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林产化学与工业 ›› 2017, Vol. 37 ›› Issue (5): 113-118.doi: 10.3969/j.issn.0253-2417.2017.05.015

• 研究报告 • 上一篇    下一篇

竹叶总黄酮对脂肪酸合酶及人乳腺癌细胞的抑制作用

李夏冰1, 金昭君2, 荀航1, 汤锋1, 马晓丰2, 岳永德1   

  1. 1. 国际竹藤中心 国家林业局竹藤科学与技术重点实验室, 北京 100102;
    2. 中国科学院大学 生命科学学院, 北京 101407
  • 收稿日期:2017-03-06 出版日期:2017-10-25 发布日期:2017-10-30
  • 通讯作者: 岳永德,教授,博士生导师,研究领域为竹藤资源化学利用;E-mail:yueyd@icbr.ac.cn。 E-mail:yueyd@icbr.ac.cn
  • 作者简介:李夏冰(1987-),男,安徽安庆人,博士生,主要从事竹藤化学研究工作
  • 基金资助:
    国际竹藤中心基本科研业务经费(1632015012)

Inhibitory Effects of Bamboo Leaves Flavonoids on Fatty Acid Synthase and Human Breast Cancer Cells

LI Xiabing1, JIN Zhaojun2, XUN Hang1, TANG Feng1, MA Xiaofeng2, YUE Yongde1   

  1. 1. International Centre for Bamboo and Rattan, Key Laboratory of Science and Technology of Bamboo and Rattan, State Forestry Administration, Beijing 100102, China;
    2. College of Life Sciences, Univercity of Chinese Academy of Sciences, Beijing 101407, China
  • Received:2017-03-06 Online:2017-10-25 Published:2017-10-30

摘要: 研究了毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮对脂肪酸合酶(FAS)和人乳腺癌细胞(MDA-MB-231)的体外抑制活性。通过紫外分光光度法测定竹叶总黄酮对FAS的抑制作用,CCK-8法检测MDA-MB-231细胞的体外增殖抑制作用,流式细胞仪检测MDA-MB-231细胞凋亡作用。结果表明:毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮粗提物对FAS抑制活性显著,其IC50依次为:112.43、153.29、161.32和195.09 mg/L;4种竹叶总黄酮粗提物对MDA-MB-231细胞具有显著体外抑制活性,且具有良好的量效关系。竹叶总黄酮的主要活性部位为大孔树脂70%和95%乙醇相;苦竹竹叶95%乙醇相对MDA-MB-231细胞抑制活性最强,当质量浓度100、200、400 mg/L时对MDA-MB-231细胞增殖抑制率分别为25%、44%和70%;凋亡率分别为11.1%、23.1%和38.7%。

关键词: 竹叶总黄酮, 脂肪酸合酶, 人乳腺癌细胞MDA-MB-231, 细胞凋亡

Abstract: The inhibitory effects of bamboo leaves flavonoids (BLF) from the species of Pleioblastus amarus (Keng) keng, Indocalamus latifolius (Keng) McClure, Phyllostachys heterocycla (Carr.) Mitford Pubescens and P. glauca McClure on the fatty acid synthase (FAS) and human breast cancer cell MDA-MB-231 in vitro were studied. The inhibitory effects of bamboo leaves flavonoids on FAS were determined by UV spectrophotometry, and the inhibitory effects of MDA-MB-231 were detected by CCK-8 assay. Flow cytometry was used to detect the apoptosis of MDA-MB-231. The results showed that all four bamboo leaves flavonoids had significant inhibitory effects on FAS and MDA-MB-231 cells. The IC50 values of four flavonoids against FAS were 112.43, 153.29, 161.32 and 195.09 mg/L, respectively. The inhibition effects of flavonoids on MDA-MB-231 followed the dose-effect relationship. Further studies showed that the 70% and 95% ethanol fraction of bamboo leaves flavonoids exhibited the strongest cytotoxicity. The inhibition rates against MDA-MB-231 at the mass concentration of 100, 200, 400 mg/L of 95% ethanol fraction of P. amarus were 25%, 44% and 70%, and the apoptosis rates were 11.1%, 23.1% and 38.7%, respectively.

Key words: bamboo leaves flavonoids, FAS, human cacer cells MDA-MB-231, apoptosis

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