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林产化学与工业 ›› 2015, Vol. 35 ›› Issue (6): 126-132.doi: 10.3969/j.issn.0253-2417.2015.06.021

• 研究报告 • 上一篇    下一篇

流苏花精油的超临界CO2萃取及抗菌活性研究

刘普, 李小方, 高嘉屿, 尹卫平, 邓瑞雪   

  1. 河南科技大学 河南省伏牛山野生药材基源工程技术研究中心, 化工与制药学院, 河南 洛阳 471023
  • 收稿日期:2014-10-09 出版日期:2015-12-25 发布日期:2015-12-28
  • 作者简介:刘普(1978—),男,河南邓州人,副教授,博士,主要从事植物天然活性成分的研究*通信作者:邓瑞雪,副教授,博士,研究方向为活性天然产物及其全合成;E-mail:dengliu20022002@aliyun.com。
  • 基金资助:
    国家自然科学基金-河南人才培养联合基金资助(U1404831)

Extraction of Essential Oil from the Flower of Chionanthus retusus Lindl.et Paxton by Supercritical CO2 and Its Antibacterial Activities

LIU Pu, LI Xiao-fang, GAO Jia-yu, YIN Wei-ping, DENG Rui-xue   

  1. Henan Engineering Technology Research Center for Funiu Mountain Wild Medicinal Source, Chemical Engineering & Pharmaceutical College, Henan University of Science and Technology, Luoyang 471023, China
  • Received:2014-10-09 Online:2015-12-25 Published:2015-12-28

摘要: 采用单因素和正交试验法讨论了超临界CO2流体法萃取流苏花精油的工艺条件,利用气相色谱-质谱(GC-MS)联用技术分析了精油的化学成分,并对精油的抑菌活性进行了评价。结果表明采用超临界CO2流体技术萃取流苏花精油的最佳工艺条件是:100 g流苏花粉末在萃取压力25 MPa,萃取温度 45 ℃,萃取时间 2.0 h,CO2流量22 kg/h,在最佳工艺条件下精油的得率为0.409%。从流苏花精油中共分离出60个峰,确定了其中的57种化合物,所鉴定成分占总馏出峰面积的96.18%。体外抑菌试验结果表明,流苏花精油对金黄色葡萄球菌、白葡萄球菌和大肠杆菌等菌株具有较好的抑制作用。

关键词: 流苏, 精油, 超临界萃取, 抗菌

Abstract: Single factor experiment and orthogonal experiment design were used to study the influences of extraction conditions on the yield of essential oil from the flower of Chionanthus retusus Lindl.et Paxton by supercritical CO2 method.The components of the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS).The in vitro antibacterial activity of the eessential oil was characterized.The optimum extraction parameters were established with the extraction pressure 25 MPa, extraction temperature 45 ℃, extraction time 2.0 h, and the fluent of CO2 22 kg/h.The yield of essential oil was up to 0.409%.Sixty compositions were separated.Among them, 57 components were identified and respresented 96.18% of the total detected constituents.The results of the antibacteria, i.e., assay showed that the essential oil displayed good antibacterial activity against the tested bacterial, i.e. Staphyloccocus aureu, Staphyloccocus albus, Escherichia coli.

Key words: Chionanthus retusus Lindl. et Paxton, essentyial oil, supercritical extraction, antibacterial activity

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