植物细胞壁中甘露糖单元与木质素间化学键连接的研究

doi:10.3969/j.issn.0253-2417.2015.03.008

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (3): 43-48.doi: 10.3969/j.issn.0253-2417.2015.03.008

植物细胞壁中甘露糖单元与木质素间化学键连接的研究

石超群¹, 谢益民^1,2, 王鹏^1,2

1. 湖北工业大学制浆造纸工程学院, 湖北武汉 430068;
2. 湖北工业大学绿色轻工材料湖北省重点实验室, 湖北武汉 430068

收稿日期:2014-03-24 出版日期:2015-06-25 发布日期:2015-07-03
通讯作者: 谢益民,教授,博士生导师,主要从事木质素化学的研究;E-mail:ppymxie@scut.edu.cn。 E-mail:ppymxie@scut.edu.cn
作者简介:石超群(1988—),女,湖北仙桃人,硕士,主要从事植物纤维资源化学研究工作
基金资助:
国家自然科学基金资助项目(31070521, 31370574);湖北省教育厅青年人才项目(Q20131402)

Chemical Association between Mannose Units and Lignin in Plant Cell Wall

SHI Chao-qun¹, XIE Yi-min^1,2, WANG Peng^1,2

1. School of Pulping &Papermaking Engineering, Hubei University of Technology, Wuhan 430068, China;
2. Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan 430068, China

Received:2014-03-24 Online:2015-06-25 Published:2015-07-03

摘要/Abstract

摘要： 从甘露糖单元的角度分析半纤维素中糖单元与木质素苯丙烷结构单元之间的连接方式,从而阐明裸子植物中木质素-碳水化合物复合体(LCC)的木质素与半纤维素之间的相互关系。为了使生长中的植物细胞壁甘露糖单元上的碳原子被 ¹³C 同位素示踪并抑制甘露糖向木质素转化,将带D-¹³C₆-甘露糖、外源性木质素前驱物松伯醇-β-D-葡萄糖苷以及苯丙氨酸解氨酶的抑制剂AOPP一起投入到银杏(Ginkgo biloba L.)植株中。结果表明,新生木质部的LCC中甘露糖单元被 ¹³C 同位素标记,而且对银杏的正常代谢没有明显的影响。从带 ¹³C 标记的新生木质部组织提取LCC,并用纤维素酶和半纤维素酶酶解LCC得酶解LCC(EDLCC)。用FT-IR及 ¹³C NMR 分析LCC和EDLCC,结果证明半纤维素中的甘露糖C-6位与木质素侧链的α-C以苯甲醚键方式连接。

关键词: 木质素-碳水化合物复合体, 半纤维素, 甘露糖, ¹³C 示踪, 苯甲醚键

Abstract: In order to elucidate the chemical association between hemicellulose and lignin in gymnosperm, the linkage between sugar units in hemicellulose and phenylpropane substructure of lignin was analyzed from the perspective of mannose. The mannan precursor D-¹³C₆-mannose, exogenous lignin precursor coniferin, and the inhibitor of phenylalanine ammonia-lyase, were injected into a living ginkgo tree (Ginkgo biloba L.) to trace the mannose in the cell wall by ¹³C and meanwhile inhibit the conversion of mannose to lignin. The results indicate that the mannose units of lignin-carbohydrate complexes (LCC) in newly-formed xylem are labeled successfully by ¹³C isotope. Moreover, the metabolism of ginkgo tree has not been affected obviously by the addition of the precursors and inhibitor. The specifically ¹³C-enriched LCCs were isolated from the newly-formed xylem and further hydrolyzed by cellulase and hemicellulase to get enzyme-degraded LCCs (EDLCCs). The analyses of FT-IR and ¹³C NMR of EDLCCs indicate that the C-6 position of mannose unit in hemicellulose and α-carbon of lignin side chain is linked by benzyl ether bond.

Key words: lignin-carbohydrate complexes, hemicellulose, mannose, ¹³C tracer, benzyl ether linkage

中图分类号:

TQ35

石超群, 谢益民, 王鹏. 植物细胞壁中甘露糖单元与木质素间化学键连接的研究[J]. 林产化学与工业, 2015, 35(3): 43-48.

SHI Chao-qun, XIE Yi-min, WANG Peng. Chemical Association between Mannose Units and Lignin in Plant Cell Wall[J]. Chemistry and Industry of Forest Products, 2015, 35(3): 43-48.

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植物细胞壁中甘露糖单元与木质素间化学键连接的研究

Chemical Association between Mannose Units and Lignin in Plant Cell Wall

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