小远志中口山酮类化学成分及生物活性研究

doi:10.3969/j.issn.0253-2417.2017.02.016

摘要/Abstract

摘要： 以小远志干燥根茎为原料，采用乙醇提取、硅胶柱层析、Sephadex LH-20、MCI-gel和半制备型HPLC分离等方法对小远志中口山酮类化学成分进行研究。从中分离得到18个（口山）酮类化合物，根据理化性质及ESI-MS、¹H NMR、¹³C NMR等波谱数据，分别鉴定为：1，7-二羟基（口山）酮（1），3，6-二羟基-1，2，7，8-四甲氧基（口山）酮（2），1，7-二羟基-5，6-二甲氧基（口山）酮（3），1，5-二羟基-6，7-二甲氧基（口山）酮（4），6-羟基-1，7-二甲氧基（口山）酮（5），7-羟基-1-甲氧基（口山）酮（6），3，8-二羟基-1，2，4-三甲氧基（口山）酮（7），6，8-二羟基-1，2，3-三甲氧基（口山）酮（8），1，3，6-三羟基-2，7，8-三甲氧基（口山）酮（9），3-羟基-1，2，8-三甲氧基（口山）酮（10），1，3，7-三羟基（口山）酮（11），1，6-二羟基-2，3-二氧亚甲基-7，8-二甲氧基（口山）酮（12），1，7-二羟基-2，3-二甲氧基（口山）酮（13），1，6-二羟基-5，7-二甲氧基（口山）酮（14），3，7-二羟基-1，2-二甲氧基（口山）酮（15），3，7-二羟基-1，2，8-三甲氧基（口山）酮（16），1，2，3，7-四甲氧基（口山）酮（17），1，6-二羟基-3，5，7，8-四甲氧基（口山）酮（18），除化合物13外，其它化合物均为首次从该植物中分离得到。对其中分离所得量较多的化合物1~7进行了抗氧化及抗菌实验，发现化合物4和1有较好的抗氧化活性，对ABTS·的IC₅₀值分别为12和13 mg/L；化合物3对金黄色葡萄球菌有一定的抑制作用，MIC值为217 μmol/L。

关键词: 小远志, （口山）酮类化学成分, 抗氧化, 抗菌

Abstract: Eighteen compounds were isolated by ethanol extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, MCI-gel column chromatography and semi-preparative HPLC from the dried roots of Polygala sibirica L. var. megalopha Fr. Their structures were identified as 1, 7-dihydroxy xanthone (1), 3, 6-dihydroxy-1, 2, 7, 8-tetramethoxy xanthone (2), 1, 7-dihydroxy-5, 6- dimethoxy xanthone (3), 1, 5-dihydroxy-6, 7-dimethoxy xanthone (4), 6-hydroxy-l, 7-dimethoxy xanthone (5), 7-hydroxy-1-methoxy xanthone (6), 3, 8-dihydroxy- 1, 2, 4-trimethoxy xanthone (7), 6, 8-dihydroxy-1, 2, 3-trimethoxy xanthone (8), 1, 3, 6-trihydroxy-2, 7, 8-trimethoxy- xanthone (9), 3-hydroxy-1, 2, 8-trimethoxy xanthone (10), 1, 3, 7-trihydroxy xanthone (11), 1, 6-dihydroxy-2, 3-methylene-dioxy-7, 8-dimethoxy xanthone (12), 1, 7-dihydroxy-2, 3-dimethoxy xanthone (13), 1, 6-dihydroxy-5, 7-dimethoxy xanthone (14), 3, 7-dihydroxy-1, 2-dimethoxy xanthone (15), 3, 7- dihydroxy- 1, 2, 8-trimethoxy xanthone (16), 1, 2, 3, 7-tetramethoxy xanthone (17), 1, 6-dihydroxy-3, 5, 7, 8-tetramethoxy xanthone (18) by ESI-MS, ¹H NMR, ¹³C NMR. All compounds except 13 were isolated from this plant for the first time. The antioxidant and antimicrobial activities of compound 1-7 were studied. And the results showed that the compound 4(IC₅₀=12 mg/L) and compound 1(IC₅₀=13 mg/L) showed good ABTS scavenging activity and compound 3(MIC=217 μmol/L) had certain inhibitory activity against Staphylococcus aureus.

Key words: Polygala. sibirica L. var. megalopha Fr., xathones, antioxidant, antimicrobial

中图分类号:

TQ35

周凌云, 余小红, 耿圆圆, 华燕. 小远志中口山酮类化学成分及生物活性研究[J]. 林产化学与工业, 2017, 37(2): 121-128.

ZHOU Lingyun, YU Xiaohong, GENG Yuanyuan, HUA Yan. Chemical Constituents and Bioactivities of Xanthones from Polygala sibirica L. var. megalopha Fr.[J]. Chemistry and Industry of Forest Products, 2017, 37(2): 121-128.

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