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林产化学与工业 ›› 2018, Vol. 38 ›› Issue (5): 85-92.doi: 10.3969/j.issn.0253-2417.2018.05.012

• 研究报告 • 上一篇    下一篇

杜仲叶总多酚超声波-微波辅助提取及其抗氧化活性研究

王翔1,2, 彭胜1, 彭密军2   

  1. 1. 吉首大学 杜仲综合利用技术国家地方联合工程实验室;林产化工工程湖南省重点实验室, 湖南 张家界 427000;
    2. 广东省测试分析研究所 广东省化学危害应急检测技术重点实验室, 广东 广州 510070
  • 收稿日期:2018-06-26 出版日期:2018-10-25 发布日期:2018-11-06
  • 通讯作者: 彭密军,教授,硕士生导师,研究领域为天然产物开发与利用;E-mail:pengmj163@163.com。 E-mail:pengmj163@163.com
  • 作者简介:王翔(1993-),男,浙江杭州人,硕士生,主要从事天然产物开发与利用研究;E-mail:66419130@qq.com
  • 基金资助:
    国家自然科学基金资助项目(31660181);杜仲综合利用技术国家地方联合工程实验室项目(NLE201602&NLE201704);湖南省教育厅重点项目(16A175)

Extraction of Total Polyphenols from Eucommia ulmoides Oliver Leaves Assisted by Ultrasound-microwave and Their Antioxidant Activity

WANG Xiang1,2, PENG Sheng1, PENG Mijun2   

  1. 1. National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Key Laboratory of Human Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
    2. Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Institute of Analysis, Guangzhou 510070, China
  • Received:2018-06-26 Online:2018-10-25 Published:2018-11-06

摘要: 采用超声波-微波辅助法优化杜仲叶中总多酚提取工艺,并考察其抗氧化活性。通过Plackett-Burman试验考察了乙醇体积分数、料液比、微波功率、微波处理时间、超声波功率和超声波处理时间6个因素对杜仲叶总多酚提取效果的影响,筛选得到乙醇体积分数、料液比、微波功率和超声波处理时间4个显著影响因素,在此基础上,通过最陡爬坡试验和响应面试验得到最佳提取条件为:乙醇体积分数为46%,料液比为1:20.70(g:mL),微波功率为154W,微波处理时间140s,超声波功率350W,超声波处理时间为31 min。在此条件下提取2次总多酚得率达8.491%,与传统溶剂提取法相比,总多酚得率提高33.57%。利用最优工艺得到总多酚提取物,其体外抗氧化试验表明:杜仲叶总多酚提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、2,2-联氮-二-3-乙基-苯并噻唑-6-磺酸自由基(ABTS·)和羟基自由基(·OH)均有较强的清除能力,其半数抑制质量浓度(IC50)分别为31.21、24.50和311.8mg/L,抗氧化活性与BHT相当,低于Vc。

关键词: 超声波-微波辅助提取, Plackett-Burman设计, 响应面, 总多酚, 抗氧化

Abstract: The extraction technology of total polyphenols from Eucommia ulmoides Oliver leaves was optimized by ultrasound-microwave assisted method and their antioxidant activity were investigated. Experiments were conducted to investigate the effects of 6 factors, such as ethanol volume fraction, solid-liquid ratio, ultrasound power, ultrasound extraction time, microwave power, and microwave extraction time on the yield of total polyphenols from E. ulmoides leaves by Plackett-Burman design. After screening the four significant factors including ethanol volume fraction, solid-liquid ratio, microwave power and ultrasound extraction time, the process optimization was carried out by the steepest ascent design and response surface. The optimal extraction conditions were as follows:ethanol volume fraction 46%, solid-liquid ratio 1:20.70(g:mL), microwave power 154 W, microwave extraction time 140 s, ultrasound power 350 W and ultrasound extraction time 31 min. Under this condition, the yield of total polyphenols was 8.491%, which was improved by 33.57% compared with the traditional method. The extract of total polyphenols has been obtained by optimal process and the results of antioxidant assay in vitro showed that the extract of total polyphenols from E. ulmoides leaves had obvious scavenging capacity for DPPH·, ABTS·and·OH, with the inhibitory concentration 50% (IC50) values of 31.21, 24.50 and 311.8 mg/L respectively, and the antioxidant activity is equivalent to BHT and lower than Vc.

Key words: Ultrasound-microwave assisted extract, Placket-Burman design, response surface, total polyphenols, antioxidant activity

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