银杏叶聚戊烯醇纳米乳液联合抗生素抗菌活性研究

doi:10.3969/j.issn.0253-2417.2017.01.010

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (1): 81-86.doi: 10.3969/j.issn.0253-2417.2017.01.010

银杏叶聚戊烯醇纳米乳液联合抗生素抗菌活性研究

陶冉^1,2, 王成章^1,2, 叶建中², 周昊^1,2, 陈虹霞²

1. 中国林业科学研究院林业新技术研究所, 北京 100091;
2. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2016-01-06 出版日期:2017-02-25 发布日期:2017-02-25
通讯作者: 王成章,研究员,博士,博士生导师,主要从事天然产物研究与利用方面的工作:E-mail:wangczlhs@sina.com。 E-mail:wangczlhs@sina.com
作者简介:陶冉(1982-),男,江苏连云港人,助理研究员,博士,主要从事天然产物化学的研究;E-mail:trmoon1949@163.com
基金资助:
中国林科院林业新技术所基本科研业务费专项资金（CAFINT2014C10）；江苏省自然科学青年基金项目（BK20150070）；江苏省林业三新工程项目（LYSX[2014]09）

Antibacterial Activity of Ginkgo biloba Leaves Polyprenols Nanoemulsion in Combination with Antibiotics

TAO Ran^1,2, WANG Chengzhang^1,2, YE Jianzhong², ZHOU Hao^1,2, CHEN Hongxia²

1. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
2. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2016-01-06 Online:2017-02-25 Published:2017-02-25

摘要/Abstract

摘要： 通过探讨银杏叶聚戊烯醇纳米乳液（GBP）的平均粒径对其自身抗菌增效作用的影响，以及考察最小抑菌浓度（MIC）和分级抑菌浓度（FIC）；同时研究亚抑菌浓度条件下，聚戊烯醇纳米乳液与抗生素联合作用的时间-杀菌曲线，研究了银杏叶聚戊烯醇纳米乳液与氨苄西林（A）、环丙沙星（C）、硫酸庆大霉素（G）、红霉素（E）和多粘菌素B硫酸盐（P）这5种抗生素的协同抗菌作用。结果显示：聚戊烯醇乳液配伍抗生素的抑菌圈直径随着乳液平均粒径增大而减小，由此推断聚戊烯醇纳米乳液可能具有提高聚戊烯醇自身的增效能力。聚戊烯醇纳米乳液与硫酸庆大霉素混合对金黄色葡萄球菌具有协同抗菌作用（FIC指数为0.5），混合样抑菌圈值为（25.8±0.1）mm，MIC值为33 mg/L。聚戊烯醇与硫酸庆大霉素混合可以有效地提高对金黄色葡萄球菌的抗菌效果并延长抗菌时间。

关键词: 银杏, 聚戊烯醇, 纳米乳液, 抗生素, 抗菌作用

Abstract: The synergistic antibacterial effects of Ginkgo biloba leaves polyprenols nanoemulsion (GBP) combined with five types of antibiotics,i.e., ampicillin (A), ciprofloxacin (C), gentamicin sulfate(G), erythromycin(E), and polymyxin B sulfate (P), were determined through analyzing the average diameter of the inhibition halos, minimal inhibitory concentration (MIC), fractional inhibitory concentration (FIC) index and the time-killing curves under sub-MIC conditions. The results showed that the antibacterial effect of polyprenols nanoemulsion combined with the antibiotics enhanced with the increase of the average diameter of polyprenols nanoemulsion. The antibacterial effect of polyprenols could be improved by nanoemulsion. The polyprenol combined with gentamicin sulfate had synergistic antibacterial effect against Staphylococcus aureus with the FIC index of 0.5, the diameter of the inhibition halos of (25.8±0.1)mm, and the MIC value of 33.0 mg/L. The time-killing curves showed that the antibacterial effect and the antibacterial time could be increased after polyprenols mixed with gentamicin sulfate.

Key words: Ginkgo biloba, polyprenol, nanoemulsion, antibiotic, antibacterial activity

中图分类号:

TQ35

陶冉, 王成章, 叶建中, 周昊, 陈虹霞. 银杏叶聚戊烯醇纳米乳液联合抗生素抗菌活性研究[J]. 林产化学与工业, 2017, 37(1): 81-86.

TAO Ran, WANG Chengzhang, YE Jianzhong, ZHOU Hao, CHEN Hongxia. Antibacterial Activity of Ginkgo biloba Leaves Polyprenols Nanoemulsion in Combination with Antibiotics[J]. Chemistry and Industry of Forest Products, 2017, 37(1): 81-86.

