不同菌种对油橄榄叶发酵饲料的影响

doi:10.3969/j.issn.0253-2417.2015.04.010

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (4): 59-64.doi: 10.3969/j.issn.0253-2417.2015.04.010

不同菌种对油橄榄叶发酵饲料的影响

谢普军^1,2, 黄立新^1,2, 张彩虹^1,2, 游凤^1,2, 张耀雷^1,2

1. 中国林业科学研究院林业新技术研究所, 北京 100091;
2. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2014-05-13 出版日期:2015-08-25 发布日期:2015-08-29
通讯作者: 黄立新(1967—),男,研究员,博士,博士生导师,研究领域为天然产物化学及新型干燥技术;E-mail:l_x_huang@163.com。 E-mail:l_x_huang@163.com
作者简介:谢普军(1986—),男,江西丰城人,博士生,主要研究方向为天然产物化学及固态发酵技术;E-mail:xpjdzh@163.com
基金资助:
中国林科院林业新技术所基本科研业务费专项资金(CAFINT2013C04);"十二五"国家科技支撑计划资助(2012BAD32B10)

Effect of Different Strains on Fermented Feedstuff from Olive Leaves

XIE Pu-jun^1,2, HUANG Li-xin^1,2, ZHANG Cai-hong^1,2, YOU Feng^1,2, ZHANG Yao-lei^1,2

1. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
2. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2014-05-13 Online:2015-08-25 Published:2015-08-29

摘要/Abstract

摘要： 以黑曲霉、白地霉、米曲霉、绿色木霉、产朊假丝酵母和热带假丝酵母为固态发酵菌种,考察单菌及混合菌种对油橄榄叶发酵饲料中纤维素酶活(β-葡萄糖苷酶活、羧甲基纤维素酶活及滤纸酶活),蛋白质及总单宁含量的影响。结果表明,单菌发酵效率最高的菌种为黑曲霉,发酵3天时纤维素酶活最高,β-葡萄糖苷酶活10.53 U/mL、羧甲基纤维素(CMC)酶活10.61 U/mL、滤纸(FPA)酶活4.02U/mL,发酵5天时蛋白质最高达14.61%,发酵7天总单宁降解率最高达84.14%;混菌发酵效率最高的为黑曲霉与产朊假丝酵母的组合,其最佳比例为1:1(体积比),发酵时间5天,纤维素酶活最高,β-葡萄糖苷酶活12.45 U/mL、CMC酶活12.59 U/mL及FPA酶活5.51 U/mL,发酵7天时蛋白质最高可达18.63%,总单宁降解率最高为86.49%。

关键词: 菌种, 油橄榄叶, 固态发酵, 纤维素酶活, 蛋白质含量, 总单宁含量

Abstract: Olive leaves were fermented by Aspergillus niger, Geotrichum candidum, Aspergillus oryzae, Candida utilis, Candida tropicalis, and Trichoderma viride. The effects of the single and the combination of two strains on the cellulase activity(β-glucosidase, CMCase and filter paper activity), protein content, and total tannins content were investigated. The results indicated that the best fermented efficiency for single strain of olive leaves fermented feedstuff was A.niger, with a result of β-glucosidase activity 10.53 U/mL, CMCase activity 10.61 U/mL and filter paper activity 4.02 U/mL when they were fermented for 3 days. The highest protein content 14.61% for 5 days and the highest tannins degradation 84.14% for 7 days were achieved. The best fermented efficiency by mixed strains of olive leaves fermented feedstuff was the combination of A. niger and C. utilis with a ratio of 1:1(v/v). The highest cellucase activity(β-glucosidase activity 12.45 U/mL, CMCase activity 12.59 U/mL, filter paper activity 5.51 U/mL) was obtained by the mixed strains(1:1) in need of 5 days. When the fermented time reached 7 days, the protein content reached 18.63% and total tannic degradation was 86.49% in the fermented product.

Key words: strains, olive leaves, solid state fermentation, cellulase activity, protein content, total tannins content

中图分类号:

TQ35
TQ920.9

谢普军, 黄立新, 张彩虹, 游凤, 张耀雷. 不同菌种对油橄榄叶发酵饲料的影响[J]. 林产化学与工业, 2015, 35(4): 59-64.

XIE Pu-jun, HUANG Li-xin, ZHANG Cai-hong, YOU Feng, ZHANG Yao-lei. Effect of Different Strains on Fermented Feedstuff from Olive Leaves[J]. Chemistry and Industry of Forest Products, 2015, 35(4): 59-64.

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不同菌种对油橄榄叶发酵饲料的影响

Effect of Different Strains on Fermented Feedstuff from Olive Leaves

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