超声波法辅助定向降解阿拉伯半乳聚糖的研究

doi:10.3969/j.issn.0253-2417.2018.04.010

林产化学与工业 ›› 2018, Vol. 38 ›› Issue (4): 61-68.doi: 10.3969/j.issn.0253-2417.2018.04.010

超声波法辅助定向降解阿拉伯半乳聚糖的研究

张海芸^1,2, 贺亮², 李琴², 杨波¹, 杨光¹

1. 上海理工大学医疗器械与食品学院, 上海 200093;
2. 浙江省森林资源生物与化学利用重点实验室;浙江省林业科学研究院森林食品所, 浙江杭州 310023

收稿日期:2018-03-19 出版日期:2018-08-25 发布日期:2018-08-10
通讯作者: 杨光,副教授,硕士生导师,研究方向为蛋白质及果蔬加工;E-mail:luck_yang@126.com。 E-mail:luck_yang@126.com
作者简介:张海芸(1992-),女,山东潍坊人,硕士生,主要从事森林食品深加工研究工作;E-mail:1985982622@qq.com
基金资助:
国家重点研发计划资助项目（2016YFD0600803）

Ultrasonic-assisted Radical Process for Directed Degradation of Arabinogalactan

ZHANG Haiyun^1,2, HE Liang², LI Qin², YANG Bo¹, YANG Guang¹

1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
2. Zhengjiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resources, Zhengjiang Forestry Academy, Hangzhou 310023, China

Received:2018-03-19 Online:2018-08-25 Published:2018-08-10

摘要/Abstract

摘要： 以落叶松（Larix gmelinii（Rupr.） Kuzen）的木屑为原料提取阿拉伯半乳聚糖（AG），并利用超声波辅助AG定向降解，以降解后阿拉伯半乳低聚糖（AG-O）对自由基清除率为检测指标，在单因素试验的基础上，再利用响应面优化确定最佳降解条件。研究结果表明：最佳降解条件为多糖质量浓度10 g/L、反应温度60℃、H₂O₂体积分数6%、超声波作用时间60 min，在此条件下获得的阿拉伯半乳低聚糖对·OH清除率为89.6%，且可使原阿拉伯半乳聚糖的分子质量由52 ku定向降解到25 ku左右，且多糖累积质量分数达到60%；AG-O抗氧化活性较AG明显增强，其IC₅₀值由2.058 g/L降低到0.497 g/L。

关键词: 阿拉伯半乳聚糖, 自由基清除率, 响应面优化

Abstract: Arabinogalactan (AG) was extracted from the sawdust of larch (Larix gmelinii(Rupr.) Kuzen). The arabinogalactan obtained was physically degraded by ultrasound-assisted free radicals. The free radical scavenging ability of decomposed AG was selected as an indicator on the evaluation of the antioxidant activity. On the basis of single-factor test, response surface optimization was used to determine the optimal degradation conditions, and the optimum conditions are found as follows:the dpolysaccharide concentration was 10 g/L; ultrasonic temperature was 60℃; the H₂O₂ volume fraction was 6%;and the ultrasonic time was 60 min. Under this optimum degradation condition, the·OH scavenging rate of arabinogalactan was 89.6%, and Mr of AG was degraded from 52 ku to around 25 ku, and the cumulative weight fraction of this molecular weight reached 60%.Compared with AG, the antioxidant activity of arabinogalactan oligosaccharide(AG-O) obtained in the anti-oxidative activity was significantly enhanced, and its IC₅₀ value decreased from 2.058 g/L to 0.497g/L.

Key words: arabinogalactan, free radical scavenging, response surface optimizatio

中图分类号:

TQ35

张海芸, 贺亮, 李琴, 杨波, 杨光. 超声波法辅助定向降解阿拉伯半乳聚糖的研究[J]. 林产化学与工业, 2018, 38(4): 61-68.

ZHANG Haiyun, HE Liang, LI Qin, YANG Bo, YANG Guang. Ultrasonic-assisted Radical Process for Directed Degradation of Arabinogalactan[J]. Chemistry and Industry of Forest Products, 2018, 38(4): 61-68.

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超声波法辅助定向降解阿拉伯半乳聚糖的研究

Ultrasonic-assisted Radical Process for Directed Degradation of Arabinogalactan

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