参考文献

[1] CHOJNACKI T,SWIEZEWSKA E,VOGTMAN T.Polyprenols from plants-structural analogues of mammalian dolichols[J].Chemica Scripta,1987(27):209-214.
[2] PRONIN A V,DANILOV L L,NAROVLYANSKY A N,et al.Plant polyisoprenoids and control of cholesterol level[J].Archivum Immunologiae et Therapiae Experimentalis,2014,62(1):31-39.
[3] 王成章,沈兆邦,谭卫红,等.银杏叶聚戊烯醇的化学、纯化和药效研究[J].天然产物研究与开发,2001,13(2):43-45. WANG C Z,SHEN Z B,TAN W H,et al.The studies on the chemistry,purification and pharmacology of polyprenols from Ginkgo biloba L.[J].Natural Product Research and Development,2001,13(2):43-45.
[4] YANG L, WANG C Z, YE J Z,et al.Hepatoprotective effects of polyprenols from Ginkgo biloba L.leaves on CCl₄-induced hepatotoxicity in rats[J].Fitoterapia,2011,82:834-840.
[5] FEDOTOVA I O,SULTANOV V S,KUZNETSOVA N N,et al.Effect of new polyprenol drug ropren on anxiety-depressive-like behavior in rats with experimental Alzheimer disease[J].Eksperimental'naia I Klinicheskaia Farmakologiia,2010,73(9):2-5.
[6] TAO R,WANG C Z,YE J Z,et al.Antibacterial/antifungal activity and synergistic interactions between C70-C120 polyprenol homologs from Ginkgo biloba L.leaves and the corresponding synthetic derivatives[J].European Food Research and Technology,2014,239(4):587-594.
[7] 王成章,王婉苓,叶建中,等.分子短程蒸馏分离银杏叶聚戊烯醇的研究[J].林产化学与工业,2008,28(3):23-27. WANG C Z,WANG W L,YE J Z,et al.Studies on seperation of polyprenols from leaves of Ginkgo biloba L.by molecular short distillation[J].Chemistry and Industry of Forest Products,2008,28(3):23-27.
[8] 陶冉,王成章,张宇思,等.银杏叶聚戊烯醇纳米乳液的制备研究[J].生物质化学工程,2015,49(1):7-12. TAO R,WANG C Z,ZHANG Y S,et al.Preparation of polyprenols nanoemulsion from Ginkgo biloba L.leaves polyprenols[J].Biomass Chemical Engineering,2015,49(1):7-12.
[9] TAO R, WANG C Z, KONG Z W.Antibacterial/antifungal activity and synergistic interactions between polyprenols and other lipids isolated from Ginkgo biloba L.leaves[J].Molecules,2013,18(2):2166-2182.
[10] SHANG R F,WANG G H,XU X M,et al.Synthesis and biological evaluation of new pleuromutilin derivatives as antibacterial agents[J].Molecules,2014,19(11):19050-19065.
[11] HALL M J,MIDDLETON R F,WESTMACOTT D.The fractional inhibitory concentration (FIC) index as a measure of synergy[J].Journal of Antimicrobial Chemotherapy,1983,11(5):427-433.
[12] GUTIERREZ J,BARRY-RYAN C,BOURKE P.Antimicrobial activity of plant essential oils using food model media:Efficacy,synergistic potential and interactions with food components[J].Food Microbiology,2009,26(2):142-150.
[13] VINCENT H T,SAMER K,GIAO V,et al.Comparative pharmacodynamics of gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa[J].Antimicrob Agents and Chemotherapy,2006,50(8):2626-2631.
[14] HUANG Q R,YU H L,RU Q M.Bioavailability and delivery of nutraceuticals using nanotechnology[J].Journal of Food Science,2010,75(1):50-57.
[15] BYRON F B,ERIC A J.Sensitization of Staphylococcus aureus and Escherichia coli to antibiotics by the sesquiterpenoids nerolidol,farnesol,bisabolol,and apritone[J].Antimicrob Agents and Chemotherapy,2003,47(10):3357-3360.
[16] CORNWELL P A,BARRY B W.Sesquiterpene components of volatile oils as skin penetration enhancers for the hydrophilic permeant 5-fluorou-racil[J].Journal of Pharmacy and Pharmacology,1994,46(4):261-269.
[17] CIEPICHAL E,JEMIOLA-RZEMINSKA M,HERTEL J,et al.Configuration of polyisoprenoids affects the permeability and thermotropic properties of phospholipids/polyisoprenoid model membranes[J].Chemistry & Physics of Lipids,2011,164(4):300-306.
[18] KADURUGAMUWA J L,CLARKE A J,BEVERIDGE T J.Surface action of gentamicin on Pseudomonas aeruginosa[J].Journal of Bacteriology,1993,175(18):5798-5805.

银杏叶聚戊烯醇纳米乳液联合抗生素抗菌活性研究

Antibacterial Activity of Ginkgo biloba Leaves Polyprenols Nanoemulsion in Combination with Antibiotics

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