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林产化学与工业 ›› 2015, Vol. 35 ›› Issue (3): 91-96.doi: 10.3969/j.issn.0253-2417.2015.03.016

• 研究报告 • 上一篇    下一篇

造纸废弃物麦草废渣高温自水解预处理对酶水解性能的影响

黄萍1,2, 朱圆圆1, 徐勇1, 勇强1, 黄立新2, 余世袁1   

  1. 1. 南京林业大学 化学工程学院, 江苏 南京 210037;
    2. 中国林业科学研究院 林产化学工业研究所, 江苏 南京 210042
  • 收稿日期:2015-01-24 出版日期:2015-06-25 发布日期:2015-07-03
  • 通讯作者: 余世袁,教授,博士生导师,研究领域为林产资源生物化学加工利用。 E-mail:874700831@qq.com
  • 作者简介:黄萍(1982—),女,江苏张家港人,工程师,硕士生,主要从事生物化工的研究;E-mail:874700831@qq.com
  • 基金资助:
    “十二五”国家科技支撑计划资助项目(2015BAD15B09);江苏高校优势学科建设工程资助项目(无编号)

Effects of Hot-compressed Water on the Enzymatic Hydrolyzability of Papermaking Waste Wheat Straw

HUANG Ping1,2, ZHU Yuan-yuan1, XU Yong1, YONG Qiang1, HUANG Li-xin2, YU Shi-yuan1   

  1. 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2. Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China
  • Received:2015-01-24 Online:2015-06-25 Published:2015-07-03

摘要: 对造纸废弃物麦草废渣进行高温自水解预处理,并对预处理后的麦草废渣进行组成分析和酶解,以纤维素和木聚糖的酶解得率为考核指标,研究了不同自水解预处理条件对麦草废渣酶解的影响。研究表明,预处理后麦草废渣的化学组成和物理结构均发生了改变,纤维素的含量增加,比表面积增大且促进了纤维素的酶解。酶解结果显示,温度对预处理麦草废渣的酶水解得率有着极其显著的影响。以200 ℃自水解预处理60 min的麦草废渣为酶水解底物(质量浓度50 g/L),添加纤维素酶35 FPIU/g(以纤维素计,下同)、β-葡萄糖苷酶25 IU/g、木聚糖酶120 U/g及2 g/L聚乙二醇(PEG6000),酶解体系50 mL,于50 ℃酶解36 h,其纤维素和木聚糖酶解得率分别达86.29%和74.03%,是麦草废渣经170 ℃自水解40 min后酶解得率的2.3倍和2.8倍。

关键词: 麦草废渣, 高温自水解, 预处理, 酶解

Abstract: Wheat straw from papermaking waste was pretreated by hot-compressed water. The test of enzymatic hydrolysis and its compositions was studied.The effects of pretreatment conditions on waste wheat straw were investigated,and the enzymatic hydrolysis yields of cellulose and xylan were examined. Results showed that chemical composition of waste wheat straw and physical structures were changed after pretreatment,and the cellulose contents and surfaces increased.This promoted the enzymatic hydrolysis of cellulose.Enzymatic hydrolysis results showed that the temperature of pretreatment had a very significant impact on the yield.Waste wheat straw pretreated at 200 ℃ for 60min was used as the enzyme hydrolysis substrate at a concentration of 50 g/L.Under the conditions of cellulose enzyme 35 FPIU/g (of cellulose), β-glucose glycosides enzyme 25 IU/g,xylanase enzyme 120 U/g,PEG6000 2 g/L,enzymatic hydrolysis temperature 50 ℃, and reaction time 36 h,the hydrolysis yield of cellulose and xylan reached 86.29% and 74.03%,respectively.They improved 2.3 times and 2.8 times of those pretreated by 170 ℃ for 40 min.

Key words: waste wheat straw, hot-compressed water, pretreatment, enzymatic hydrolysis

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