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林产化学与工业 ›› 2015, Vol. 35 ›› Issue (1): 45-50.doi: 10.3969/j.issn.0253-2417.2015.01.007

• 研究报告 • 上一篇    下一篇

非水相体系中木瓜脂肪酶催化合成萜烯醇芳香酯的研究

苏二正1, 尤鹏永2, 魏东芝2   

  1. 1. 南京林业大学 轻工科学与工程学院, 酶与发酵工程实验室, 江苏 南京 210037;
    2. 华东理工大学 生物反应器工程国家重点实验室, 鲁华生物技术研究所, 上海 200237
  • 收稿日期:2014-02-25 出版日期:2015-02-25 发布日期:2015-03-05
  • 作者简介:苏二正(1976-),男,安徽巢湖人,副教授,博士,主要从事工业酶与生物催化方向的研究工作;E-mail:ezhsu@njfu.edu.cn。
  • 基金资助:
    南京林业大学引进高层次人才科研基金资助(G2014024);南京林业大学"青年拔尖人才培养计划"专项经费资助(无编号);江苏高校优势学科建设工程资助项目(无编号)

Catalytic Synthesis of Terpene Alcohol Aromatic Ester with Carica papaya Lipase in Non-aqueous System

SU Er-zheng1, YOU Peng-yong2, WEI Dong-zhi2   

  1. 1. Enzyme & Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2. New World Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2014-02-25 Online:2015-02-25 Published:2015-03-05

摘要: 为建立经济、绿色、高效的萜烯醇芳香酯合成工艺,以木瓜蛋白酶粗品为原料制备获得了天然固定化木瓜脂肪酶(CPL),研究了CPL在非水相体系中催化合成萜烯醇芳香酯的效果,探讨了在有机溶剂体系中萜烯醇种类、有机溶剂种类以及初始水分活度对反应的影响。结果表明:伯醇类萜烯醇(香茅醇和香叶醇)是较适宜的底物,中等极性的有机溶剂如正己烷为适宜的反应溶剂,0.328的初始水分活度有利于CPL发挥催化活性,此时转化率为95.2%,与商业脂肪酶催化效果相当。CPL在有机溶剂中重复使用性较差,考察了不同离子液体处理方法对CPL催化性能的影响,结果表明,使用微量离子液体对CPL进行包裹前处理可以显著地提高CPL的重复使用性,反应15次后,催化活性仍保留90%以上。

关键词: 非水相体系, 木瓜脂肪酶, 萜烯醇, 芳香酯

Abstract: In order to establish economic, green and efficient synthesis process of terpene alcohol aromatic ester, naturally immobilized Carica papaya lipase (CPL) was prepared from the crude papain, and was used to catalyze the synthesis of terpene alcohol aromatic ester in non-aqueous system. The effects of terpene alcohol type, organic solvent type, and initial water activity on the CPL catalyzed reaction were investigated. The results showed that the primary terpene alcohols (geraniol and citronellol) were more suitable substrates, solvents with moderate polarity such as n-hexane were suitable reaction medium, and initial water activity of 0.328 benefitted the catalytic activity of CPL in organic solvents. Under these conditions, the substrate conversion rate was 95.2%, which was similar to those of commercial lipases. The reusability of CPL in organic solvent was poor. Three treatment methods by introduction of the ionic liquid were investigated on their effects on CPL catalyzed performance. It was found that wrapping the CPL with trace amount of ionic liquid before use could significantly enhance the CPL stability. The catalytic activity of CPL still remained more than 90% after being used in the organic solvent for 15 batches.

Key words: non-aqueous system, Carica papaya lipase, terpene alcohol, aromatic ester

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