甲酯(8)和隐孔酸B(9),其中化合物1368为首次从该种真菌中分离得到。化合物1~9对ABTS自由基的清除能力测定结果显示化合物8有较高抗氧化活性,其(IC50=(5.29±0.25)mg/L,清除能力优于阳性对照VC,VC的IC50=(5.76±0.58)mg/L。

关键词: 隐孔菌, 倍半萜酸类, 抗氧化活性, 活性追踪分离, 构效关系

Abstract: The antioxidant activities of four extracts (alkali extracts,ethyl acetate extract,60% ethanol extract and crude polysaccharide) from Cryptoporus volvatus(Peck) Hubbard were evaluated by methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical,2,2'-azino-di-(3-ethyl-2,3-dihydrobenzthiazoline-6-sulfonate)(ABTS),Salicylic acid,and Fenton reaction.The results indicated that ethyl acetate extract showed the strongest free radical scavenging ability with IC50=(67.43±1.41) mg/L by the ABTS method.Further study by bioassay-guided fractionation of the ethyl acetate extract led to the isolation and identification of nine cryptoporic compounds including cryptoporic acid A trimethyl ester (1),cryptoporic acid A (2),cryptoporic acid D tetra-methyl ester (3),cryptoporic acid D (4),cryptoporic acid C (5),cryptoporic acid C pentamethyl ester (6),cryptoporic acid E pentamethyl ester (7),cryptoporic acid B trimethyl ester (8) and crytoporic acid B (9).Among them,compounds 1,3,6,8 were isolated for the first time from this fungus.The results of the ABTS scavenging ability showed that compound 8(cryptoporic acid B trimethyl ester) has a higher antioxidant activity with IC50=(5.29±0.25) mg/L than the positive control VC with IC50=(5.76±0.58) mg/L.In addition,the structure-relationship of sesquiterpenes as antioxidant agents was analyzed.

Key words: Cryptoporus volvatus, sesquiterpenes, antioxidant activities, bioassay-guided fractionation, structure-activity relationship

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