大血藤原花色素抗氧化活性及酶抑制活性研究

doi:10.3969/j.issn.0253-2417.2017.03.010

摘要/Abstract

摘要： 用70%乙醇从大血藤中提取原花色素，并对粗提物进行纯化和分离，采用香草醛-盐酸比色法测定各样品中原花色素含量，并测定各样品抗氧化活性和酶抑制活性。结果表明：大血藤乙醇粗提物（CE）、石油醚萃取物（PE）、乙酸乙酯与水两相不溶物（EIF）、乙酸乙酯萃取物（EF）、正丁醇萃取物（BF）和水相留余物（WR）中含原花色素分别为（36.03±0.40）%、（1.19±0.11）%、（57.99±0.22）%、（32.40±0.07）%、（25.03±0.08）%和（41.32±0.37）%。大血藤乙醇粗提物经葡聚糖凝胶LH-20 纯化后，含原花色素（98.35±0.72）%，约为粗提物的2.73倍；大血藤纯化物（PT）对二苯基苦基肼自由基（DPPH·）的半数抑制质量浓度（IC₅₀）为（70.70±1.38） mg/L，是L-抗坏血酸的1.12倍；对α-葡萄糖苷酶的IC₅₀为（4.37±0.17） mg/L是阿卡波糖的120.71倍。说明大血藤含有丰富的原花色素，大血藤原花色素具有较强的体外抗氧化活性和酶抑制活性。

关键词: 原花色素, DPPH自由基清除活性, 糖苷酶抑制活性, 胆碱酯酶抑制活性

Abstract: Proanthocyanidins were extracted from Sargentodoxa cuneata (Oliv) Rehd. et Wils., and the crude extract was purified and isolated. The content of proanthocyanidins was determined by an improved vanillin-HCl colorimetric method. Meanwhile, the antioxidant activity and enzyme inhibitory activity of each sample were measured. The results showed that the contents of proanthocyanidins in the crude extract(CE), petroleum ether fraction(PE), ethyl acetate insoluble fraction(EIF), ethyl acetate fraction(EF), n-butanol fraction(BF) and water residue(WR) were (36.03±0.40)%, (1.19±0.11)%, (57.99±0.22)%, (32.40±0.07)%, (25.03±0.08)% and (41.32±0.37)%, respectively. Moreover, the proanthocyanidin content of the sample purified by Sephadex LH-20 was (98.35±0.72)%, which was 2.73 times of that of the crude extract. The DPPH free radical scavenging activity of the crude extract(IC₅₀=178.10±2.67 mg/L) was 1.12 times of that of L-ascorbic acid(IC₅₀=78.89±1.67 mg/L); the α-glucosidase inhibitory activity of purified sample was 120.71 times of that of acarbose and the corresponding acetylcholine inhibitory activity was 1.14 times of that of tacrine. It showed that there were abundant proanthocyanidins in S. cuneata and the proanthocyanidins had an obvious antioxidant activity and enzyme inhibitory activity in vitro.

Key words: proanthocyanidins, DPPH free radical scavenging activity, glycosidase inhibitory activity, cholinesterase inhibitory activity

中图分类号:

TQ35

李彦, 李鑫, 刘景玲, 邓凯航, 梁宗锁. 大血藤原花色素抗氧化活性及酶抑制活性研究[J]. 林产化学与工业, 2017, 37(3): 73-81.

LI Yan, LI Xin, LIU Jingling, DENG Kaihang, LIANG Zongsuo. Antioxidant Activity and Enzyme Inhibitory Activity of Proanthocyanidins from Sargentodoxa cuneata(Oliv) Rehd.et Wils.[J]. Chemistry and Industry of Forest Products, 2017, 37(3): 73-81.

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