大孔树脂纯化白及多酚及其体外抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2019.04.013

摘要/Abstract

摘要：

对比了10种大孔树脂对白及多酚粗提液的静态吸附与解吸效果，并对优选的LSA-21树脂纯化白及多酚粗提液的工艺进行了探讨，对纯化前后的白及多酚提取液的体外抗氧化活性进行检测。研究结果表明：室温下，多酚粗提液质量浓度0.4~0.6 g/L，pH值4，上样流速2.0 mL/min为LSA-21树脂吸附白及多酚粗提液的最佳工艺；乙醇体积分数80%，解吸液pH值6，上样流速1.0 mL/min为最佳解吸工艺。以100 mL质量浓度为0.47 g/L粗提液在最佳工艺条件下纯化，白及多酚纯度由4.48%提高到27.61%，纯化效果较好。纯化后的白及多酚清除自由基能力有显著提升，其对DPPH自由基、羟基自由基和超氧阴离子自由基的半数抑制浓度（IC₅₀）分别为7.72、8.65和205.39 mg/L。

关键词: 白及, 多酚, 大孔树脂, 纯化, 抗氧化

Abstract:

The static adsorption and desorption effects of ten kinds of macroporous resins on B. striata polyphenols crude extract were compared, and the optimal process of purifying with LSA-21 resin was discussed. Furthermore, the antioxidant activities of the pre- and post-purification B. striata polyphenols were tested in vitro. The results showed that, at room temperature, the optimal process parameters for adsorption of crude extract with LSA-21 resin were as follows:the mass concentration of polyphenol crude extract 0.4-0.6 g/L, pH vlaue 4, and the loading velocity 2.0 mL/min. The optimal process parameters for desorption were as follows:desorption solution ethanol volume fraction 80%, pH vlaue 6, and elution flow rate 1.0 mL/min. The purity increased from 4.48% to 27.61% after purifying 100 mL of 0.47g/L crude extract under the optimal technological conditions, and the purification effect was good. The DPPH·scavenging activity of the purified B. striata polyphenols was obviously improved. The IC₅₀ of DPPH·、OH· and O₂^-·free radicals were 7.72, 8.65 and 205.39 mg/L respectively.

Key words: Bletilla striata, polyphenols, macroporous resin, purification, antioxidation

中图分类号:

TQ35
R284.2

彭焱,唐克华,董爱文. 大孔树脂纯化白及多酚及其体外抗氧化活性研究[J]. 林产化学与工业, 2019, 39(4): 91-99.

Yan PENG,Kehua TANG,Aiwen DONG. Purification of Bletilla striata Polyphenols by Macroporous Resin and Its Antioxidant Activities in Vitro[J]. Chemistry and Industry of Forest Products, 2019, 39(4): 91-99.

图/表 9

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树脂型号 resin model	颗粒外观 particle appearance	极性 polarity	粒径/mm granularity	吸附率/% adsorption rate	解吸率/% desorption rate
AB-8	乳白色不透明球状milk white opaque sphere	弱极性week polar	0.315~1.250	51.32±1.44^bc	71.41±2.77^f
DM130	乳白色不透明球状milk white opaque sphere	中极性moderately polar	0.300~1.250	41.75±1.1^e	96.76±2.92^a
LSA-10	乳白色不透明球状milk white opaque sphere	中极性moderately polar	0.300~1.200	47.89±0.5^d	77.98±0.78^e
LSA-21	米黄色不透明球状beige opaque sphere	中极性moderately polar	0.315~1.250	52.20±0.84^b	92.34±1.39^b
LX-28	乳白色不透明球状milk white opaque sphere	中极性moderately polar	0.315~1.250	52.36±1.36^b	85.68±1.63^c
XDA-6	乳白色不透明球状milk white opaque sphere	中极性moderately polar	0.315~1.250	57.95±0.69^a	85.11±1.76^c
LKA98Cl	乳白色不透明球状milk white opaque sphere	极性polar	0.315~1.250	35.68±1.11^f	54.14±1.87^g
LS-303	米黄色不透明球状beige opaque spher	极性polar	0.315~1.250	57.31±0.41^a	83.41±0.53^cd
LK20	乳白色不透明球状milk white opaque sphere	非极性nonpolar	0.300~1.200	58.51±1.27^a	79.42±2.92^de
D-312	乳白色不透明球状milk white opaque sphere	非极性nonpolar	0.300~1.200	49.73±0.77^c	80.89±1.96^de

指标 indicators	多酚 polyphenol	DPPH·清除能力 DPPH·scavenging ability	OH·清除能力 OH·scavenging ability	O₂^-·清除能力 O₂^-·scavenging ability	总抗氧化能力 total antioxidant ability
多酚 polyphenol	1.000	0.946^**	0.939^**	0.814^*	0.991^**
DPPH·清除能力 DPPH·scavenging ability		1.000	0.983^**	0.946^**	0.914^*
OH·清除能力 OH·scavenging ability			1.000	0.955^**	0.926^**
O₂^-·清除能力 O₂^-·scavenging ability				1.000	0.790
总抗氧化能力 total antioxidant ability					1.000

指标 indicators	多酚 polyphenol	DPPH·清除能力 DPPH· scavenging ability	OH·清除能力 OH· scavenging ability	O₂^-·清除能力 O₂^-· scavenging ability	总抗氧化能力 total antioxidant ability
多酚 polyphenol	1.000	0.961^**	0.957^**	0.639	0.996^**
DPPH·清除能力 DPPH· scavenging ability		1.000	0.990^**	0.786	0.967^**
OH·清除能力 OH·scavenging ability			1.000	0.789	0.970^**
O₂^-·清除能力 O₂^-·scavenging ability				1.000	0.696
总抗氧化能力 total antioxidant ability					1.000

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