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林产化学与工业

林产化学与工业 ›› 2021, Vol. 41 ›› Issue (3): 33-39.doi: 10.3969/j.issn.0253-2417.2021.03.005

• 重点研发专栏 • 上一篇    下一篇

酶法制备桃叶珊瑚苷元及其稳定性研究

曹金腾1,2, 吴涛1, 董玉蓉1, 仇聪1, 姜艳1, 赵林果1,*()   

  1. 1. 南京林业大学 南方现代林业协同创新中心, 江苏 南京 210037
    2. 南京林业大学 化学工程学院, 江苏 南京 210037
  • 收稿日期:2020-09-21 出版日期:2021-06-28 发布日期:2021-07-01
  • 通讯作者: 赵林果 E-mail:lgzhao@njfu.edu.cn
  • 作者简介:赵林果, 教授, 博士生导师, 研究领域为林源活性物的开发利用、天然药物生物催化与转化; E-mail: lgzhao@njfu.edu.cn
    曹金腾(1997-), 女, 河北沧州人, 硕士生, 主要从事天然药物生物催化与转化研究工作
  • 基金资助:
    国家重点研发计划资助项目(2016YFD0600805)

Enzymatic Preparation of Aucubigenin and Its Stability

Jinteng CAO1,2, Tao WU1, Yurong DONG1, Cong QIU1, Yan JIANG1, Linguo ZHAO1,*()   

  1. 1. Co-Innovation Center for Sustainbale Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
    2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2020-09-21 Online:2021-06-28 Published:2021-07-01
  • Contact: Linguo ZHAO E-mail:lgzhao@njfu.edu.cn

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摘要：

以桃叶珊瑚苷为原料，利用β-葡萄糖苷酶水解制备桃叶珊瑚苷元，单因素试验优化了单水相和正丁醇-水双相体系制备桃叶珊瑚苷元的酶解条件；研究了影响桃叶珊瑚苷元稳定性的主要因素。研究结果表明：单水相酶法催化适宜条件为温度35℃、时间20 min、酶用量为25 U/mL，此条件下桃叶珊瑚苷元产率达46.3%。通过筛选构建了正丁醇-水双相反应体系，双相酶解适宜的条件为正丁醇体积分数80%、温度35℃、酶用量为25 U/mL、时间20 min，此条件下苷元产率达87.9%。桃叶珊瑚苷元热稳定性较差，在-20和0℃条件下12 h内非常稳定；在中性和碱性条件下极易分解，在pH值3.0时较为稳定，8 h内仅降解12%；添加5 g/L VC的苷元水溶液稳定性得到显著改善，2 h内仅降解8%；苷元在正丁醇中比较稳定，8 h内几乎不降解。

关键词: 双相体系, β-葡萄糖苷酶, 稳定性, 桃叶珊瑚苷

Abstract:

Aucubigenin was prepared from aucubin by β-glucosidase hydrolysis. The single water and n-butanol-water two-phase enzymatic hydrolysis conditions of aucubigenin were optimized bysingle factor experiment. The main factors that affected the stability of aucubigenin were investigated.The results showed that the optimum conditions of single-phase enzymatic hydrolysis were temperature 35℃, time 20 min, enzyme dosage 25 U/mL, and the yield of aucubigenin reached 46.3%. Through screening, the n-butanol-water two-phase reaction system was constructed. The optimum conditions of two-phase enzymatic hydrolysis were n-butanol volume fraction 80%, temperature 35℃, enzyme dosage 25 U/mL, time 20 min, and the yield of aglycone reached 87.9%.Aucubigenin had poor thermal stability, and it was relatively stable in 12 h at-20 and 0℃; it was easy to decompose under neutral and alkaline conditions, and was most stable at pH 3.0, only 12% degraded in 8 h; the stability of the aqueous solution of aucubigenin with 5 g/L VC was significantly improved, only 8% degraded within 2 h; aucubigenin was very stable in n-butanol and hardly degraded in 8 h.

Key words: two-phase system, β-glucosidase, stability, aucubin

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